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Helicobacter ailurogastricus sp. nov.: a 'light' version of Helicobacter heilmannii (sensu stricto)?

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Abstract
H. heilmannii (sensu stricto) is a zoonotic bacterium that naturally colonizes the stomach of cats and dogs. It has been associated with gastric disease in humans. We isolated 9 strains of Helicobacter from the gastric mucosa of cats and classified them as H. heilmannii based on ureAB and 16S rRNA gene sequences. In a previous experimental infection study, using a Mongolian gerbil model, variation in colonization capacity and virulence was demonstrated for these 9 strains. Five out of the 9 strains were shown to be highly virulent, whereas the remaining 4 strains were clearly less virulent. In vitro binding assays showed that the same 4 strains had lower binding capacity to human gastric mucins and epithelial cells. To determine if the differences in colonization and virulence were due to the presence of specific virulence factors, whole genome sequencing was applied to all 9 strains. DNA-DNA hybridization (DDH) parameters, estimated in silico by calculating whole-genome distances using the Genome to Genome Distance Calculator tool, yielded a probability via logistic regression of 96.30% that the 5 highly virulent strains, including the H. heilmannii reference strain, belong to the same species. They were hence identified as H. heilmannii. The DDH estimates for the 4 less virulent strains indicated a probability of only 0.01% that these strains belong to the H. heilmannii species. These data support their re-classification as a novel species for which the name H. ailurogastricus sp. nov (of a cat’s stomach) is proposed. Genomic analyses showed that H. heilmannii harbors some outer membrane proteins that are not present in H. ailurogastricus, but their role in the colonization process is so far unknown. In addition, the Helicobacter iceA gene, which is putatively involved in ulcer formation, was absent in H. ailurogastricus but present in H. heilmannii.

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Chicago
Joosten, Myrthe, Mirko Rossi, Alfred Tay, Emma Skoog, Medea Padra, Fanny Thirriot, Tim Perkins, et al. 2014. “Helicobacter Ailurogastricus Sp. Nov.: a ‘Light’ Version of Helicobacter Heilmannii (sensu Stricto)?” In Pathogenesis and Host Response in Helicobacter Infections, 11th International Workshop, Abstracts. European Helicobacter Study Group (EHSG).
APA
Joosten, M., Rossi, M., Tay, A., Skoog, E., Padra, M., Thirriot, F., Perkins, T., et al. (2014). Helicobacter ailurogastricus sp. nov.: a “light” version of Helicobacter heilmannii (sensu stricto)? Pathogenesis and Host Response in Helicobacter Infections, 11th International workshop, Abstracts. Presented at the 11th International workshop on Pathogenesis and Host Response in Helicobacter Infections, European Helicobacter Study Group (EHSG).
Vancouver
1.
Joosten M, Rossi M, Tay A, Skoog E, Padra M, Thirriot F, et al. Helicobacter ailurogastricus sp. nov.: a “light” version of Helicobacter heilmannii (sensu stricto)? Pathogenesis and Host Response in Helicobacter Infections, 11th International workshop, Abstracts. European Helicobacter Study Group (EHSG); 2014.
MLA
Joosten, Myrthe, Mirko Rossi, Alfred Tay, et al. “Helicobacter Ailurogastricus Sp. Nov.: a ‘Light’ Version of Helicobacter Heilmannii (sensu Stricto)?” Pathogenesis and Host Response in Helicobacter Infections, 11th International Workshop, Abstracts. European Helicobacter Study Group (EHSG), 2014. Print.
@inproceedings{5718831,
  abstract     = {H. heilmannii (sensu stricto) is a zoonotic bacterium that naturally colonizes the stomach of cats and dogs. It has been associated with gastric disease in humans. We isolated 9 strains of Helicobacter from the gastric mucosa of cats and classified them as H. heilmannii based on ureAB and 16S rRNA gene sequences. In a previous experimental infection study, using a Mongolian gerbil model, variation in colonization capacity and virulence was demonstrated for these 9 strains. Five out of the 9 strains were shown to be highly virulent, whereas the  remaining 4 strains were clearly less virulent. In vitro binding assays showed that the same 4 strains had lower binding capacity to human gastric mucins and epithelial cells. To determine if the differences in colonization and virulence were due to the presence of specific virulence factors, whole genome sequencing was applied to all 9 strains. DNA-DNA hybridization (DDH) parameters, estimated in silico by calculating whole-genome distances using the Genome to Genome Distance Calculator tool, yielded a probability via logistic regression of 96.30\% that the 5 highly virulent strains, including the H. heilmannii reference strain, belong to the same species. They were hence identified as H. heilmannii. The DDH estimates for the 4 less virulent strains indicated a probability of only 0.01\% that these strains belong to the H. heilmannii species. These data support their re-classification as a novel species for which the name H. ailurogastricus sp. nov (of a cat{\textquoteright}s stomach) is proposed. Genomic analyses showed that H. heilmannii harbors some outer membrane proteins that are not present in H. ailurogastricus, but their role in the colonization process is so far unknown. In addition, the Helicobacter iceA gene, which is putatively involved in ulcer formation, was absent in H. ailurogastricus but present in H. heilmannii.},
  articleno    = {abstract HP-14},
  author       = {Joosten, Myrthe and Rossi, Mirko and Tay, Alfred and Skoog, Emma and Padra, Medea and Thirriot, Fanny and Perkins, Tim and Flahou, Bram and Ducatelle, Richard and Linden, Sara and Haesebrouck, Freddy and Smet, Annemieke},
  booktitle    = {Pathogenesis and Host Response in Helicobacter Infections, 11th International workshop, Abstracts},
  language     = {eng},
  location     = {Helsingor, Denmark},
  publisher    = {European Helicobacter Study Group (EHSG)},
  title        = {Helicobacter ailurogastricus sp. nov.: a 'light' version of Helicobacter heilmannii (sensu stricto)?},
  year         = {2014},
}