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High-resolution time-resolved imaging of in vitro Arabidopsis rosette growth

Stijn Dhondt (UGent) , Nathalie Gonzalez Sanchez (UGent) , Jonas Blomme (UGent) , Liesbeth De Milde (UGent) , Twiggy Van Daele (UGent) , Dirk Van Akoleyen (UGent) , Veronique Storme (UGent) , Frederik Coppens (UGent) , Gerrit Beemster (UGent) and Dirk Inzé (UGent)
(2014) PLANT JOURNAL. 80(1). p.172-184
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Abstract
Although quantitative characterization of growth phenotypes is of key importance for the understanding of essential networks driving plant growth, the majority of growth-related genes are still being identified based on qualitative visual observations and/or single-endpoint quantitative measurements. We developed an in vitro growth imaging system (IGIS) to perform time-resolved analysis of rosette growth. In this system, Arabidopsis plants are grown in Petri dishes mounted on a rotating disk, and images of each plate are taken on an hourly basis. Automated image analysis was developed in order to obtain several growth-related parameters, such as projected rosette area, rosette relative growth rate, compactness and stockiness, over time. To illustrate the use of the platform and the resulting data, we present the results for the growth response of Col-0 plants subjected to three mild stress conditions. Although the reduction in rosette area was relatively similar at 19days after stratification, the time-lapse analysis demonstrated that plants react differently to salt, osmotic and oxidative stress. The rosette area was altered at various time points during development, and leaf movement and shape parameters were also affected differently. We also used the IGIS to analyze in detail the growth behavior of mutants with enhanced leaf size. Analysis of several growth-related parameters over time in these mutants revealed several specificities in growth behavior, underlining the high complexity of leaf growth coordination. These results demonstrate that time-resolved imaging of in vitro rosette growth generates a better understanding of growth phenotypes than endpoint measurements.
Keywords
THALIANA, ANALYSIS TOOL, SIZE, EXPANSION, technical advance, leaf movement, imaging, growth dynamics, phenotyping platform, rosette, PLANT, Arabidopsis thaliana, HYPOCOTYL GROWTH, CELL-PROLIFERATION, LEAF GROWTH, ROOT, SHADE AVOIDANCE

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MLA
Dhondt, Stijn, et al. “High-Resolution Time-Resolved Imaging of in Vitro Arabidopsis Rosette Growth.” PLANT JOURNAL, vol. 80, no. 1, 2014, pp. 172–84, doi:10.1111/tpj.12610.
APA
Dhondt, S., Gonzalez Sanchez, N., Blomme, J., De Milde, L., Van Daele, T., Van Akoleyen, D., … Inzé, D. (2014). High-resolution time-resolved imaging of in vitro Arabidopsis rosette growth. PLANT JOURNAL, 80(1), 172–184. https://doi.org/10.1111/tpj.12610
Chicago author-date
Dhondt, Stijn, Nathalie Gonzalez Sanchez, Jonas Blomme, Liesbeth De Milde, Twiggy Van Daele, Dirk Van Akoleyen, Veronique Storme, Frederik Coppens, Gerrit Beemster, and Dirk Inzé. 2014. “High-Resolution Time-Resolved Imaging of in Vitro Arabidopsis Rosette Growth.” PLANT JOURNAL 80 (1): 172–84. https://doi.org/10.1111/tpj.12610.
Chicago author-date (all authors)
Dhondt, Stijn, Nathalie Gonzalez Sanchez, Jonas Blomme, Liesbeth De Milde, Twiggy Van Daele, Dirk Van Akoleyen, Veronique Storme, Frederik Coppens, Gerrit Beemster, and Dirk Inzé. 2014. “High-Resolution Time-Resolved Imaging of in Vitro Arabidopsis Rosette Growth.” PLANT JOURNAL 80 (1): 172–184. doi:10.1111/tpj.12610.
Vancouver
1.
Dhondt S, Gonzalez Sanchez N, Blomme J, De Milde L, Van Daele T, Van Akoleyen D, et al. High-resolution time-resolved imaging of in vitro Arabidopsis rosette growth. PLANT JOURNAL. 2014;80(1):172–84.
IEEE
[1]
S. Dhondt et al., “High-resolution time-resolved imaging of in vitro Arabidopsis rosette growth,” PLANT JOURNAL, vol. 80, no. 1, pp. 172–184, 2014.
@article{5756270,
  abstract     = {{Although quantitative characterization of growth phenotypes is of key importance for the understanding of essential networks driving plant growth, the majority of growth-related genes are still being identified based on qualitative visual observations and/or single-endpoint quantitative measurements. We developed an in vitro growth imaging system (IGIS) to perform time-resolved analysis of rosette growth. In this system, Arabidopsis plants are grown in Petri dishes mounted on a rotating disk, and images of each plate are taken on an hourly basis. Automated image analysis was developed in order to obtain several growth-related parameters, such as projected rosette area, rosette relative growth rate, compactness and stockiness, over time. To illustrate the use of the platform and the resulting data, we present the results for the growth response of Col-0 plants subjected to three mild stress conditions. Although the reduction in rosette area was relatively similar at 19days after stratification, the time-lapse analysis demonstrated that plants react differently to salt, osmotic and oxidative stress. The rosette area was altered at various time points during development, and leaf movement and shape parameters were also affected differently. We also used the IGIS to analyze in detail the growth behavior of mutants with enhanced leaf size. Analysis of several growth-related parameters over time in these mutants revealed several specificities in growth behavior, underlining the high complexity of leaf growth coordination. These results demonstrate that time-resolved imaging of in vitro rosette growth generates a better understanding of growth phenotypes than endpoint measurements.}},
  author       = {{Dhondt, Stijn and Gonzalez Sanchez, Nathalie and Blomme, Jonas and De Milde, Liesbeth and Van Daele, Twiggy and Van Akoleyen, Dirk and Storme, Veronique and Coppens, Frederik and Beemster, Gerrit and Inzé, Dirk}},
  issn         = {{0960-7412}},
  journal      = {{PLANT JOURNAL}},
  keywords     = {{THALIANA,ANALYSIS TOOL,SIZE,EXPANSION,technical advance,leaf movement,imaging,growth dynamics,phenotyping platform,rosette,PLANT,Arabidopsis thaliana,HYPOCOTYL GROWTH,CELL-PROLIFERATION,LEAF GROWTH,ROOT,SHADE AVOIDANCE}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{172--184}},
  title        = {{High-resolution time-resolved imaging of in vitro Arabidopsis rosette growth}},
  url          = {{http://dx.doi.org/10.1111/tpj.12610}},
  volume       = {{80}},
  year         = {{2014}},
}
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