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Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis

Satoshi Naramoto UGent, Marisa S Otegui, Natsumaro Kutsuna, Riet De Rycke UGent, Tomoko Dainobu, Michail Karampelias, Masaru Fujimoto, Elena Feraru UGent, Daisuke Miki UGent, Hiroo Fukuda, et al. (2014) PLANT CELL. 26(7). p.3062-3076
abstract
GNOM is one of the most characterized membrane trafficking regulators in plants, with crucial roles in development. GNOM encodes an ARF-guanine nucleotide exchange factor (ARF-GEF) that activates small GTPases of the ARF (ADP ribosylation factor) class to mediate vesicle budding at endomembranes. The crucial role of GNOM in recycling of PIN auxin transporters and other proteins to the plasma membrane was identified in studies using the ARF-GEF inhibitor brefeldin A (BFA). GNOM, the most prominent regulator of recycling in plants, has been proposed to act and localize at so far elusive recycling endosomes. Here, we report the GNOM localization in context of its cellular function in Arabidopsis thaliana. State-of-the-art imaging, pharmacological interference, and ultrastructure analysis show that GNOM predominantly localizes to Golgi apparatus. Super-resolution confocal live imaging microscopy identified GNOM and its closest homolog GNOM-like 1 at distinct subdomains on Golgi cisternae. Short-term BFA treatment stabilizes GNOM at the Golgi apparatus, whereas prolonged exposures results in GNOM translocation to trans-Golgi network (TGN)/early endosomes (EEs). Malformed TGN/EE in gnom mutants suggests a role for GNOM in maintaining TGN/EE function. Our results redefine the subcellular action of GNOM and reevaluate the identity and function of recycling endosomes in plants.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SEC7 DOMAIN, EFFLUX CARRIER, PLASMA-MEMBRANE, PLANT-CELLS, PROTEIN TRAFFICKING, VASCULAR NETWORK, EARLY ENDOSOMES, DEPENDENT AUXIN GRADIENTS, BREFELDIN-A, NUCLEOTIDE-EXCHANGE FACTORS
journal title
PLANT CELL
Plant Cell
volume
26
issue
7
pages
3062 - 3076
Web of Science type
Article
Web of Science id
000342076200027
JCR category
PLANT SCIENCES
JCR impact factor
9.338 (2014)
JCR rank
4/204 (2014)
JCR quartile
1 (2014)
ISSN
1040-4651
DOI
10.1105/tpc.114.125880
language
English
UGent publication?
yes
classification
A1
additional info
The first and the last author contributed equally to this work
copyright statement
I have transferred the copyright for this publication to the publisher
id
5738648
handle
http://hdl.handle.net/1854/LU-5738648
date created
2014-10-27 14:49:36
date last changed
2016-12-19 15:46:57
@article{5738648,
  abstract     = {GNOM is one of the most characterized membrane trafficking regulators in plants, with crucial roles in development. GNOM encodes an ARF-guanine nucleotide exchange factor (ARF-GEF) that activates small GTPases of the ARF (ADP ribosylation factor) class to mediate vesicle budding at endomembranes. The crucial role of GNOM in recycling of PIN auxin transporters and other proteins to the plasma membrane was identified in studies using the ARF-GEF inhibitor brefeldin A (BFA). GNOM, the most prominent regulator of recycling in plants, has been proposed to act and localize at so far elusive recycling endosomes. Here, we report the GNOM localization in context of its cellular function in Arabidopsis thaliana. State-of-the-art imaging, pharmacological interference, and ultrastructure analysis show that GNOM predominantly localizes to Golgi apparatus. Super-resolution confocal live imaging microscopy identified GNOM and its closest homolog GNOM-like 1 at distinct subdomains on Golgi cisternae. Short-term BFA treatment stabilizes GNOM at the Golgi apparatus, whereas prolonged exposures results in GNOM translocation to trans-Golgi network (TGN)/early endosomes (EEs). Malformed TGN/EE in gnom mutants suggests a role for GNOM in maintaining TGN/EE function. Our results redefine the subcellular action of GNOM and reevaluate the identity and function of recycling endosomes in plants.},
  author       = {Naramoto, Satoshi and Otegui, Marisa S and Kutsuna, Natsumaro and De Rycke, Riet and Dainobu, Tomoko and Karampelias, Michail and Fujimoto, Masaru and Feraru, Elena and Miki, Daisuke and Fukuda, Hiroo and Nakano, Akihiko and Friml, Jiri},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {SEC7 DOMAIN,EFFLUX CARRIER,PLASMA-MEMBRANE,PLANT-CELLS,PROTEIN TRAFFICKING,VASCULAR NETWORK,EARLY ENDOSOMES,DEPENDENT AUXIN GRADIENTS,BREFELDIN-A,NUCLEOTIDE-EXCHANGE FACTORS},
  language     = {eng},
  number       = {7},
  pages        = {3062--3076},
  title        = {Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis},
  url          = {http://dx.doi.org/10.1105/tpc.114.125880},
  volume       = {26},
  year         = {2014},
}

Chicago
Naramoto, Satoshi, Marisa S Otegui, Natsumaro Kutsuna, Riet De Rycke, Tomoko Dainobu, Michail Karampelias, Masaru Fujimoto, et al. 2014. “Insights into the Localization and Function of the Membrane Trafficking Regulator GNOM ARF-GEF at the Golgi Apparatus in Arabidopsis.” Plant Cell 26 (7): 3062–3076.
APA
Naramoto, S., Otegui, M. S., Kutsuna, N., De Rycke, R., Dainobu, T., Karampelias, M., Fujimoto, M., et al. (2014). Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis. PLANT CELL, 26(7), 3062–3076.
Vancouver
1.
Naramoto S, Otegui MS, Kutsuna N, De Rycke R, Dainobu T, Karampelias M, et al. Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis. PLANT CELL. 2014;26(7):3062–76.
MLA
Naramoto, Satoshi, Marisa S Otegui, Natsumaro Kutsuna, et al. “Insights into the Localization and Function of the Membrane Trafficking Regulator GNOM ARF-GEF at the Golgi Apparatus in Arabidopsis.” PLANT CELL 26.7 (2014): 3062–3076. Print.