Advanced search
1 file | 474.47 KB Add to list

Simultaneous determination of several mycotoxins by rapid immunofiltration assay

Author
Organization
Abstract
A method is developed for the simultaneous rapid determination of three mycotoxins, zearalenone (ZEN), ochratoxin A (OTA), and fumonisin B1 (Fum) by membrane immunofiltration analysis using a marker enzyme of alkaline phosphatase (AP) and two mycotoxins, deoxynivalenol (DON) and total T-2 toxin (T2) and HT-2 toxin (HT2) with horseradish peroxidase (HRP). The analysis is based on a competitive interaction between the antigene, free and bound to the enzyme, and antibodies immobilized on a membrane. The procedures of membrane fabrication and the conditions of mycotoxin to determination in model mixtures and extracts from wheat, corn, and silage are optimized. The influence of sample preparation on the results of analysis is studied. It is shown that the additives of polymers favor the reduction of the matrix effect in the analysis of complex matrixes using conjugated HRP. The methods developed allow the determination of mycotoxins at a level of the maximal permissible concentrations legislated by EU directives. The corresponding values (mu g/kg) are 50, 2.5, and 500 in wheat; 100, 2.5, and 500 in corn; and 125, 25, and 1250 in silage for the simultaneous quantification of ZEN, OTA, and Fum (AP marker). For the determination of DON and total T2/HT2 with HRP, 1250 (1000) and 100 (500) in wheat and corn(silage). The procedures were validated by the analysis of spiked and naturally contaminated samples. The analysis of 10 samples takes 25 min.
Keywords
alkaline phosphatase, horseradish peroxidase, membrane immunofiltration analysis, rapid assays, mycotoxins, THROUGH ENZYME-IMMUNOASSAY, FLOW-THROUGH, AFLATOXIN B-1, FOOD-PRODUCTS, OCHRATOXIN-A, T-2 TOXIN, SAMPLES, MAIZE, WHEAT, FUMONISINS

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 474.47 KB

Citation

Please use this url to cite or link to this publication:

MLA
Burmistrova, NA, Tyu Rusanova, NA Yurasov, et al. “Simultaneous Determination of Several Mycotoxins by Rapid Immunofiltration Assay.” JOURNAL OF ANALYTICAL CHEMISTRY 69.6 (2014): 525–534. Print.
APA
Burmistrova, NA, Rusanova, Ty., Yurasov, N., De Saeger, S., & Goryacheva, Iy. (2014). Simultaneous determination of several mycotoxins by rapid immunofiltration assay. JOURNAL OF ANALYTICAL CHEMISTRY, 69(6), 525–534.
Chicago author-date
Burmistrova, NA, Tyu Rusanova, NA Yurasov, Sarah De Saeger, and Iyu Goryacheva. 2014. “Simultaneous Determination of Several Mycotoxins by Rapid Immunofiltration Assay.” Journal of Analytical Chemistry 69 (6): 525–534.
Chicago author-date (all authors)
Burmistrova, NA, Tyu Rusanova, NA Yurasov, Sarah De Saeger, and Iyu Goryacheva. 2014. “Simultaneous Determination of Several Mycotoxins by Rapid Immunofiltration Assay.” Journal of Analytical Chemistry 69 (6): 525–534.
Vancouver
1.
Burmistrova N, Rusanova Ty, Yurasov N, De Saeger S, Goryacheva Iy. Simultaneous determination of several mycotoxins by rapid immunofiltration assay. JOURNAL OF ANALYTICAL CHEMISTRY. 2014;69(6):525–34.
IEEE
[1]
N. Burmistrova, Ty. Rusanova, N. Yurasov, S. De Saeger, and Iy. Goryacheva, “Simultaneous determination of several mycotoxins by rapid immunofiltration assay,” JOURNAL OF ANALYTICAL CHEMISTRY, vol. 69, no. 6, pp. 525–534, 2014.
@article{5766819,
  abstract     = {A method is developed for the simultaneous rapid determination of three mycotoxins, zearalenone (ZEN), ochratoxin A (OTA), and fumonisin B1 (Fum) by membrane immunofiltration analysis using a marker enzyme of alkaline phosphatase (AP) and two mycotoxins, deoxynivalenol (DON) and total T-2 toxin (T2) and HT-2 toxin (HT2) with horseradish peroxidase (HRP). The analysis is based on a competitive interaction between the antigene, free and bound to the enzyme, and antibodies immobilized on a membrane. The procedures of membrane fabrication and the conditions of mycotoxin to determination in model mixtures and extracts from wheat, corn, and silage are optimized. The influence of sample preparation on the results of analysis is studied. It is shown that the additives of polymers favor the reduction of the matrix effect in the analysis of complex matrixes using conjugated HRP. The methods developed allow the determination of mycotoxins at a level of the maximal permissible concentrations legislated by EU directives. The corresponding values (mu g/kg) are 50, 2.5, and 500 in wheat; 100, 2.5, and 500 in corn; and 125, 25, and 1250 in silage for the simultaneous quantification of ZEN, OTA, and Fum (AP marker). For the determination of DON and total T2/HT2 with HRP, 1250 (1000) and 100 (500) in wheat and corn(silage). The procedures were validated by the analysis of spiked and naturally contaminated samples. The analysis of 10 samples takes 25 min.},
  author       = {Burmistrova, NA and Rusanova, TYu and Yurasov, NA and De Saeger, Sarah and Goryacheva, IYu},
  issn         = {1061-9348},
  journal      = {JOURNAL OF ANALYTICAL CHEMISTRY},
  keywords     = {alkaline phosphatase,horseradish peroxidase,membrane immunofiltration analysis,rapid assays,mycotoxins,THROUGH ENZYME-IMMUNOASSAY,FLOW-THROUGH,AFLATOXIN B-1,FOOD-PRODUCTS,OCHRATOXIN-A,T-2 TOXIN,SAMPLES,MAIZE,WHEAT,FUMONISINS},
  language     = {eng},
  number       = {6},
  pages        = {525--534},
  title        = {Simultaneous determination of several mycotoxins by rapid immunofiltration assay},
  url          = {http://dx.doi.org/10.1134/S1061934814060045},
  volume       = {69},
  year         = {2014},
}

Altmetric
View in Altmetric
Web of Science
Times cited: