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Equine oviduct explant culture: a basic model to decipher embryo-maternal communication

Hilde Nelis (UGent) , Katharina D'Herde (UGent) , Karen Goossens, Lynn Vandenberghe (UGent) , Bart Leemans (UGent) , Katrien Forier (UGent) , Katrien Smits (UGent) , Kevin Braeckmans (UGent) , Luc Peelman (UGent) and Ann Van Soom (UGent)
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Project
NB-Photonics
Abstract
Equine embryos remain for 6 days in the oviduct and thus there is a need for an in vitro model to study embryo-oviductal interactions in the horse, since this subtle way of communication is very difficult to analyse in vivo. Until now, no equine oviduct explant culture model has been characterised both morphologically and functionally. Therefore, we established a culture system for equine oviduct explants that maintained epithelial morphology during 6 days of culture, as revealed by light microscopy and transmission electron microscopy. We demonstrated the presence of highly differentiated, tall columnar, pseudostratified epithelium with basal nuclei, numerous nucleoli, secretory granules and apical cilia, which is very similar to the in vivo situation. Both epithelium and stromal cells originating from the lamina propria are represented in the explants. Moreover, at least 98% of the cells remained membrane intact and fewer than 2% of the cells were apoptotic after 6 days of culture. Although dark-cell degeneration, which is a hypoxia-related type of cell death, was observed in the centre of the explants, quantitative real-time PCR failed to detect upregulation of the hypoxia-related marker genes HIF1A, VEGFA, uPA, GLUT1 and PAI1. Since the explants remained morphologically and functionally intact and since the system is easy to set up, it appears to be an excellent tool for proteome, transcriptome and miRNome analysis in order to unravel embryo-maternal interactions in the horse.
Keywords
DEVELOPMENTALLY IMPORTANT GENES, horse, GROWTH-FACTOR EXPRESSION, IN-VITRO PRODUCTION, REAL-TIME PCR, BOVINE OVIDUCT, EPITHELIAL-CELLS, PREIMPLANTATION EMBRYOS, CONDITIONED MEDIUM, BLASTOCYST STAGE, ACTIVE CASPASE-3, dark-cell degeneration

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Citation

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Chicago
Nelis, Hilde, Katharina D’Herde, Karen Goossens, Lynn Vandenberghe, Bart Leemans, Katrien Forier, Katrien Smits, Kevin Braeckmans, Luc Peelman, and Ann Van Soom. 2014. “Equine Oviduct Explant Culture: a Basic Model to Decipher Embryo-maternal Communication.” Reproduction Fertility and Development 26 (7): 954–966.
APA
Nelis, Hilde, D’Herde, K., Goossens, K., Vandenberghe, L., Leemans, B., Forier, K., Smits, K., et al. (2014). Equine oviduct explant culture: a basic model to decipher embryo-maternal communication. REPRODUCTION FERTILITY AND DEVELOPMENT, 26(7), 954–966.
Vancouver
1.
Nelis H, D’Herde K, Goossens K, Vandenberghe L, Leemans B, Forier K, et al. Equine oviduct explant culture: a basic model to decipher embryo-maternal communication. REPRODUCTION FERTILITY AND DEVELOPMENT. 2014;26(7):954–66.
MLA
Nelis, Hilde, Katharina D’Herde, Karen Goossens, et al. “Equine Oviduct Explant Culture: a Basic Model to Decipher Embryo-maternal Communication.” REPRODUCTION FERTILITY AND DEVELOPMENT 26.7 (2014): 954–966. Print.
@article{5719553,
  abstract     = {Equine embryos remain for 6 days in the oviduct and thus there is a need for an in vitro model to study embryo-oviductal interactions in the horse, since this subtle way of communication is very difficult to analyse in vivo. Until now, no equine oviduct explant culture model has been characterised both morphologically and functionally. Therefore, we established a culture system for equine oviduct explants that maintained epithelial morphology during 6 days of culture, as revealed by light microscopy and transmission electron microscopy. We demonstrated the presence of highly differentiated, tall columnar, pseudostratified epithelium with basal nuclei, numerous nucleoli, secretory granules and apical cilia, which is very similar to the in vivo situation. Both epithelium and stromal cells originating from the lamina propria are represented in the explants. Moreover, at least 98\% of the cells remained membrane intact and fewer than 2\% of the cells were apoptotic after 6 days of culture. Although dark-cell degeneration, which is a hypoxia-related type of cell death, was observed in the centre of the explants, quantitative real-time PCR failed to detect upregulation of the hypoxia-related marker genes HIF1A, VEGFA, uPA, GLUT1 and PAI1. Since the explants remained morphologically and functionally intact and since the system is easy to set up, it appears to be an excellent tool for proteome, transcriptome and miRNome analysis in order to unravel embryo-maternal interactions in the horse.},
  author       = {Nelis, Hilde and D'Herde, Katharina and Goossens, Karen and Vandenberghe, Lynn and Leemans, Bart and Forier, Katrien and Smits, Katrien and Braeckmans, Kevin and Peelman, Luc and Van Soom, Ann},
  issn         = {1031-3613},
  journal      = {REPRODUCTION FERTILITY AND DEVELOPMENT},
  keyword      = {DEVELOPMENTALLY IMPORTANT GENES,horse,GROWTH-FACTOR EXPRESSION,IN-VITRO PRODUCTION,REAL-TIME PCR,BOVINE OVIDUCT,EPITHELIAL-CELLS,PREIMPLANTATION EMBRYOS,CONDITIONED MEDIUM,BLASTOCYST STAGE,ACTIVE CASPASE-3,dark-cell degeneration},
  language     = {eng},
  number       = {7},
  pages        = {954--966},
  title        = {Equine oviduct explant culture: a basic model to decipher embryo-maternal communication},
  url          = {http://dx.doi.org/10.1071/RD13089},
  volume       = {26},
  year         = {2014},
}

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