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Sulfenome mining in Arabidopsis thaliana

Cezary Waszczak, Mosammat Salma Akter, Dominique Eeckhout UGent, Geert Persiau UGent, Khadija Wahni, Nandita Bodra UGent, Inge Van Molle, Barbara De Smet UGent, Didier Vertommen, Kris Gevaert UGent, et al. (2014) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 111(31). p.11545-11550
abstract
Reactive oxygen species (ROS) have been shown to be potent signaling molecules. Today, oxidation of cysteine residues is a well-recognized posttranslational protein modification, but the signaling processes steered by such oxidations are poorly understood. To gain insight into the cysteine thiol-dependent ROS signaling in Arabidopsis thaliana, we identified the hydrogen peroxide (H2O2)-dependent sulfenome: that is, proteins with at least one cysteine thiol oxidized to a sulfenic acid. By means of a genetic construct consisting of a fusion between the C-terminal domain of the yeast (Saccharomyces cerevisiae) AP-1-like (YAP1) transcription factor and a tandem affinity purification tag, we detected similar to 100 sulfenylated proteins in Arabidopsis cell suspensions exposed to H2O2 stress. The in vivo YAP1-based trapping of sulfenylated proteins was validated by a targeted in vitro analysis of DEHYDROASCORBATE REDUCTASE2 (DHAR2). In DHAR2, the active site nucleophilic cysteine is regulated through a sulfenic acid-dependent switch, leading to S-glutathionylation, a protein modification that protects the protein against oxidative damage.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
TANDEM AFFINITY PURIFICATION, PROTEIN-TYROSINE PHOSPHATASES, REDOX REGULATION, EXPRESSION, ACID, IN-VIVO, DEHYDROASCORBATE REDUCTASE, PLANTS, KINASES, cysteine oxidation, oxidative stress, redox regulation, OXIDATIVE STRESS
journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Proc. Natl. Acad. Sci. USA
volume
111
issue
31
pages
11545 - 11550
Web of Science type
Article
Web of Science id
000339807200065
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
9.674 (2014)
JCR rank
4/57 (2014)
JCR quartile
1 (2014)
ISSN
0027-8424
DOI
10.1073/pnas.1411607111
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5709876
handle
http://hdl.handle.net/1854/LU-5709876
date created
2014-09-24 16:54:08
date last changed
2016-12-19 15:46:48
@article{5709876,
  abstract     = {Reactive oxygen species (ROS) have been shown to be potent signaling molecules. Today, oxidation of cysteine residues is a well-recognized posttranslational protein modification, but the signaling processes steered by such oxidations are poorly understood. To gain insight into the cysteine thiol-dependent ROS signaling in Arabidopsis thaliana, we identified the hydrogen peroxide (H2O2)-dependent sulfenome: that is, proteins with at least one cysteine thiol oxidized to a sulfenic acid. By means of a genetic construct consisting of a fusion between the C-terminal domain of the yeast (Saccharomyces cerevisiae) AP-1-like (YAP1) transcription factor and a tandem affinity purification tag, we detected similar to 100 sulfenylated proteins in Arabidopsis cell suspensions exposed to H2O2 stress. The in vivo YAP1-based trapping of sulfenylated proteins was validated by a targeted in vitro analysis of DEHYDROASCORBATE REDUCTASE2 (DHAR2). In DHAR2, the active site nucleophilic cysteine is regulated through a sulfenic acid-dependent switch, leading to S-glutathionylation, a protein modification that protects the protein against oxidative damage.},
  author       = {Waszczak, Cezary and Akter, Mosammat Salma and Eeckhout, Dominique and Persiau, Geert and Wahni, Khadija and Bodra, Nandita and Van Molle, Inge and De Smet, Barbara and Vertommen, Didier and Gevaert, Kris and De Jaeger, Geert and Van Montagu, Marc and Messens, Joris and Van Breusegem, Frank},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {TANDEM AFFINITY PURIFICATION,PROTEIN-TYROSINE PHOSPHATASES,REDOX REGULATION,EXPRESSION,ACID,IN-VIVO,DEHYDROASCORBATE REDUCTASE,PLANTS,KINASES,cysteine oxidation,oxidative stress,redox regulation,OXIDATIVE STRESS},
  language     = {eng},
  number       = {31},
  pages        = {11545--11550},
  title        = {Sulfenome mining in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1073/pnas.1411607111},
  volume       = {111},
  year         = {2014},
}

Chicago
Waszczak, Cezary, Mosammat Salma Akter, Dominique Eeckhout, Geert Persiau, Khadija Wahni, Nandita Bodra, Inge Van Molle, et al. 2014. “Sulfenome Mining in Arabidopsis Thaliana.” Proceedings of the National Academy of Sciences of the United States of America 111 (31): 11545–11550.
APA
Waszczak, C., Akter, M. S., Eeckhout, D., Persiau, G., Wahni, K., Bodra, N., Van Molle, I., et al. (2014). Sulfenome mining in Arabidopsis thaliana. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(31), 11545–11550.
Vancouver
1.
Waszczak C, Akter MS, Eeckhout D, Persiau G, Wahni K, Bodra N, et al. Sulfenome mining in Arabidopsis thaliana. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2014;111(31):11545–50.
MLA
Waszczak, Cezary, Mosammat Salma Akter, Dominique Eeckhout, et al. “Sulfenome Mining in Arabidopsis Thaliana.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 111.31 (2014): 11545–11550. Print.