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Detached leaf in vitro model for masked mycotoxin biosynthesis and subsequent analysis of unknown conjugates

Marthe De Boevre (UGent) , Adriaan Vanheule (UGent) , Kris Audenaert (UGent) , Boris Bekaert (UGent) , José Diana Di Mavungu (UGent) , Stefaan Werbrouck (UGent) , Geert Haesaert (UGent) and Sarah De Saeger (UGent)
(2014) WORLD MYCOTOXIN JOURNAL. 7(3). p.305-312
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Abstract
The manuscript details the development of an in vitro model plant system using detached leaves because there is a need for biosynthetic methods for the production and isolation of masked mycotoxins. This detached leaf in vitro model was firstly applied to deoxynivalenol with satisfying results. The biosynthesis of deoxynivalenol-3-glucoside was confirmed using its respective commercially available reference standard. Secondly, the detached leaf in vitro model was applied to T-2 toxin. Mono- and tri-glucoside derivatives of T-2 toxin and HT-2 toxin, T-2-(3)-glucoside, T-2-(3)-triglucoside and HT-2-(3)-glucoside were identified and characterised using Orbitrap high-resolution mass spectrometry. This is the first report on a triglucoside of T-2 toxin. The discovery of new masked forms implies the importance of the development of analytical methods for their detection, the constitution of toxicity studies, and proving the relevance of their presence in the food and feed chain.
Keywords
Orbitrap, masked mycotoxins, untargeted analysis, high resolution mass spectrometry, detached leaf, TANDEM MASS-SPECTROMETRY, NATURALLY CONTAMINATED WHEAT, FUSARIUM HEAD BLIGHT, UDP-GLUCOSYLTRANSFERASE, ARABIDOPSIS-THALIANA, LC-MS/MS, HT-2 TOXINS, T-2 TOXIN, DEOXYNIVALENOL, RESISTANCE

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Citation

Please use this url to cite or link to this publication:

Chicago
De Boevre, Marthe, Adriaan Vanheule, Kris Audenaert, Boris Bekaert, José Diana Di Mavungu, Stefaan Werbrouck, Geert Haesaert, and Sarah De Saeger. 2014. “Detached Leaf in Vitro Model for Masked Mycotoxin Biosynthesis and Subsequent Analysis of Unknown Conjugates.” World Mycotoxin Journal 7 (3): 305–312.
APA
De Boevre, M., Vanheule, A., Audenaert, K., Bekaert, B., Diana Di Mavungu, J., Werbrouck, S., Haesaert, G., et al. (2014). Detached leaf in vitro model for masked mycotoxin biosynthesis and subsequent analysis of unknown conjugates. WORLD MYCOTOXIN JOURNAL, 7(3), 305–312.
Vancouver
1.
De Boevre M, Vanheule A, Audenaert K, Bekaert B, Diana Di Mavungu J, Werbrouck S, et al. Detached leaf in vitro model for masked mycotoxin biosynthesis and subsequent analysis of unknown conjugates. WORLD MYCOTOXIN JOURNAL. 2014;7(3):305–12.
MLA
De Boevre, Marthe, Adriaan Vanheule, Kris Audenaert, et al. “Detached Leaf in Vitro Model for Masked Mycotoxin Biosynthesis and Subsequent Analysis of Unknown Conjugates.” WORLD MYCOTOXIN JOURNAL 7.3 (2014): 305–312. Print.
@article{5766877,
  abstract     = {The manuscript details the development of an in vitro model plant system using detached leaves because there is a need for biosynthetic methods for the production and isolation of masked mycotoxins. This detached leaf in vitro model was firstly applied to deoxynivalenol with satisfying results. The biosynthesis of deoxynivalenol-3-glucoside was confirmed using its respective commercially available reference standard. Secondly, the detached leaf in vitro model was applied to T-2 toxin. Mono- and tri-glucoside derivatives of T-2 toxin and HT-2 toxin, T-2-(3)-glucoside, T-2-(3)-triglucoside and HT-2-(3)-glucoside were identified and characterised using Orbitrap high-resolution mass spectrometry. This is the first report on a triglucoside of T-2 toxin. The discovery of new masked forms implies the importance of the development of analytical methods for their detection, the constitution of toxicity studies, and proving the relevance of their presence in the food and feed chain.},
  author       = {De Boevre, Marthe and Vanheule, Adriaan and Audenaert, Kris and Bekaert, Boris and Diana Di Mavungu, Jos{\'e} and Werbrouck, Stefaan and Haesaert, Geert and De Saeger, Sarah},
  issn         = {1875-0710},
  journal      = {WORLD MYCOTOXIN JOURNAL},
  keyword      = {Orbitrap,masked mycotoxins,untargeted analysis,high resolution mass spectrometry,detached leaf,TANDEM MASS-SPECTROMETRY,NATURALLY CONTAMINATED WHEAT,FUSARIUM HEAD BLIGHT,UDP-GLUCOSYLTRANSFERASE,ARABIDOPSIS-THALIANA,LC-MS/MS,HT-2 TOXINS,T-2 TOXIN,DEOXYNIVALENOL,RESISTANCE},
  language     = {eng},
  number       = {3},
  pages        = {305--312},
  title        = {Detached leaf in vitro model for masked mycotoxin biosynthesis and subsequent analysis of unknown conjugates},
  url          = {http://dx.doi.org/10.3920/WMJ2014.1717},
  volume       = {7},
  year         = {2014},
}

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