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High throughput micro-well generation of hepatocyte micro-aggregates for tissue engineering

(2014) PLOS ONE. 9(8).
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Abstract
The main challenge in hepatic tissue engineering is the fast dedifferentiation of primary hepatocytes in vitro. One successful approach to maintain hepatocyte phenotype on the longer term is the cultivation of cells as aggregates. This paper demonstrates the use of an agarose micro-well chip for the high throughput generation of hepatocyte aggregates, uniform in size. In our study we observed that aggregation of hepatocytes had a beneficial effect on the expression of certain hepatocyte specific markers. Moreover we observed that the beneficial effect was dependent on the aggregate dimensions, indicating that aggregate parameters should be carefully considered. In a second part of the study, the selected aggregates were immobilized by encapsulation in methacrylamide-modified gelatin. Phenotype evaluations revealed that a stable hepatocyte phenotype could be maintained during 21 days when encapsulated in the hydrogel. In conclusion we have demonstrated the beneficial use of micro-well chips for hepatocyte aggregation and the size-dependent effects on hepatocyte phenotype. We also pointed out that methacrylamide-modified gelatin is suitable for the encapsulation of these aggregates.
Keywords
CELL-LADEN MICROGELS, E-CADHERIN, SPHEROIDS, LIVER, SIZE, CONSTRUCTS, CULTURES, VIABILITY

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Citation

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MLA
Gevaert, Elien et al. “High Throughput Micro-well Generation of Hepatocyte Micro-aggregates for Tissue Engineering.” PLOS ONE 9.8 (2014): n. pag. Print.
APA
Gevaert, E., Dollé, L., Billiet, T., Dubruel, P., van Grunsven, L., van Appeldoorn, A., & Cornelissen, M. (2014). High throughput micro-well generation of hepatocyte micro-aggregates for tissue engineering. PLOS ONE, 9(8).
Chicago author-date
Gevaert, Elien, Laurent Dollé, Thomas Billiet, Peter Dubruel, Leo van Grunsven, Aart van Appeldoorn, and Maria Cornelissen. 2014. “High Throughput Micro-well Generation of Hepatocyte Micro-aggregates for Tissue Engineering.” Plos One 9 (8).
Chicago author-date (all authors)
Gevaert, Elien, Laurent Dollé, Thomas Billiet, Peter Dubruel, Leo van Grunsven, Aart van Appeldoorn, and Maria Cornelissen. 2014. “High Throughput Micro-well Generation of Hepatocyte Micro-aggregates for Tissue Engineering.” Plos One 9 (8).
Vancouver
1.
Gevaert E, Dollé L, Billiet T, Dubruel P, van Grunsven L, van Appeldoorn A, et al. High throughput micro-well generation of hepatocyte micro-aggregates for tissue engineering. PLOS ONE. 2014;9(8).
IEEE
[1]
E. Gevaert et al., “High throughput micro-well generation of hepatocyte micro-aggregates for tissue engineering,” PLOS ONE, vol. 9, no. 8, 2014.
@article{5721564,
  abstract     = {{The main challenge in hepatic tissue engineering is the fast dedifferentiation of primary hepatocytes in vitro. One successful approach to maintain hepatocyte phenotype on the longer term is the cultivation of cells as aggregates. This paper demonstrates the use of an agarose micro-well chip for the high throughput generation of hepatocyte aggregates, uniform in size. In our study we observed that aggregation of hepatocytes had a beneficial effect on the expression of certain hepatocyte specific markers. Moreover we observed that the beneficial effect was dependent on the aggregate dimensions, indicating that aggregate parameters should be carefully considered. In a second part of the study, the selected aggregates were immobilized by encapsulation in methacrylamide-modified gelatin. Phenotype evaluations revealed that a stable hepatocyte phenotype could be maintained during 21 days when encapsulated in the hydrogel. In conclusion we have demonstrated the beneficial use of micro-well chips for hepatocyte aggregation and the size-dependent effects on hepatocyte phenotype. We also pointed out that methacrylamide-modified gelatin is suitable for the encapsulation of these aggregates.}},
  articleno    = {{e105171}},
  author       = {{Gevaert, Elien and Dollé, Laurent and Billiet, Thomas and Dubruel, Peter and van Grunsven, Leo and van Appeldoorn, Aart and Cornelissen, Maria}},
  issn         = {{1932-6203}},
  journal      = {{PLOS ONE}},
  keywords     = {{CELL-LADEN MICROGELS,E-CADHERIN,SPHEROIDS,LIVER,SIZE,CONSTRUCTS,CULTURES,VIABILITY}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{9}},
  title        = {{High throughput micro-well generation of hepatocyte micro-aggregates for tissue engineering}},
  url          = {{http://dx.doi.org/10.1371/journal.pone.0105171}},
  volume       = {{9}},
  year         = {{2014}},
}

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