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High-resolution time-resolved imaging of in vitro Arabidopsis rosette growth

Stijn Dhondt UGent, Nathalie Gonzalez Sanchez, Jonas Blomme UGent, Liesbeth De Milde UGent, Twiggy Van Daele UGent, Dirk Van Akoleyen, Veronique Storme UGent, Frederik Coppens UGent, Gerrit Beemster UGent and Dirk Inzé UGent (2014) PLANT JOURNAL. 80(1). p.172-184
abstract
Although quantitative characterization of growth phenotypes is of key importance for the understanding of essential networks driving plant growth, the majority of growth-related genes are still being identified based on qualitative visual observations and/or single-endpoint quantitative measurements. We developed an in vitro growth imaging system (IGIS) to perform time-resolved analysis of rosette growth. In this system, Arabidopsis plants are grown in Petri dishes mounted on a rotating disk, and images of each plate are taken on an hourly basis. Automated image analysis was developed in order to obtain several growth-related parameters, such as projected rosette area, rosette relative growth rate, compactness and stockiness, over time. To illustrate the use of the platform and the resulting data, we present the results for the growth response of Col-0 plants subjected to three mild stress conditions. Although the reduction in rosette area was relatively similar at 19days after stratification, the time-lapse analysis demonstrated that plants react differently to salt, osmotic and oxidative stress. The rosette area was altered at various time points during development, and leaf movement and shape parameters were also affected differently. We also used the IGIS to analyze in detail the growth behavior of mutants with enhanced leaf size. Analysis of several growth-related parameters over time in these mutants revealed several specificities in growth behavior, underlining the high complexity of leaf growth coordination. These results demonstrate that time-resolved imaging of in vitro rosette growth generates a better understanding of growth phenotypes than endpoint measurements.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
THALIANA, ANALYSIS TOOL, SIZE, EXPANSION, technical advance, leaf movement, imaging, growth dynamics, phenotyping platform, rosette, PLANT, Arabidopsis thaliana, HYPOCOTYL GROWTH, CELL-PROLIFERATION, LEAF GROWTH, ROOT, SHADE AVOIDANCE
journal title
PLANT JOURNAL
Plant J.
volume
80
issue
1
pages
172 - 184
Web of Science type
Article
Web of Science id
000342721900014
JCR category
PLANT SCIENCES
JCR impact factor
5.972 (2014)
JCR rank
10/204 (2014)
JCR quartile
1 (2014)
ISSN
0960-7412
DOI
10.1111/tpj.12610
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5756270
handle
http://hdl.handle.net/1854/LU-5756270
date created
2014-11-17 15:57:56
date last changed
2016-12-19 15:39:18
@article{5756270,
  abstract     = {Although quantitative characterization of growth phenotypes is of key importance for the understanding of essential networks driving plant growth, the majority of growth-related genes are still being identified based on qualitative visual observations and/or single-endpoint quantitative measurements. We developed an in vitro growth imaging system (IGIS) to perform time-resolved analysis of rosette growth. In this system, Arabidopsis plants are grown in Petri dishes mounted on a rotating disk, and images of each plate are taken on an hourly basis. Automated image analysis was developed in order to obtain several growth-related parameters, such as projected rosette area, rosette relative growth rate, compactness and stockiness, over time. To illustrate the use of the platform and the resulting data, we present the results for the growth response of Col-0 plants subjected to three mild stress conditions. Although the reduction in rosette area was relatively similar at 19days after stratification, the time-lapse analysis demonstrated that plants react differently to salt, osmotic and oxidative stress. The rosette area was altered at various time points during development, and leaf movement and shape parameters were also affected differently. We also used the IGIS to analyze in detail the growth behavior of mutants with enhanced leaf size. Analysis of several growth-related parameters over time in these mutants revealed several specificities in growth behavior, underlining the high complexity of leaf growth coordination. These results demonstrate that time-resolved imaging of in vitro rosette growth generates a better understanding of growth phenotypes than endpoint measurements.},
  author       = {Dhondt, Stijn and Gonzalez Sanchez, Nathalie and Blomme, Jonas and De Milde, Liesbeth and Van Daele, Twiggy and Van Akoleyen, Dirk and Storme, Veronique and Coppens, Frederik and Beemster, Gerrit and Inz{\'e}, Dirk},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {THALIANA,ANALYSIS TOOL,SIZE,EXPANSION,technical advance,leaf movement,imaging,growth dynamics,phenotyping platform,rosette,PLANT,Arabidopsis thaliana,HYPOCOTYL GROWTH,CELL-PROLIFERATION,LEAF GROWTH,ROOT,SHADE AVOIDANCE},
  language     = {eng},
  number       = {1},
  pages        = {172--184},
  title        = {High-resolution time-resolved imaging of in vitro Arabidopsis rosette growth},
  url          = {http://dx.doi.org/10.1111/tpj.12610},
  volume       = {80},
  year         = {2014},
}

Chicago
Dhondt, Stijn, Nathalie Gonzalez Sanchez, Jonas Blomme, Liesbeth De Milde, Twiggy Van Daele, Dirk Van Akoleyen, Veronique Storme, Frederik Coppens, Gerrit Beemster, and Dirk Inzé. 2014. “High-resolution Time-resolved Imaging of in Vitro Arabidopsis Rosette Growth.” Plant Journal 80 (1): 172–184.
APA
Dhondt, S., Gonzalez Sanchez, N., Blomme, J., De Milde, L., Van Daele, T., Van Akoleyen, D., Storme, V., et al. (2014). High-resolution time-resolved imaging of in vitro Arabidopsis rosette growth. PLANT JOURNAL, 80(1), 172–184.
Vancouver
1.
Dhondt S, Gonzalez Sanchez N, Blomme J, De Milde L, Van Daele T, Van Akoleyen D, et al. High-resolution time-resolved imaging of in vitro Arabidopsis rosette growth. PLANT JOURNAL. 2014;80(1):172–84.
MLA
Dhondt, Stijn, Nathalie Gonzalez Sanchez, Jonas Blomme, et al. “High-resolution Time-resolved Imaging of in Vitro Arabidopsis Rosette Growth.” PLANT JOURNAL 80.1 (2014): 172–184. Print.