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Detection of total and PRRSV-specific antibodies in oral fluids collected with different rope types from PRRSV-vaccinated and experimentally infected pigs

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Abstract
Background: Oral fluid collected by means of ropes has the potential to replace serum for monitoring and surveillance of important swine pathogens. Until now, the most commonly used method to collect oral fluid is by hanging a cotton rope in a pen. However, concerns about the influence of rope material on subsequent immunological assays have been raised. In this study, we evaluated six different rope materials for the collection of oral fluid and the subsequent detection of total and PRRSV-specific antibodies of different isotypes in oral fluid collected from PRRSV-vaccinated and infected pigs. Results: An initial experiment showed that IgA is the predominant antibody isotype in porcine saliva. Moreover, it was found that synthetic ropes may yield higher amounts of IgA, whereas all rope types seemed to be equally suitable for IgG collection. Although IgA is the predominant antibody isotype in porcine oral fluid, the PRRSV-specific IgA-based IPMA and ELISA tests were clearly not ideal for sensitive detection of PRRSV-specific IgA antibodies. In contrast, PRRSV-specific IgG in oral fluids was readily detected in PRRSV-specific IgG-based IPMA and ELISA tests, indicating that IgG is a more reliable isotype for monitoring PRRSV-specific antibody immunity in vaccinated/infected animals via oral fluids with the currently available tests. Conclusions: Since PRRSV-specific IgG detection seems more reliable than PRRSV-specific IgA detection for monitoring PRRSV-specific antibody immunity via oral fluids, and since all rope types yield equal amounts of IgG, it seems that the currently used cotton ropes are an appropriate choice for sample collection in PRRSV monitoring.
Keywords
PRRSV, RESPIRATORY-SYNDROME-VIRUS, Oral fluid, Collection method, SALIVARY BIOMARKERS, Antibody detection, SERUM, IGA, SAMPLES, IMMUNITY, SURVEILLANCE, IMMUNOASSAY, SPECIMENS, DIAGNOSIS

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  • 10.1186 1746-6148-10-134
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Chicago
Decorte, Inge, Wander Van Breedam, Yves Van der Stede, Hans Nauwynck, Nick De Regge, and Ann Brigitte Cay. 2014. “Detection of Total and PRRSV-specific Antibodies in Oral Fluids Collected with Different Rope Types from PRRSV-vaccinated and Experimentally Infected Pigs.” Bmc Veterinary Research 10.
APA
Decorte, I., Van Breedam, W., Van der Stede, Y., Nauwynck, H., De Regge, N., & Cay, A. B. (2014). Detection of total and PRRSV-specific antibodies in oral fluids collected with different rope types from PRRSV-vaccinated and experimentally infected pigs. BMC VETERINARY RESEARCH, 10.
Vancouver
1.
Decorte I, Van Breedam W, Van der Stede Y, Nauwynck H, De Regge N, Cay AB. Detection of total and PRRSV-specific antibodies in oral fluids collected with different rope types from PRRSV-vaccinated and experimentally infected pigs. BMC VETERINARY RESEARCH. 2014;10.
MLA
Decorte, Inge, Wander Van Breedam, Yves Van der Stede, et al. “Detection of Total and PRRSV-specific Antibodies in Oral Fluids Collected with Different Rope Types from PRRSV-vaccinated and Experimentally Infected Pigs.” BMC VETERINARY RESEARCH 10 (2014): n. pag. Print.
@article{5710065,
  abstract     = {Background: Oral fluid collected by means of ropes has the potential to replace serum for monitoring and surveillance of important swine pathogens. Until now, the most commonly used method to collect oral fluid is by hanging a cotton rope in a pen. However, concerns about the influence of rope material on subsequent immunological assays have been raised. In this study, we evaluated six different rope materials for the collection of oral fluid and the subsequent detection of total and PRRSV-specific antibodies of different isotypes in oral fluid collected from PRRSV-vaccinated and infected pigs.
Results: An initial experiment showed that IgA is the predominant antibody isotype in porcine saliva. Moreover, it was found that synthetic ropes may yield higher amounts of IgA, whereas all rope types seemed to be equally suitable for IgG collection. Although IgA is the predominant antibody isotype in porcine oral fluid, the PRRSV-specific IgA-based IPMA and ELISA tests were clearly not ideal for sensitive detection of PRRSV-specific IgA antibodies. In contrast, PRRSV-specific IgG in oral fluids was readily detected in PRRSV-specific IgG-based IPMA and ELISA tests, indicating that IgG is a more reliable isotype for monitoring PRRSV-specific antibody immunity in vaccinated/infected animals via oral fluids with the currently available tests.
Conclusions: Since PRRSV-specific IgG detection seems more reliable than PRRSV-specific IgA detection for monitoring PRRSV-specific antibody immunity via oral fluids, and since all rope types yield equal amounts of IgG, it seems that the currently used cotton ropes are an appropriate choice for sample collection in PRRSV monitoring.},
  articleno    = {134},
  author       = {Decorte, Inge and Van Breedam, Wander and Van der Stede, Yves and Nauwynck, Hans and De Regge, Nick and Cay, Ann Brigitte},
  issn         = {1746-6148},
  journal      = {BMC VETERINARY RESEARCH},
  keyword      = {PRRSV,RESPIRATORY-SYNDROME-VIRUS,Oral fluid,Collection method,SALIVARY BIOMARKERS,Antibody detection,SERUM,IGA,SAMPLES,IMMUNITY,SURVEILLANCE,IMMUNOASSAY,SPECIMENS,DIAGNOSIS},
  language     = {eng},
  pages        = {11},
  title        = {Detection of total and PRRSV-specific antibodies in oral fluids collected with different rope types from PRRSV-vaccinated and experimentally infected pigs},
  url          = {http://dx.doi.org/10.1186/1746-6148-10-134},
  volume       = {10},
  year         = {2014},
}

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