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The cyclin-dependent kinase inhibitor KRP6 induces mitosis and impairs cytokinesis in giant cells induced by plant-parasitic nematodes in Arabidopsis

Paulo Vieira, Annelies De Clercq (UGent) , Hilde Stals (UGent) , Jelle Van Leene (UGent) , Eveline Van De Slijke (UGent) , Gert Van Isterdael (UGent) , Dominique Eeckhout (UGent) , Geert Persiau (UGent) , Daniël Van Damme (UGent) , Aurine Verkest (UGent) , et al.
(2014) PLANT CELL. 26(6). p.2633-2647
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
In Arabidopsis thaliana, seven cyclin-dependent kinase (CDK) inhibitors have been identified, designated interactors of CDKs or Kip-related proteins (KRPs). Here, the function of KRP6 was investigated during cell cycle progression in roots infected by plant-parasitic root-knot nematodes. Contrary to expectations, analysis of Meloidogyne incognita-induced galls of KRP6-overexpressing lines revealed a role for this particular KRP as an activator of the mitotic cell cycle. In accordance, KRP6-overexpressing suspension cultures displayed accelerated entry into mitosis, but delayed mitotic progression. Likewise, phenotypic analysis of cultured cells and nematode-induced giant cells revealed a failure in mitotic exit, with the appearance of multinucleated cells as a consequence. Strong KRP6 expression upon nematode infection and the phenotypic resemblance between KRP6 overexpression cell cultures and root-knot morphology point toward the involvement of KRP6 in the multinucleate and acytokinetic state of giant cells. Along these lines, the parasite might have evolved to manipulate plant KRP6 transcription to the benefit of gall establishment.
Keywords
FEEDING SITES, THALIANA, ICK1, KIP-RELATED PROTEINS, ENDOREDUPLICATION, GENE-EXPRESSION, CDK INHIBITORS, TOBACCO BY-2 CELLS, GENOME-WIDE ANALYSIS, ROOT-KNOT NEMATODES

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Citation

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Chicago
Vieira, Paulo, Annelies De Clercq, Hilde Stals, Jelle Van Leene, Eveline Van De Slijke, Gert Van Isterdael, Dominique Eeckhout, et al. 2014. “The Cyclin-dependent Kinase Inhibitor KRP6 Induces Mitosis and Impairs Cytokinesis in Giant Cells Induced by Plant-parasitic Nematodes in Arabidopsis.” Plant Cell 26 (6): 2633–2647.
APA
Vieira, Paulo, De Clercq, A., Stals, H., Van Leene, J., Van De Slijke, E., Van Isterdael, G., Eeckhout, D., et al. (2014). The cyclin-dependent kinase inhibitor KRP6 induces mitosis and impairs cytokinesis in giant cells induced by plant-parasitic nematodes in Arabidopsis. PLANT CELL, 26(6), 2633–2647.
Vancouver
1.
Vieira P, De Clercq A, Stals H, Van Leene J, Van De Slijke E, Van Isterdael G, et al. The cyclin-dependent kinase inhibitor KRP6 induces mitosis and impairs cytokinesis in giant cells induced by plant-parasitic nematodes in Arabidopsis. PLANT CELL. 2014;26(6):2633–47.
MLA
Vieira, Paulo, Annelies De Clercq, Hilde Stals, et al. “The Cyclin-dependent Kinase Inhibitor KRP6 Induces Mitosis and Impairs Cytokinesis in Giant Cells Induced by Plant-parasitic Nematodes in Arabidopsis.” PLANT CELL 26.6 (2014): 2633–2647. Print.
@article{5738438,
  abstract     = {In Arabidopsis thaliana, seven cyclin-dependent kinase (CDK) inhibitors have been identified, designated interactors of CDKs or Kip-related proteins (KRPs). Here, the function of KRP6 was investigated during cell cycle progression in roots infected by plant-parasitic root-knot nematodes. Contrary to expectations, analysis of Meloidogyne incognita-induced galls of KRP6-overexpressing lines revealed a role for this particular KRP as an activator of the mitotic cell cycle. In accordance, KRP6-overexpressing suspension cultures displayed accelerated entry into mitosis, but delayed mitotic progression. Likewise, phenotypic analysis of cultured cells and nematode-induced giant cells revealed a failure in mitotic exit, with the appearance of multinucleated cells as a consequence. Strong KRP6 expression upon nematode infection and the phenotypic resemblance between KRP6 overexpression cell cultures and root-knot morphology point toward the involvement of KRP6 in the multinucleate and acytokinetic state of giant cells. Along these lines, the parasite might have evolved to manipulate plant KRP6 transcription to the benefit of gall establishment.},
  author       = {Vieira, Paulo and De Clercq, Annelies and Stals, Hilde and Van Leene, Jelle and Van De Slijke, Eveline and Van Isterdael, Gert and Eeckhout, Dominique and Persiau, Geert and Van Damme, Dani{\"e}l and Verkest, Aurine and Antonino de Souza J{\'u}nior, Jos{\'e} Dijair and Glab, Nathalie and Abad, Pierre and Engler, Gilbert and Inz{\'e}, Dirk and De Veylder, Lieven and De Jaeger, Geert and Engler, Janice de Almeida},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {FEEDING SITES,THALIANA,ICK1,KIP-RELATED PROTEINS,ENDOREDUPLICATION,GENE-EXPRESSION,CDK INHIBITORS,TOBACCO BY-2 CELLS,GENOME-WIDE ANALYSIS,ROOT-KNOT NEMATODES},
  language     = {eng},
  number       = {6},
  pages        = {2633--2647},
  title        = {The cyclin-dependent kinase inhibitor KRP6 induces mitosis and impairs cytokinesis in giant cells induced by plant-parasitic nematodes in Arabidopsis},
  url          = {http://dx.doi.org/10.1105/tpc.114.126425},
  volume       = {26},
  year         = {2014},
}

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