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The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens

Gunther Antonissen (UGent) , Filip Van Immerseel (UGent) , Frank Pasmans (UGent) , Richard Ducatelle (UGent) , Freddy Haesebrouck (UGent) , Leen Timbermont (UGent) , Marc Verlinden (UGent) , Geert Janssens (UGent) , Venessa Eeckhaut (UGent) , Mia Eeckhout (UGent) , et al.
(2014) PLOS ONE. 9(9).
Author
Organization
Abstract
Both mycotoxin contamination of feed and Clostridium perfringens-induced necrotic enteritis have an increasing global economic impact on poultry production. Especially the Fusarium mycotoxin deoxynivalenol (DON) is a common feed contaminant. This study aimed at examining the predisposing effect of DON on the development of necrotic enteritis in broiler chickens. An experimental Clostridium perfringens infection study revealed that DON, at a contamination level of 3,000 to 4,000 mg/kg feed, increased the percentage of birds with subclinical necrotic enteritis from 2062.6% to 4763.0% (P<0.001). DON significantly reduced the transepithelial electrical resistance in duodenal segments (P<0.001) and decreased duodenal villus height (P = 0.014) indicating intestinal barrier disruption and intestinal epithelial damage, respectively. This may lead to an increased permeability of the intestinal epithelium and decreased absorption of dietary proteins. Protein analysis of duodenal content indeed showed that DON contamination resulted in a significant increase in total protein concentration (P = 0.023). Furthermore, DON had no effect on in vitro growth, alpha toxin production and netB toxin transcription of Clostridium perfringens. In conclusion, feed contamination with DON at concentrations below the European maximum guidance level of 5,000 mg/kg feed, is a predisposing factor for the development of necrotic enteritis in broilers. These results are associated with a negative effect of DON on the intestinal barrier function and increased intestinal protein availability, which may stimulate growth and toxin production of Clostridium perfringens.
Keywords
TOXIN, LAYING HENS, MODEL, GUT, PATHOGENESIS, PERFORMANCE, ABSORPTION, PARAMETERS, EPITHELIUM, POULTRY

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MLA
Antonissen, Gunther et al. “The Mycotoxin Deoxynivalenol Predisposes for the Development of Clostridium Perfringens-induced Necrotic Enteritis in Broiler Chickens.” PLOS ONE 9.9 (2014): n. pag. Print.
APA
Antonissen, G., Van Immerseel, F., Pasmans, F., Ducatelle, R., Haesebrouck, F., Timbermont, L., Verlinden, M., et al. (2014). The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens. PLOS ONE, 9(9).
Chicago author-date
Antonissen, Gunther, Filip Van Immerseel, Frank Pasmans, Richard Ducatelle, Freddy Haesebrouck, Leen Timbermont, Marc Verlinden, et al. 2014. “The Mycotoxin Deoxynivalenol Predisposes for the Development of Clostridium Perfringens-induced Necrotic Enteritis in Broiler Chickens.” Plos One 9 (9).
Chicago author-date (all authors)
Antonissen, Gunther, Filip Van Immerseel, Frank Pasmans, Richard Ducatelle, Freddy Haesebrouck, Leen Timbermont, Marc Verlinden, Geert Janssens, Venessa Eeckhaut, Mia Eeckhout, Sarah De Saeger, Sabine Hessenberger, An Martel, and Siska Croubels. 2014. “The Mycotoxin Deoxynivalenol Predisposes for the Development of Clostridium Perfringens-induced Necrotic Enteritis in Broiler Chickens.” Plos One 9 (9).
Vancouver
1.
Antonissen G, Van Immerseel F, Pasmans F, Ducatelle R, Haesebrouck F, Timbermont L, et al. The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens. PLOS ONE. 2014;9(9).
IEEE
[1]
G. Antonissen et al., “The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens,” PLOS ONE, vol. 9, no. 9, 2014.
@article{5717989,
  abstract     = {Both mycotoxin contamination of feed and Clostridium perfringens-induced necrotic enteritis have an increasing global economic impact on poultry production. Especially the Fusarium mycotoxin deoxynivalenol (DON) is a common feed contaminant. This study aimed at examining the predisposing effect of DON on the development of necrotic enteritis in broiler chickens. An experimental Clostridium perfringens infection study revealed that DON, at a contamination level of 3,000 to 4,000 mg/kg feed, increased the percentage of birds with subclinical necrotic enteritis from 2062.6% to 4763.0% (P<0.001). DON significantly reduced the transepithelial electrical resistance in duodenal segments (P<0.001) and decreased duodenal villus height (P = 0.014) indicating intestinal barrier disruption and intestinal epithelial damage, respectively. This may lead to an increased permeability of the intestinal epithelium and decreased absorption of dietary proteins. Protein analysis of duodenal content indeed showed that DON contamination resulted in a significant increase in total protein concentration (P = 0.023). Furthermore, DON had no effect on in vitro growth, alpha toxin production and netB toxin transcription of Clostridium perfringens. In conclusion, feed contamination with DON at concentrations below the European maximum guidance level of 5,000 mg/kg feed, is a predisposing factor for the development of necrotic enteritis in broilers. These results are associated with a negative effect of DON on the intestinal barrier function and increased intestinal protein availability, which may stimulate growth and toxin production of Clostridium perfringens.},
  articleno    = {e108775},
  author       = {Antonissen, Gunther and Van Immerseel, Filip and Pasmans, Frank and Ducatelle, Richard and Haesebrouck, Freddy and Timbermont, Leen and Verlinden, Marc and Janssens, Geert and Eeckhaut, Venessa and Eeckhout, Mia and De Saeger, Sarah and Hessenberger, Sabine and Martel, An and Croubels, Siska},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {TOXIN,LAYING HENS,MODEL,GUT,PATHOGENESIS,PERFORMANCE,ABSORPTION,PARAMETERS,EPITHELIUM,POULTRY},
  language     = {eng},
  number       = {9},
  pages        = {8},
  title        = {The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens},
  url          = {http://dx.doi.org/10.1371/journal.pone.0108775},
  volume       = {9},
  year         = {2014},
}

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