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Chloroplast dysfunction causes multiple defects in cell cycle progression in the Arabidopsis crumpled leaf mutant

(2014) PLANT PHYSIOLOGY. 166(1). p.152-167
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Abstract
The majority of research on cell cycle regulation is focused on the nuclear events that govern the replication and segregation of the genome between the two daughter cells. However, eukaryotic cells contain several compartmentalized organelles with specialized functions, and coordination among these organelles is required for proper cell cycle progression, as evidenced by the isolation of several mutants in which both organelle function and overall plant development were affected. To investigate how chloroplast dysfunction affects the cell cycle, we analyzed the crumpled leaf (crl) mutant of Arabidopsis (Arabidopsis thaliana), which is deficient for a chloroplastic protein and displays particularly severe developmental defects. In the crl mutant, we reveal that cell cycle regulation is altered drastically and that meristematic cells prematurely enter differentiation, leading to reduced plant stature and early endoreduplication in the leaves. This response is due to the repression of several key cell cycle regulators as well as constitutive activation of stress-response genes, among them the cell cycle inhibitor SIAMESE-RELATED5. One unique feature of the crl mutant is that it produces aplastidic cells in several organs, including the root tip. By investigating the consequence of the absence of plastids on cell cycle progression, we showed that nuclear DNA replication occurs in aplastidic cells in the root tip, which opens future research prospects regarding the dialogue between plastids and the nucleus during cell cycle regulation in higher plants.
Keywords
PLASTIDS, SIGNALS, PROLIFERATION, NUCLEAR GENE-EXPRESSION, DNA-REPLICATION, SINGLET OXYGEN, PLANT-CELL, STRESS RESPONSES, DIVISION, THALIANA

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MLA
Hudik, Elodie, Yasushi Yoshioka, Séverine Domenichini, et al. “Chloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis Crumpled Leaf Mutant.” PLANT PHYSIOLOGY 166.1 (2014): 152–167. Print.
APA
Hudik, E., Yoshioka, Y., Domenichini, S., Bourge, M., Soubigout-Taconnat, L., Mazubert, C., Yi, D., et al. (2014). Chloroplast dysfunction causes multiple defects in cell cycle progression in the Arabidopsis crumpled leaf mutant. PLANT PHYSIOLOGY, 166(1), 152–167.
Chicago author-date
Hudik, Elodie, Yasushi Yoshioka, Séverine Domenichini, Mickaël Bourge, Ludivine Soubigout-Taconnat, Christelle Mazubert, Dalong Yi, et al. 2014. “Chloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis Crumpled Leaf Mutant.” Plant Physiology 166 (1): 152–167.
Chicago author-date (all authors)
Hudik, Elodie, Yasushi Yoshioka, Séverine Domenichini, Mickaël Bourge, Ludivine Soubigout-Taconnat, Christelle Mazubert, Dalong Yi, Sandrine Bujaldon, Hiroyuki Hayashi, Lieven De Veylder, Catherine Bergounioux, Moussa Benhamed, and Cécile Raynaud. 2014. “Chloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis Crumpled Leaf Mutant.” Plant Physiology 166 (1): 152–167.
Vancouver
1.
Hudik E, Yoshioka Y, Domenichini S, Bourge M, Soubigout-Taconnat L, Mazubert C, et al. Chloroplast dysfunction causes multiple defects in cell cycle progression in the Arabidopsis crumpled leaf mutant. PLANT PHYSIOLOGY. 2014;166(1):152–67.
IEEE
[1]
E. Hudik et al., “Chloroplast dysfunction causes multiple defects in cell cycle progression in the Arabidopsis crumpled leaf mutant,” PLANT PHYSIOLOGY, vol. 166, no. 1, pp. 152–167, 2014.
@article{5722786,
  abstract     = {The majority of research on cell cycle regulation is focused on the nuclear events that govern the replication and segregation of the genome between the two daughter cells. However, eukaryotic cells contain several compartmentalized organelles with specialized functions, and coordination among these organelles is required for proper cell cycle progression, as evidenced by the isolation of several mutants in which both organelle function and overall plant development were affected. To investigate how chloroplast dysfunction affects the cell cycle, we analyzed the crumpled leaf (crl) mutant of Arabidopsis (Arabidopsis thaliana), which is deficient for a chloroplastic protein and displays particularly severe developmental defects. In the crl mutant, we reveal that cell cycle regulation is altered drastically and that meristematic cells prematurely enter differentiation, leading to reduced plant stature and early endoreduplication in the leaves. This response is due to the repression of several key cell cycle regulators as well as constitutive activation of stress-response genes, among them the cell cycle inhibitor SIAMESE-RELATED5. One unique feature of the crl mutant is that it produces aplastidic cells in several organs, including the root tip. By investigating the consequence of the absence of plastids on cell cycle progression, we showed that nuclear DNA replication occurs in aplastidic cells in the root tip, which opens future research prospects regarding the dialogue between plastids and the nucleus during cell cycle regulation in higher plants.},
  author       = {Hudik, Elodie and Yoshioka, Yasushi and Domenichini, Séverine and Bourge, Mickaël and Soubigout-Taconnat, Ludivine and Mazubert, Christelle and Yi, Dalong and Bujaldon, Sandrine and Hayashi, Hiroyuki and De Veylder, Lieven and Bergounioux, Catherine and Benhamed, Moussa and Raynaud, Cécile},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keywords     = {PLASTIDS,SIGNALS,PROLIFERATION,NUCLEAR GENE-EXPRESSION,DNA-REPLICATION,SINGLET OXYGEN,PLANT-CELL,STRESS RESPONSES,DIVISION,THALIANA},
  language     = {eng},
  number       = {1},
  pages        = {152--167},
  title        = {Chloroplast dysfunction causes multiple defects in cell cycle progression in the Arabidopsis crumpled leaf mutant},
  url          = {http://dx.doi.org/10.1104/pp.114.242628},
  volume       = {166},
  year         = {2014},
}

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