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Mitochondrial perturbation negatively affects auxin signaling

Pavel Kerchev (UGent) , Inge De Clercq (UGent) , Jordi Denecker (UGent) , Per Mühlenbock (UGent) , Robert Kumpf (UGent) , Long Nguyen (UGent) , Dominique Audenaert (UGent) , Wim Dejonghe (UGent) and Frank Van Breusegem (UGent)
(2014) MOLECULAR PLANT. 7(7). p.1138-1150
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Mitochondria are crucial players in the signaling and metabolic homeostasis of the plant cell. The molecular components that orchestrate the underlying processes, however, are largely unknown. Using a chemical biology approach, we exploited the responsiveness of Arabidopsis UDP-glucosyltransferase-encoding UGT74E2 towards mitochondrial perturbation in order to look for novel mechanisms regulating mitochondria-to-nucleus communication. The most potent inducers of UGT74E2 shared a (2-furyl) acrylate (FAA) substructure that negatively affected mitochondrial function and was identified before as an auxin transcriptional inhibitor. Based on these premises, we demonstrated that perturbed mitochondria negatively affect the auxin signaling machinery. Moreover, chemical perturbation of polar auxin transport and auxin biosynthesis was sufficient to induce mitochondrial retrograde markers and their transcript abundance was constitutively elevated in the absence of the auxin transcriptional activators ARF7 and ARF19.
Keywords
ELECTRON-TRANSPORT, NAC TRANSCRIPTION FACTOR, ALTERNATIVE OXIDASE, OXIDATIVE STRESS, RETROGRADE REGULATION, LEAF MITOCHONDRIA, ARABIDOPSIS-THALIANA, PLANTS, OSMOTIC-STRESS, retrograde communication, mitochondrial perturbation, GENE-EXPRESSION, auxin signaling

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Chicago
Kerchev, Pavel, Inge De Clercq, Jordi Denecker, Per Mühlenbock, Robert Kumpf, Long Nguyen, Dominique Audenaert, Wim Dejonghe, and Frank Van Breusegem. 2014. “Mitochondrial Perturbation Negatively Affects Auxin Signaling.” Molecular Plant 7 (7): 1138–1150.
APA
Kerchev, P., De Clercq, I., Denecker, J., Mühlenbock, P., Kumpf, R., Nguyen, L., Audenaert, D., et al. (2014). Mitochondrial perturbation negatively affects auxin signaling. MOLECULAR PLANT, 7(7), 1138–1150.
Vancouver
1.
Kerchev P, De Clercq I, Denecker J, Mühlenbock P, Kumpf R, Nguyen L, et al. Mitochondrial perturbation negatively affects auxin signaling. MOLECULAR PLANT. 2014;7(7):1138–50.
MLA
Kerchev, Pavel, Inge De Clercq, Jordi Denecker, et al. “Mitochondrial Perturbation Negatively Affects Auxin Signaling.” MOLECULAR PLANT 7.7 (2014): 1138–1150. Print.
@article{5709527,
  abstract     = {Mitochondria are crucial players in the signaling and metabolic homeostasis of the plant cell. The molecular components that orchestrate the underlying processes, however, are largely unknown. Using a chemical biology approach, we exploited the responsiveness of Arabidopsis UDP-glucosyltransferase-encoding UGT74E2 towards mitochondrial perturbation in order to look for novel mechanisms regulating mitochondria-to-nucleus communication. The most potent inducers of UGT74E2 shared a (2-furyl) acrylate (FAA) substructure that negatively affected mitochondrial function and was identified before as an auxin transcriptional inhibitor. Based on these premises, we demonstrated that perturbed mitochondria negatively affect the auxin signaling machinery. Moreover, chemical perturbation of polar auxin transport and auxin biosynthesis was sufficient to induce mitochondrial retrograde markers and their transcript abundance was constitutively elevated in the absence of the auxin transcriptional activators ARF7 and ARF19.},
  author       = {Kerchev, Pavel and De Clercq, Inge and Denecker, Jordi and M{\"u}hlenbock, Per and Kumpf, Robert and Nguyen, Long and Audenaert, Dominique and Dejonghe, Wim and Van Breusegem, Frank},
  issn         = {1674-2052},
  journal      = {MOLECULAR PLANT},
  keyword      = {ELECTRON-TRANSPORT,NAC TRANSCRIPTION FACTOR,ALTERNATIVE OXIDASE,OXIDATIVE STRESS,RETROGRADE REGULATION,LEAF MITOCHONDRIA,ARABIDOPSIS-THALIANA,PLANTS,OSMOTIC-STRESS,retrograde communication,mitochondrial perturbation,GENE-EXPRESSION,auxin signaling},
  language     = {eng},
  number       = {7},
  pages        = {1138--1150},
  title        = {Mitochondrial perturbation negatively affects auxin signaling},
  url          = {http://dx.doi.org/10.1093/mp/ssu071},
  volume       = {7},
  year         = {2014},
}

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