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Cell distribution and regenerative activity following meniscus replacement

Cathal J Moran, Selma Atmaca, Heidi Declercq UGent, Maria Cornelissen UGent and Peter Verdonk (2014) INTERNATIONAL ORTHOPAEDICS. 38(9). p.1937-1944
abstract
Meniscus replacement is of clinical benefit, but universal efficacy remains elusive. A greater understanding of the biological activity within implanted allografts or synthetic scaffolds may assist the development of improved surgical strategies. Biopsies of fresh-frozen allograft (n = 20), viable allograft (n = 18) and polyurethane scaffolds (n = 20) were obtained at second-look arthroscopy. Histological evaluation of tissue morphology and cell density/distribution was performed using haematoxylin-eosin (H&E) staining. Immunohistochemistry was used to detect the presence of CD34 (on progenitor cells and blood vessels) and smooth muscle actin (SMA)-positive structures and aggrecan. Collagen presence was investigated using picrosirius red staining. Cell density in the deep zone of the meniscus replacement was significantly higher in polyurethane scaffolds versus allograft transplants (p < 0.01) and also significantly higher in viable allograft compared with deep-frozen allograft (p < 0.01). CD34 staining was significantly higher in polyurethane and viable allografts versus deep-frozen allograft (progenitor cells p < 0.05; blood vessels p < 0.01). There were no significant differences in SMA or aggrecan staining across groups. All three specimen types demonstrated strong presence of collagen type I. Both viable allograft and a polyurethane meniscal scaffold show enhanced morphological, cell-distribution and regenerative patterns over deep-frozen allograft following surgical implantation. Given the limitations in viable allograft availability, these findings support the continued development of synthetic scaffolds for meniscus replacement surgery.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
LESIONS, ALLOGRAFTS, TRANSPLANTATION, REPAIR, OUTCOMES, POLYURETHANE SCAFFOLD, Regeneration, Histology, Allograft, Replacement, Meniscus
journal title
INTERNATIONAL ORTHOPAEDICS
Int. Orthop.
volume
38
issue
9
pages
1937 - 1944
Web of Science type
Article
Web of Science id
000341501400021
JCR category
ORTHOPEDICS
JCR impact factor
2.11 (2014)
JCR rank
22/72 (2014)
JCR quartile
2 (2014)
ISSN
0341-2695
DOI
10.1007/s00264-014-2426-7
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5721591
handle
http://hdl.handle.net/1854/LU-5721591
date created
2014-10-14 11:52:38
date last changed
2016-12-19 15:46:46
@article{5721591,
  abstract     = {Meniscus replacement is of clinical benefit, but universal efficacy remains elusive. A greater understanding of the biological activity within implanted allografts or synthetic scaffolds may assist the development of improved surgical strategies. 
Biopsies of fresh-frozen allograft (n = 20), viable allograft (n = 18) and polyurethane scaffolds (n = 20) were obtained at second-look arthroscopy. Histological evaluation of tissue morphology and cell density/distribution was performed using haematoxylin-eosin (H\&E) staining. Immunohistochemistry was used to detect the presence of CD34 (on progenitor cells and blood vessels) and smooth muscle actin (SMA)-positive structures and aggrecan. Collagen presence was investigated using picrosirius red staining. 
Cell density in the deep zone of the meniscus replacement was significantly higher in polyurethane scaffolds versus allograft transplants (p {\textlangle} 0.01) and also significantly higher in viable allograft compared with deep-frozen allograft (p {\textlangle} 0.01). CD34 staining was significantly higher in polyurethane and viable allografts versus deep-frozen allograft (progenitor cells p {\textlangle} 0.05; blood vessels p {\textlangle} 0.01). There were no significant differences in SMA or aggrecan staining across groups. All three specimen types demonstrated strong presence of collagen type I. 
Both viable allograft and a polyurethane meniscal scaffold show enhanced morphological, cell-distribution and regenerative patterns over deep-frozen allograft following surgical implantation. Given the limitations in viable allograft availability, these findings support the continued development of synthetic scaffolds for meniscus replacement surgery.},
  author       = {Moran, Cathal J and Atmaca, Selma and Declercq, Heidi and Cornelissen, Maria and Verdonk, Peter},
  issn         = {0341-2695},
  journal      = {INTERNATIONAL ORTHOPAEDICS},
  keyword      = {LESIONS,ALLOGRAFTS,TRANSPLANTATION,REPAIR,OUTCOMES,POLYURETHANE SCAFFOLD,Regeneration,Histology,Allograft,Replacement,Meniscus},
  language     = {eng},
  number       = {9},
  pages        = {1937--1944},
  title        = {Cell distribution and regenerative activity following meniscus replacement},
  url          = {http://dx.doi.org/10.1007/s00264-014-2426-7},
  volume       = {38},
  year         = {2014},
}

Chicago
Moran, Cathal J, Selma Atmaca, Heidi Declercq, Maria Cornelissen, and Peter Verdonk. 2014. “Cell Distribution and Regenerative Activity Following Meniscus Replacement.” International Orthopaedics 38 (9): 1937–1944.
APA
Moran, C. J., Atmaca, S., Declercq, H., Cornelissen, M., & Verdonk, P. (2014). Cell distribution and regenerative activity following meniscus replacement. INTERNATIONAL ORTHOPAEDICS, 38(9), 1937–1944.
Vancouver
1.
Moran CJ, Atmaca S, Declercq H, Cornelissen M, Verdonk P. Cell distribution and regenerative activity following meniscus replacement. INTERNATIONAL ORTHOPAEDICS. 2014;38(9):1937–44.
MLA
Moran, Cathal J, Selma Atmaca, Heidi Declercq, et al. “Cell Distribution and Regenerative Activity Following Meniscus Replacement.” INTERNATIONAL ORTHOPAEDICS 38.9 (2014): 1937–1944. Print.