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Bradyrhizobium manausense sp nov., isolated from effective nodules of Vigna unguiculata grown in Brazilian Amazonian rainforest soils

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Abstract
Root nodule bacteria were trapped within cowpea (Vigna unguiculata) in soils with different cultivation histories collected from the Amazonian rainforest in northern Brazil. Analysis of the 16S rRNA gene sequences of six strains (BR 3351(T), BR 3307, BR 3310, BR 3315, BR 3323 BR and BR 3361) isolated from cowpea nodules showed that they formed a distinct group within the genus Bradyrhizobium, which was separate from previously identified type strains. Phylogenetic analyses of three housekeeping genes (glnII, recA and rpoB) revealed that Bradyrhizobium huanghuaihaiense CCBAU 23303(T) was the most closely related type strain (96 % sequence similarity or lower). Chemotaxonomic data, including fatty acid profiles (predominant fatty acids being C-16:0 and summed feature 8), the slow growth rate and carbon compound utilization patterns supported the assignment of the strains to the genus Bradyrhizobium. The results of DNA-DNA hybridizations, antibiotic resistance and physiological tests differentiated these novel strains from the most closely related species of the genus Bradyrhizobium with validly published names. Symbiosis-related genes for nodulation (nodC) and nitrogen fixation (nifH) grouped the novel strains of the genus Bradyrhizobium together with Bradyrhizobium iriomotense strain EK05(T), with 94 % and 96 % sequence similarity, respectively. Based on these data, these six strains represent a novel species for which the name Bradyrhizobium manausense sp. nov. (BR 3351(T)=HAMBI 3596(T)), is proposed.
Keywords
NITROGEN, FIXATION, LEGUME, SEQUENCES, DNA, DIVERSITY, TRAP PLANT, WESTERN AMAZON, MAXIMUM-LIKELIHOOD, DEOXYRIBONUCLEIC-ACID

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MLA
Silva, Flavia V, Sofie De Meyer, Jean L Simões-Araújo, et al. “Bradyrhizobium Manausense Sp Nov., Isolated from Effective Nodules of Vigna Unguiculata Grown in Brazilian Amazonian Rainforest Soils.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64.7 (2014): 2358–2363. Print.
APA
Silva, F. V., De Meyer, S., Simões-Araújo, J. L., Barbé, T. da C., Xavier, G. R., O’Hara, G., Ardley, J. K., et al. (2014). Bradyrhizobium manausense sp nov., isolated from effective nodules of Vigna unguiculata grown in Brazilian Amazonian rainforest soils. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 64(7), 2358–2363.
Chicago author-date
Silva, Flavia V, Sofie De Meyer, Jean L Simões-Araújo, Tatiane da Costa Barbé, Gustavo R Xavier, Graham O’Hara, Julie K Ardley, Norma G Rumjanek, Anne Willems, and Jerri E Zilli. 2014. “Bradyrhizobium Manausense Sp Nov., Isolated from Effective Nodules of Vigna Unguiculata Grown in Brazilian Amazonian Rainforest Soils.” International Journal of Systematic and Evolutionary Microbiology 64 (7): 2358–2363.
Chicago author-date (all authors)
Silva, Flavia V, Sofie De Meyer, Jean L Simões-Araújo, Tatiane da Costa Barbé, Gustavo R Xavier, Graham O’Hara, Julie K Ardley, Norma G Rumjanek, Anne Willems, and Jerri E Zilli. 2014. “Bradyrhizobium Manausense Sp Nov., Isolated from Effective Nodules of Vigna Unguiculata Grown in Brazilian Amazonian Rainforest Soils.” International Journal of Systematic and Evolutionary Microbiology 64 (7): 2358–2363.
Vancouver
1.
Silva FV, De Meyer S, Simões-Araújo JL, Barbé T da C, Xavier GR, O’Hara G, et al. Bradyrhizobium manausense sp nov., isolated from effective nodules of Vigna unguiculata grown in Brazilian Amazonian rainforest soils. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2014;64(7):2358–63.
IEEE
[1]
F. V. Silva et al., “Bradyrhizobium manausense sp nov., isolated from effective nodules of Vigna unguiculata grown in Brazilian Amazonian rainforest soils,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 64, no. 7, pp. 2358–2363, 2014.
@article{5761759,
  abstract     = {Root nodule bacteria were trapped within cowpea (Vigna unguiculata) in soils with different cultivation histories collected from the Amazonian rainforest in northern Brazil. Analysis of the 16S rRNA gene sequences of six strains (BR 3351(T), BR 3307, BR 3310, BR 3315, BR 3323 BR and BR 3361) isolated from cowpea nodules showed that they formed a distinct group within the genus Bradyrhizobium, which was separate from previously identified type strains. Phylogenetic analyses of three housekeeping genes (glnII, recA and rpoB) revealed that Bradyrhizobium huanghuaihaiense CCBAU 23303(T) was the most closely related type strain (96 % sequence similarity or lower). Chemotaxonomic data, including fatty acid profiles (predominant fatty acids being C-16:0 and summed feature 8), the slow growth rate and carbon compound utilization patterns supported the assignment of the strains to the genus Bradyrhizobium. The results of DNA-DNA hybridizations, antibiotic resistance and physiological tests differentiated these novel strains from the most closely related species of the genus Bradyrhizobium with validly published names. Symbiosis-related genes for nodulation (nodC) and nitrogen fixation (nifH) grouped the novel strains of the genus Bradyrhizobium together with Bradyrhizobium iriomotense strain EK05(T), with 94 % and 96 % sequence similarity, respectively. Based on these data, these six strains represent a novel species for which the name Bradyrhizobium manausense sp. nov. (BR 3351(T)=HAMBI 3596(T)), is proposed.},
  author       = {Silva, Flavia V and De Meyer, Sofie and Simões-Araújo, Jean L and Barbé, Tatiane da Costa and Xavier, Gustavo R and O'Hara, Graham and Ardley, Julie K and Rumjanek, Norma G and Willems, Anne and Zilli, Jerri E},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keywords     = {NITROGEN,FIXATION,LEGUME,SEQUENCES,DNA,DIVERSITY,TRAP PLANT,WESTERN AMAZON,MAXIMUM-LIKELIHOOD,DEOXYRIBONUCLEIC-ACID},
  language     = {eng},
  number       = {7},
  pages        = {2358--2363},
  title        = {Bradyrhizobium manausense sp nov., isolated from effective nodules of Vigna unguiculata grown in Brazilian Amazonian rainforest soils},
  url          = {http://dx.doi.org/10.1099/ijs.0.061259-0},
  volume       = {64},
  year         = {2014},
}

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