Multiplex lateral flow immunoassay for mycotoxin determination
- Author
- Suquan Song, Na Liu, Zhiyong Zhao, Emmanuel Njumbe Ediage (UGent) , Songling Wu, Changpo Sun, Sarah De Saeger (UGent) and Aibo Wu
- Organization
- Abstract
- A new lateral flow immunoassay (LFA) is proposed for qualitative and/or semiquantitative determination of aflatoxin B1 (AFB1), zearalenone (ZEA), deoxynivalenol (DON), and their analogues (AFs, ZEAs, DONs) in cereal samples. Each of the mycotoxin specific antibody was class specific and there was no cross reactivity to other groups of compounds. The visual limits of detection (vLOD) of the strip were 0.03, 1.6, and 10 mu g/kg for AFB1, ZEA and DON, respectively. The calculated limits of detection (cLOD) were 0.05, 1, and 3 mu g/kg respectively. Meanwhile the cutoff values were achieved at 1, 50, and 60 mu g/kg for AFB1, ZEA and DON, respectively. Recoveries ranged from 80% to 122% and RSD from 5% to 20%. Both the vLOD and CLOD for the three mycotoxins were lower than the EU maximum levels. Analysis of naturally contaminated maize samples resulted in a good agreement between the multiplex LFA and LC-MS/MS (100% for DONs and AFs, and 81% for ZEAs). Careful analysis of the results further explained the general overestimation of LFA compared to chromatographic methods for quantification of mycotoxins.
- Keywords
- ZEAs, cereal samples, DONs, aflatoxin B1 (AFB1), lateral flow immunoassay (LFA), zearalenone (ZEA), deoxynivalenol (DON), IMMUNOCHROMATOGRAPHIC ASSAY, SIMULTANEOUS QUANTIFICATION, MASKED MYCOTOXINS, AFLATOXIN B-1, FUMONISIN B1, ZEARALENONE, DEOXYNIVALENOL, OCHRATOXIN, WHEAT, CORN
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-5766831
- MLA
- Song, Suquan, et al. “Multiplex Lateral Flow Immunoassay for Mycotoxin Determination.” ANALYTICAL CHEMISTRY, vol. 86, no. 10, 2014, pp. 4995–5001, doi:10.1021/ac500540z.
- APA
- Song, S., Liu, N., Zhao, Z., Njumbe Ediage, E., Wu, S., Sun, C., … Wu, A. (2014). Multiplex lateral flow immunoassay for mycotoxin determination. ANALYTICAL CHEMISTRY, 86(10), 4995–5001. https://doi.org/10.1021/ac500540z
- Chicago author-date
- Song, Suquan, Na Liu, Zhiyong Zhao, Emmanuel Njumbe Ediage, Songling Wu, Changpo Sun, Sarah De Saeger, and Aibo Wu. 2014. “Multiplex Lateral Flow Immunoassay for Mycotoxin Determination.” ANALYTICAL CHEMISTRY 86 (10): 4995–5001. https://doi.org/10.1021/ac500540z.
- Chicago author-date (all authors)
- Song, Suquan, Na Liu, Zhiyong Zhao, Emmanuel Njumbe Ediage, Songling Wu, Changpo Sun, Sarah De Saeger, and Aibo Wu. 2014. “Multiplex Lateral Flow Immunoassay for Mycotoxin Determination.” ANALYTICAL CHEMISTRY 86 (10): 4995–5001. doi:10.1021/ac500540z.
- Vancouver
- 1.Song S, Liu N, Zhao Z, Njumbe Ediage E, Wu S, Sun C, et al. Multiplex lateral flow immunoassay for mycotoxin determination. ANALYTICAL CHEMISTRY. 2014;86(10):4995–5001.
- IEEE
- [1]S. Song et al., “Multiplex lateral flow immunoassay for mycotoxin determination,” ANALYTICAL CHEMISTRY, vol. 86, no. 10, pp. 4995–5001, 2014.
@article{5766831, abstract = {{A new lateral flow immunoassay (LFA) is proposed for qualitative and/or semiquantitative determination of aflatoxin B1 (AFB1), zearalenone (ZEA), deoxynivalenol (DON), and their analogues (AFs, ZEAs, DONs) in cereal samples. Each of the mycotoxin specific antibody was class specific and there was no cross reactivity to other groups of compounds. The visual limits of detection (vLOD) of the strip were 0.03, 1.6, and 10 mu g/kg for AFB1, ZEA and DON, respectively. The calculated limits of detection (cLOD) were 0.05, 1, and 3 mu g/kg respectively. Meanwhile the cutoff values were achieved at 1, 50, and 60 mu g/kg for AFB1, ZEA and DON, respectively. Recoveries ranged from 80% to 122% and RSD from 5% to 20%. Both the vLOD and CLOD for the three mycotoxins were lower than the EU maximum levels. Analysis of naturally contaminated maize samples resulted in a good agreement between the multiplex LFA and LC-MS/MS (100% for DONs and AFs, and 81% for ZEAs). Careful analysis of the results further explained the general overestimation of LFA compared to chromatographic methods for quantification of mycotoxins.}}, author = {{Song, Suquan and Liu, Na and Zhao, Zhiyong and Njumbe Ediage, Emmanuel and Wu, Songling and Sun, Changpo and De Saeger, Sarah and Wu, Aibo}}, issn = {{0003-2700}}, journal = {{ANALYTICAL CHEMISTRY}}, keywords = {{ZEAs,cereal samples,DONs,aflatoxin B1 (AFB1),lateral flow immunoassay (LFA),zearalenone (ZEA),deoxynivalenol (DON),IMMUNOCHROMATOGRAPHIC ASSAY,SIMULTANEOUS QUANTIFICATION,MASKED MYCOTOXINS,AFLATOXIN B-1,FUMONISIN B1,ZEARALENONE,DEOXYNIVALENOL,OCHRATOXIN,WHEAT,CORN}}, language = {{eng}}, number = {{10}}, pages = {{4995--5001}}, title = {{Multiplex lateral flow immunoassay for mycotoxin determination}}, url = {{http://doi.org/10.1021/ac500540z}}, volume = {{86}}, year = {{2014}}, }
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