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What is stress?: dose-response effects in commonly used in vitro stress assays

Hannes Claeys, Sofie Van Landeghem, Marieke Dubois, Katrien Maleux UGent and Dirk Inzé UGent (2014) PLANT PHYSIOLOGY. 165(2). p.519-527
abstract
In vitro stress assays are commonly used to study the responses of plants to abiotic stress and to assess stress tolerance. A literature review reveals that most studies use very high stress levels and measure criteria such as germination, plant survival, or the development of visual symptoms such as bleaching. However, we show that these parameters are indicators of very severe stress, and such studies thus only provide incomplete information about stress sensitivity in Arabidopsis (Arabidopsis thaliana). Similarly, transcript analysis revealed that typical stress markers are only induced at high stress levels in young seedlings. Therefore, tools are needed to study the effects of mild stress. We found that the commonly used stress-inducing agents mannitol, sorbitol, NaCl, and hydrogen peroxide impact shoot growth in a highly specific and dose-dependent way. Therefore, shoot growth is a sensitive, relevant, and easily measured phenotype to assess stress tolerance over a wide range of stress levels. Finally, our data suggest that care should be taken when using mannitol as an osmoticum.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CROSS-TALK, DATA SET, LEAF GROWTH, COLD STRESS, RAPID GROWTH, OSMOTIC-STRESS, DROUGHT TOLERANCE, WATER-LIMITING CONDITIONS, ARABIDOPSIS-THALIANA, MODEL DATA-ANALYSIS
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
165
issue
2
pages
519 - 527
Web of Science type
Article
Web of Science id
000337242700004
JCR category
PLANT SCIENCES
JCR impact factor
6.841 (2014)
JCR rank
8/204 (2014)
JCR quartile
1 (2014)
ISSN
0032-0889
DOI
10.1104/pp.113.234641
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
5714750
handle
http://hdl.handle.net/1854/LU-5714750
date created
2014-10-01 13:20:31
date last changed
2016-12-19 15:46:46
@article{5714750,
  abstract     = {In vitro stress assays are commonly used to study the responses of plants to abiotic stress and to assess stress tolerance. A literature review reveals that most studies use very high stress levels and measure criteria such as germination, plant survival, or the development of visual symptoms such as bleaching. However, we show that these parameters are indicators of very severe stress, and such studies thus only provide incomplete information about stress sensitivity in Arabidopsis (Arabidopsis thaliana). Similarly, transcript analysis revealed that typical stress markers are only induced at high stress levels in young seedlings. Therefore, tools are needed to study the effects of mild stress. We found that the commonly used stress-inducing agents mannitol, sorbitol, NaCl, and hydrogen peroxide impact shoot growth in a highly specific and dose-dependent way. Therefore, shoot growth is a sensitive, relevant, and easily measured phenotype to assess stress tolerance over a wide range of stress levels. Finally, our data suggest that care should be taken when using mannitol as an osmoticum.},
  author       = {Claeys, Hannes and Van Landeghem, Sofie and Dubois, Marieke and Maleux, Katrien and Inz{\'e}, Dirk},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {CROSS-TALK,DATA SET,LEAF GROWTH,COLD STRESS,RAPID GROWTH,OSMOTIC-STRESS,DROUGHT TOLERANCE,WATER-LIMITING CONDITIONS,ARABIDOPSIS-THALIANA,MODEL DATA-ANALYSIS},
  language     = {eng},
  number       = {2},
  pages        = {519--527},
  title        = {What is stress?: dose-response effects in commonly used in vitro stress assays},
  url          = {http://dx.doi.org/10.1104/pp.113.234641},
  volume       = {165},
  year         = {2014},
}

Chicago
Claeys, Hannes, Sofie Van Landeghem, Marieke Dubois, Katrien Maleux, and Dirk Inzé. 2014. “What Is Stress?: Dose-response Effects in Commonly Used in Vitro Stress Assays.” Plant Physiology 165 (2): 519–527.
APA
Claeys, H., Van Landeghem, S., Dubois, M., Maleux, K., & Inzé, D. (2014). What is stress?: dose-response effects in commonly used in vitro stress assays. PLANT PHYSIOLOGY, 165(2), 519–527.
Vancouver
1.
Claeys H, Van Landeghem S, Dubois M, Maleux K, Inzé D. What is stress?: dose-response effects in commonly used in vitro stress assays. PLANT PHYSIOLOGY. 2014;165(2):519–27.
MLA
Claeys, Hannes, Sofie Van Landeghem, Marieke Dubois, et al. “What Is Stress?: Dose-response Effects in Commonly Used in Vitro Stress Assays.” PLANT PHYSIOLOGY 165.2 (2014): 519–527. Print.