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Gastric de novo Muc13 expression and spasmolytic polypeptide-expressing metaplasia during Helicobacter heilmannii infection

Cheng Liu (UGent) , Annemieke Smet (UGent) , Caroline Blaecher (UGent) , Bram Flahou (UGent) , Richard Ducatelle (UGent) , Sara Linden and Freddy Haesebrouck (UGent)
(2014) INFECTION AND IMMUNITY. 82(8). p.3227-3239
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Abstract
Helicobacter heilmannii is a zoonotic bacterium that has been associated with gastric disease in humans. In this study, the mRNA expression of mucins in the stomach of BALB/c mice was analyzed at several time points during a 1-year infection with this bacterium, during which gastric disease progressed in severity. Markers for acid production by parietal cells and mucous metaplasia were also examined. In the first 9 weeks postinfection, the mRNA expression of Muc6 was clearly upregulated in both the antrum and fundus of the stomach of H. heilmannii-infected mice. Interestingly, Muc13 was upregulated already at 1 day postinfection in the fundus of the stomach. Its expression level remained high in the stomach over the course of the infection. This mucin is, however, not expressed in a healthy stomach, and high expression of this mucin has so far only been described in gastric cancer. In the later stages of infection, mRNA expression of H+/K+-ATPase alpha/beta and KCNQ1 decreased, whereas the expression of Muc4, Tff2, Dmbt1, and polymeric immunoglobulin receptor (pIgR) increased starting at 16 weeks postinfection onwards, suggesting the existence of spasmolytic polypeptide-expressing metaplasia in the fundus of the stomach. Mucous metaplasia present in the mucosa surrounding low-grade mucosa-associated lymphoid tissue (MALT) lymphoma-like lesions was also histologically confirmed. Our findings indicate that H. heilmannii infection causes severe gastric pathologies and alterations in the expression pattern of gastric mucins, such as Muc6 and Muc13, as well as disrupting gastric homeostasis by inducing the loss of parietal cells, resulting in the development of mucous metaplasia.
Keywords
MUC5AC, CANCER, STOMACH, PROGRESSION, MUCOSA, PYLORI, SEQUENCE-ANALYSIS, INTESTINAL METAPLASIA, MALT-LYMPHOMA, MICE

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MLA
Liu, Cheng, Annemieke Smet, Caroline Blaecher, et al. “Gastric De Novo Muc13 Expression and Spasmolytic Polypeptide-expressing Metaplasia During Helicobacter Heilmannii Infection.” INFECTION AND IMMUNITY 82.8 (2014): 3227–3239. Print.
APA
Liu, Cheng, Smet, A., Blaecher, C., Flahou, B., Ducatelle, R., Linden, S., & Haesebrouck, F. (2014). Gastric de novo Muc13 expression and spasmolytic polypeptide-expressing metaplasia during Helicobacter heilmannii infection. INFECTION AND IMMUNITY, 82(8), 3227–3239.
Chicago author-date
Liu, Cheng, Annemieke Smet, Caroline Blaecher, Bram Flahou, Richard Ducatelle, Sara Linden, and Freddy Haesebrouck. 2014. “Gastric De Novo Muc13 Expression and Spasmolytic Polypeptide-expressing Metaplasia During Helicobacter Heilmannii Infection.” Infection and Immunity 82 (8): 3227–3239.
Chicago author-date (all authors)
Liu, Cheng, Annemieke Smet, Caroline Blaecher, Bram Flahou, Richard Ducatelle, Sara Linden, and Freddy Haesebrouck. 2014. “Gastric De Novo Muc13 Expression and Spasmolytic Polypeptide-expressing Metaplasia During Helicobacter Heilmannii Infection.” Infection and Immunity 82 (8): 3227–3239.
Vancouver
1.
Liu C, Smet A, Blaecher C, Flahou B, Ducatelle R, Linden S, et al. Gastric de novo Muc13 expression and spasmolytic polypeptide-expressing metaplasia during Helicobacter heilmannii infection. INFECTION AND IMMUNITY. 2014;82(8):3227–39.
IEEE
[1]
C. Liu et al., “Gastric de novo Muc13 expression and spasmolytic polypeptide-expressing metaplasia during Helicobacter heilmannii infection,” INFECTION AND IMMUNITY, vol. 82, no. 8, pp. 3227–3239, 2014.
@article{5709481,
  abstract     = {Helicobacter heilmannii is a zoonotic bacterium that has been associated with gastric disease in humans. In this study, the mRNA expression of mucins in the stomach of BALB/c mice was analyzed at several time points during a 1-year infection with this bacterium, during which gastric disease progressed in severity. Markers for acid production by parietal cells and mucous metaplasia were also examined. In the first 9 weeks postinfection, the mRNA expression of Muc6 was clearly upregulated in both the antrum and fundus of the stomach of H. heilmannii-infected mice. Interestingly, Muc13 was upregulated already at 1 day postinfection in the fundus of the stomach. Its expression level remained high in the stomach over the course of the infection. This mucin is, however, not expressed in a healthy stomach, and high expression of this mucin has so far only been described in gastric cancer. In the later stages of infection, mRNA expression of H+/K+-ATPase alpha/beta and KCNQ1 decreased, whereas the expression of Muc4, Tff2, Dmbt1, and polymeric immunoglobulin receptor (pIgR) increased starting at 16 weeks postinfection onwards, suggesting the existence of spasmolytic polypeptide-expressing metaplasia in the fundus of the stomach. Mucous metaplasia present in the mucosa surrounding low-grade mucosa-associated lymphoid tissue (MALT) lymphoma-like lesions was also histologically confirmed. Our findings indicate that H. heilmannii infection causes severe gastric pathologies and alterations in the expression pattern of gastric mucins, such as Muc6 and Muc13, as well as disrupting gastric homeostasis by inducing the loss of parietal cells, resulting in the development of mucous metaplasia.},
  author       = {Liu, Cheng and Smet, Annemieke and Blaecher, Caroline and Flahou, Bram and Ducatelle, Richard and Linden, Sara and Haesebrouck, Freddy},
  issn         = {0019-9567},
  journal      = {INFECTION AND IMMUNITY},
  keywords     = {MUC5AC,CANCER,STOMACH,PROGRESSION,MUCOSA,PYLORI,SEQUENCE-ANALYSIS,INTESTINAL METAPLASIA,MALT-LYMPHOMA,MICE},
  language     = {eng},
  number       = {8},
  pages        = {3227--3239},
  title        = {Gastric de novo Muc13 expression and spasmolytic polypeptide-expressing metaplasia during Helicobacter heilmannii infection},
  url          = {http://dx.doi.org/10.1128/IAI.01867-14},
  volume       = {82},
  year         = {2014},
}

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