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Hydrophilic, bright CuInS2 quantum dots as Cd-free Fluorescent Labels in Quantitative Immunoassay

Elena Speranskaya (UGent) , Natalia Beloglazova (UGent) , Sofie Abé (UGent) , Tangi Aubert (UGent) , Philippe Smet (UGent) , Dirk Poelman (UGent) , Irina Y Goryacheva, Sarah De Saeger (UGent) and Zeger Hens (UGent)
(2014) LANGMUIR. 30(25). p.7567-7575
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Center for nano- and biophotonics (NB-Photonics)
Abstract
We report on the synthesis of core-shell CuInS2/ZnS quantum dots (QDs) in organic solution, their encapsulation with a PEG-containing amphiphilic polymer, and the application of the resulting water-soluble QDs as fluorescent label in quantitative immunoassay. By optimizing the methods for core synthesis and shell growth, CuInS2/ZnS QDs were obtained with a quantum yield of 50% on average after hydrophilization. After conjugation with an aflatoxin B1-protein derivative, the obtained QDs were used as fluorescent labels in microplate immunoassay for the quantitative determination of the mycotoxin aflatoxin B1. QDs-based immunoassay showed higher sensitivity compared to enzyme-based immunoassay.
Keywords
SEMICONDUCTOR NANOCRYSTALS, BOVINE SERUM-ALBUMIN, CORE/SHELL NANOCRYSTALS, SURFACE MODIFICATION, REACTIVE PROTEIN, IN-VITRO, PHOTOLUMINESCENCE, FLUOROIMMUNOASSAY, NONINJECTION, MONODISPERSE

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Citation

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MLA
Speranskaya, Elena, Natalia Beloglazova, Sofie Abé, et al. “Hydrophilic, Bright CuInS2 Quantum Dots as Cd-free Fluorescent Labels in Quantitative Immunoassay.” LANGMUIR 30.25 (2014): 7567–7575. Print.
APA
Speranskaya, E., Beloglazova, N., Abé, S., Aubert, T., Smet, P., Poelman, D., Goryacheva, I. Y., et al. (2014). Hydrophilic, bright CuInS2 quantum dots as Cd-free Fluorescent Labels in Quantitative Immunoassay. LANGMUIR, 30(25), 7567–7575.
Chicago author-date
Speranskaya, Elena, Natalia Beloglazova, Sofie Abé, Tangi Aubert, Philippe Smet, Dirk Poelman, Irina Y Goryacheva, Sarah De Saeger, and Zeger Hens. 2014. “Hydrophilic, Bright CuInS2 Quantum Dots as Cd-free Fluorescent Labels in Quantitative Immunoassay.” Langmuir 30 (25): 7567–7575.
Chicago author-date (all authors)
Speranskaya, Elena, Natalia Beloglazova, Sofie Abé, Tangi Aubert, Philippe Smet, Dirk Poelman, Irina Y Goryacheva, Sarah De Saeger, and Zeger Hens. 2014. “Hydrophilic, Bright CuInS2 Quantum Dots as Cd-free Fluorescent Labels in Quantitative Immunoassay.” Langmuir 30 (25): 7567–7575.
Vancouver
1.
Speranskaya E, Beloglazova N, Abé S, Aubert T, Smet P, Poelman D, et al. Hydrophilic, bright CuInS2 quantum dots as Cd-free Fluorescent Labels in Quantitative Immunoassay. LANGMUIR. 2014;30(25):7567–75.
IEEE
[1]
E. Speranskaya et al., “Hydrophilic, bright CuInS2 quantum dots as Cd-free Fluorescent Labels in Quantitative Immunoassay,” LANGMUIR, vol. 30, no. 25, pp. 7567–7575, 2014.
@article{5766798,
  abstract     = {We report on the synthesis of core-shell CuInS2/ZnS quantum dots (QDs) in organic solution, their encapsulation with a PEG-containing amphiphilic polymer, and the application of the resulting water-soluble QDs as fluorescent label in quantitative immunoassay. By optimizing the methods for core synthesis and shell growth, CuInS2/ZnS QDs were obtained with a quantum yield of 50% on average after hydrophilization. After conjugation with an aflatoxin B1-protein derivative, the obtained QDs were used as fluorescent labels in microplate immunoassay for the quantitative determination of the mycotoxin aflatoxin B1. QDs-based immunoassay showed higher sensitivity compared to enzyme-based immunoassay.},
  author       = {Speranskaya, Elena and Beloglazova, Natalia and Abé, Sofie and Aubert, Tangi and Smet, Philippe and Poelman, Dirk and Goryacheva, Irina Y and De Saeger, Sarah and Hens, Zeger},
  issn         = {0743-7463},
  journal      = {LANGMUIR},
  keywords     = {SEMICONDUCTOR NANOCRYSTALS,BOVINE SERUM-ALBUMIN,CORE/SHELL NANOCRYSTALS,SURFACE MODIFICATION,REACTIVE PROTEIN,IN-VITRO,PHOTOLUMINESCENCE,FLUOROIMMUNOASSAY,NONINJECTION,MONODISPERSE},
  language     = {eng},
  number       = {25},
  pages        = {7567--7575},
  title        = {Hydrophilic, bright CuInS2 quantum dots as Cd-free Fluorescent Labels in Quantitative Immunoassay},
  url          = {http://dx.doi.org/10.1021/la501268b},
  volume       = {30},
  year         = {2014},
}

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