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Demonstration of microchimerism in pregnant sows and effects of congenital PRRSV infection

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Abstract
The presence of foreign cells within the tissue/circulation of an individual is described as microchimerism. The main purpose of the present investigation was to study if microchimerism occurs in healthy sows/fetuses and if porcine reproductive and respiratory syndrome virus (PRRSV) infection influences this phenomenon. Six dams were inoculated intranasally with PRRSV and three non-inoculated dams served as controls. Male DNA was detected in female fetal sera of all dams via PCR. Male DNA was also detected in the maternal circulation. Sex-typing FISH showed the presence of male cells in the female fetal organs and vice versa. PRRSV infection did not influence microchimerism, but might misuse maternal and sibling microchimeric cells to enter fetuses.
Keywords
PATHOGENESIS, FETUSES, PERIPHERAL-BLOOD, IDENTIFICATION, IN-UTERO, MATERNAL CELLS, UMBILICAL-CORD BLOOD, POLYMERASE CHAIN-REACTION, NONINVASIVE PRENATAL-DIAGNOSIS, RESPIRATORY SYNDROME VIRUS

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Chicago
Karniychuk, Uladzimir, Wander Van Breedam, Nadine Van Roy, Claire Rogel-Gaillard, and Hans Nauwynck. 2012. “Demonstration of Microchimerism in Pregnant Sows and Effects of Congenital PRRSV Infection.” Veterinary Research 43.
APA
Karniychuk, U., Van Breedam, W., Van Roy, N., Rogel-Gaillard, C., & Nauwynck, H. (2012). Demonstration of microchimerism in pregnant sows and effects of congenital PRRSV infection. VETERINARY RESEARCH, 43.
Vancouver
1.
Karniychuk U, Van Breedam W, Van Roy N, Rogel-Gaillard C, Nauwynck H. Demonstration of microchimerism in pregnant sows and effects of congenital PRRSV infection. VETERINARY RESEARCH. 2012;43.
MLA
Karniychuk, Uladzimir, Wander Van Breedam, Nadine Van Roy, et al. “Demonstration of Microchimerism in Pregnant Sows and Effects of Congenital PRRSV Infection.” VETERINARY RESEARCH 43 (2012): n. pag. Print.
@article{5710142,
  abstract     = {The presence of foreign cells within the tissue/circulation of an individual is described as microchimerism. The main purpose of the present investigation was to study if microchimerism occurs in healthy sows/fetuses and if porcine reproductive and respiratory syndrome virus (PRRSV) infection influences this phenomenon. Six dams were inoculated intranasally with PRRSV and three non-inoculated dams served as controls. Male DNA was detected in female fetal sera of all dams via PCR. Male DNA was also detected in the maternal circulation. Sex-typing FISH showed the presence of male cells in the female fetal organs and vice versa. PRRSV infection did not influence microchimerism, but might misuse maternal and sibling microchimeric cells to enter fetuses.},
  articleno    = {19},
  author       = {Karniychuk, Uladzimir and Van Breedam, Wander and Van Roy, Nadine and Rogel-Gaillard, Claire and Nauwynck, Hans},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  language     = {eng},
  pages        = {7},
  title        = {Demonstration of microchimerism in pregnant sows and effects of congenital PRRSV infection},
  url          = {http://dx.doi.org/10.1186/1297-9716-43-19},
  volume       = {43},
  year         = {2012},
}

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