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The cyclin-dependent kinase inhibitor KRP6 induces mitosis and impairs cytokinesis in giant cells induced by plant-parasitic nematodes in Arabidopsis

Paulo Vieira, Annelies De Clercq UGent, Hilde Stals UGent, Jelle Van Leene UGent, Eveline Van De Slijke UGent, Gert Van Isterdael UGent, Dominique Eeckhout UGent, Geert Persiau UGent, Daniël Van Damme UGent, Aurine Verkest, et al. (2014) PLANT CELL. 26(6). p.2633-2647
abstract
In Arabidopsis thaliana, seven cyclin-dependent kinase (CDK) inhibitors have been identified, designated interactors of CDKs or Kip-related proteins (KRPs). Here, the function of KRP6 was investigated during cell cycle progression in roots infected by plant-parasitic root-knot nematodes. Contrary to expectations, analysis of Meloidogyne incognita-induced galls of KRP6-overexpressing lines revealed a role for this particular KRP as an activator of the mitotic cell cycle. In accordance, KRP6-overexpressing suspension cultures displayed accelerated entry into mitosis, but delayed mitotic progression. Likewise, phenotypic analysis of cultured cells and nematode-induced giant cells revealed a failure in mitotic exit, with the appearance of multinucleated cells as a consequence. Strong KRP6 expression upon nematode infection and the phenotypic resemblance between KRP6 overexpression cell cultures and root-knot morphology point toward the involvement of KRP6 in the multinucleate and acytokinetic state of giant cells. Along these lines, the parasite might have evolved to manipulate plant KRP6 transcription to the benefit of gall establishment.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
FEEDING SITES, THALIANA, ICK1, KIP-RELATED PROTEINS, ENDOREDUPLICATION, GENE-EXPRESSION, CDK INHIBITORS, TOBACCO BY-2 CELLS, GENOME-WIDE ANALYSIS, ROOT-KNOT NEMATODES
journal title
PLANT CELL
Plant Cell
volume
26
issue
6
pages
2633 - 2647
Web of Science type
Article
Web of Science id
000341884000023
JCR category
PLANT SCIENCES
JCR impact factor
9.338 (2014)
JCR rank
4/204 (2014)
JCR quartile
1 (2014)
ISSN
1040-4651
DOI
10.1105/tpc.114.126425
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
additional info
The first three authors contributed equally to this work. The last four authors share senior authorship.
copyright statement
I have transferred the copyright for this publication to the publisher
id
5738438
handle
http://hdl.handle.net/1854/LU-5738438
date created
2014-10-27 14:28:16
date last changed
2016-12-19 15:40:58
@article{5738438,
  abstract     = {In Arabidopsis thaliana, seven cyclin-dependent kinase (CDK) inhibitors have been identified, designated interactors of CDKs or Kip-related proteins (KRPs). Here, the function of KRP6 was investigated during cell cycle progression in roots infected by plant-parasitic root-knot nematodes. Contrary to expectations, analysis of Meloidogyne incognita-induced galls of KRP6-overexpressing lines revealed a role for this particular KRP as an activator of the mitotic cell cycle. In accordance, KRP6-overexpressing suspension cultures displayed accelerated entry into mitosis, but delayed mitotic progression. Likewise, phenotypic analysis of cultured cells and nematode-induced giant cells revealed a failure in mitotic exit, with the appearance of multinucleated cells as a consequence. Strong KRP6 expression upon nematode infection and the phenotypic resemblance between KRP6 overexpression cell cultures and root-knot morphology point toward the involvement of KRP6 in the multinucleate and acytokinetic state of giant cells. Along these lines, the parasite might have evolved to manipulate plant KRP6 transcription to the benefit of gall establishment.},
  author       = {Vieira, Paulo and De Clercq, Annelies and Stals, Hilde and Van Leene, Jelle and Van De Slijke, Eveline and Van Isterdael, Gert and Eeckhout, Dominique and Persiau, Geert and Van Damme, Dani{\"e}l and Verkest, Aurine and Antonino de Souza J{\'u}nior, Jos{\'e} Dijair and Glab, Nathalie and Abad, Pierre and Engler, Gilbert and Inz{\'e}, Dirk and De Veylder, Lieven and De Jaeger, Geert and Engler, Janice de Almeida},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {FEEDING SITES,THALIANA,ICK1,KIP-RELATED PROTEINS,ENDOREDUPLICATION,GENE-EXPRESSION,CDK INHIBITORS,TOBACCO BY-2 CELLS,GENOME-WIDE ANALYSIS,ROOT-KNOT NEMATODES},
  language     = {eng},
  number       = {6},
  pages        = {2633--2647},
  title        = {The cyclin-dependent kinase inhibitor KRP6 induces mitosis and impairs cytokinesis in giant cells induced by plant-parasitic nematodes in Arabidopsis},
  url          = {http://dx.doi.org/10.1105/tpc.114.126425},
  volume       = {26},
  year         = {2014},
}

Chicago
Vieira, Paulo, Annelies De Clercq, Hilde Stals, Jelle Van Leene, Eveline Van De Slijke, Gert Van Isterdael, Dominique Eeckhout, et al. 2014. “The Cyclin-dependent Kinase Inhibitor KRP6 Induces Mitosis and Impairs Cytokinesis in Giant Cells Induced by Plant-parasitic Nematodes in Arabidopsis.” Plant Cell 26 (6): 2633–2647.
APA
Vieira, Paulo, De Clercq, A., Stals, H., Van Leene, J., Van De Slijke, E., Van Isterdael, G., Eeckhout, D., et al. (2014). The cyclin-dependent kinase inhibitor KRP6 induces mitosis and impairs cytokinesis in giant cells induced by plant-parasitic nematodes in Arabidopsis. PLANT CELL, 26(6), 2633–2647.
Vancouver
1.
Vieira P, De Clercq A, Stals H, Van Leene J, Van De Slijke E, Van Isterdael G, et al. The cyclin-dependent kinase inhibitor KRP6 induces mitosis and impairs cytokinesis in giant cells induced by plant-parasitic nematodes in Arabidopsis. PLANT CELL. 2014;26(6):2633–47.
MLA
Vieira, Paulo, Annelies De Clercq, Hilde Stals, et al. “The Cyclin-dependent Kinase Inhibitor KRP6 Induces Mitosis and Impairs Cytokinesis in Giant Cells Induced by Plant-parasitic Nematodes in Arabidopsis.” PLANT CELL 26.6 (2014): 2633–2647. Print.