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Development of new PCR primers by comparative genomics for the detection of Helicobacter suis in gastric biopsy specimens

Hidenori Matsui, Tetsufumi Takahashi, Somay Y Murayama, Ikuo Uchiyama, Katsushi Yamaguchi, Shuji Shigenobu, Takehisa Matsumoto, Masatomo Kawakubo, Kazuki Horiuchi, Hiroyoshi Ota, et al. (2014) HELICOBACTER. 19(4). p.260-271
abstract
Background: Although the infection rate of Helicobacter suis is significantly lower than that of Helicobacter pylori, the H. suis infection is associated with a high rate of gastric mucosa-associated lymphoid tissue (MALT) lymphoma. In addition, in vitro cultivation of H. suis remains difficult, and some H. suis-infected patients show negative results on the urea breath test (UBT). Materials and Methods: Female C57BL/6J mice were orally inoculated with mouse gastric mucosal homogenates containing H. suis strains TKY or SNTW101 isolated from a cynomolgus monkey or a patient suffering from nodular gastritis, respectively. The high-purity chromosomal DNA samples of H. suis strains TKY and SNTW101 were prepared from the infected mouse gastric mucosa. The SOLiD sequencing of two H. suis genomes enabled comparative genomics of 20 Helicobacter and 11 Campylobacter strains for the identification of the H. suis-specific nucleotide sequences. Results: Oral inoculation with mouse gastric mucosal homogenates containing H. suis strains TKY and SNTW101 induced gastric MALT lymphoma and the formation of gastric lymphoid follicles, respectively, in C57BL/6J mice. Two conserved nucleotide sequences among six H. suis strains were identified and were used to design diagnostic PCR primers for the detection of H. suis. Conclusions: There was a strong association between the H. suis infection and gastric diseases in the C57BL/6 mouse model. PCR diagnosis using an H. suis-specific primer pair is a valuable method for detecting H. suis in gastric biopsy specimens.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
INFECTION, MUCOSA, SP-NOV., C57BL/6 MICE, SENSU-STRICTO, PYLORI HELICOBACTERS, BIOFILM FORMATION, 16S RIBOSOMAL-RNA, GRADE MALT LYMPHOMA, HEILMANNII-LIKE ORGANISMS, PCR diagnosis, mouse infection model, gastric biopsy specimen, comparative genomics, next-generation sequencing, Helicobacter suis
journal title
HELICOBACTER
Helicobacter
volume
19
issue
4
pages
260 - 271
Web of Science type
Article
Web of Science id
000339953800003
JCR category
MICROBIOLOGY
JCR impact factor
4.106 (2014)
JCR rank
24/119 (2014)
JCR quartile
1 (2014)
ISSN
1083-4389
DOI
10.1111/hel.12127
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5709403
handle
http://hdl.handle.net/1854/LU-5709403
date created
2014-09-24 15:42:58
date last changed
2016-12-19 15:46:44
@article{5709403,
  abstract     = {Background: Although the infection rate of Helicobacter suis is significantly lower than that of Helicobacter pylori, the H. suis infection is associated with a high rate of gastric mucosa-associated lymphoid tissue (MALT) lymphoma. In addition, in vitro cultivation of H. suis remains difficult, and some H. suis-infected patients show negative results on the urea breath test (UBT). 
Materials and Methods: Female C57BL/6J mice were orally inoculated with mouse gastric mucosal homogenates containing H. suis strains TKY or SNTW101 isolated from a cynomolgus monkey or a patient suffering from nodular gastritis, respectively. The high-purity chromosomal DNA samples of H. suis strains TKY and SNTW101 were prepared from the infected mouse gastric mucosa. The SOLiD sequencing of two H. suis genomes enabled comparative genomics of 20 Helicobacter and 11 Campylobacter strains for the identification of the H. suis-specific nucleotide sequences. 
Results: Oral inoculation with mouse gastric mucosal homogenates containing H. suis strains TKY and SNTW101 induced gastric MALT lymphoma and the formation of gastric lymphoid follicles, respectively, in C57BL/6J mice. Two conserved nucleotide sequences among six H. suis strains were identified and were used to design diagnostic PCR primers for the detection of H. suis. 
Conclusions: There was a strong association between the H. suis infection and gastric diseases in the C57BL/6 mouse model. PCR diagnosis using an H. suis-specific primer pair is a valuable method for detecting H. suis in gastric biopsy specimens.},
  author       = {Matsui, Hidenori and Takahashi, Tetsufumi and Murayama, Somay Y and Uchiyama, Ikuo and Yamaguchi, Katsushi and Shigenobu, Shuji and Matsumoto, Takehisa and Kawakubo, Masatomo and Horiuchi, Kazuki and Ota, Hiroyoshi and Osaki, Takako and Kamiya, Shigeru and Smet, Annemieke and Flahou, Bram and Ducatelle, Richard and Haesebrouck, Freddy and Takahashi, Shinichi and Nakamura, Shinichi and Nakamura, Masahiko},
  issn         = {1083-4389},
  journal      = {HELICOBACTER},
  keyword      = {INFECTION,MUCOSA,SP-NOV.,C57BL/6 MICE,SENSU-STRICTO,PYLORI HELICOBACTERS,BIOFILM FORMATION,16S RIBOSOMAL-RNA,GRADE MALT LYMPHOMA,HEILMANNII-LIKE ORGANISMS,PCR diagnosis,mouse infection model,gastric biopsy specimen,comparative genomics,next-generation sequencing,Helicobacter suis},
  language     = {eng},
  number       = {4},
  pages        = {260--271},
  title        = {Development of new PCR primers by comparative genomics for the detection of Helicobacter suis in gastric biopsy specimens},
  url          = {http://dx.doi.org/10.1111/hel.12127},
  volume       = {19},
  year         = {2014},
}

Chicago
Matsui, Hidenori, Tetsufumi Takahashi, Somay Y Murayama, Ikuo Uchiyama, Katsushi Yamaguchi, Shuji Shigenobu, Takehisa Matsumoto, et al. 2014. “Development of New PCR Primers by Comparative Genomics for the Detection of Helicobacter Suis in Gastric Biopsy Specimens.” Helicobacter 19 (4): 260–271.
APA
Matsui, H., Takahashi, T., Murayama, S. Y., Uchiyama, I., Yamaguchi, K., Shigenobu, S., Matsumoto, T., et al. (2014). Development of new PCR primers by comparative genomics for the detection of Helicobacter suis in gastric biopsy specimens. HELICOBACTER, 19(4), 260–271.
Vancouver
1.
Matsui H, Takahashi T, Murayama SY, Uchiyama I, Yamaguchi K, Shigenobu S, et al. Development of new PCR primers by comparative genomics for the detection of Helicobacter suis in gastric biopsy specimens. HELICOBACTER. 2014;19(4):260–71.
MLA
Matsui, Hidenori, Tetsufumi Takahashi, Somay Y Murayama, et al. “Development of New PCR Primers by Comparative Genomics for the Detection of Helicobacter Suis in Gastric Biopsy Specimens.” HELICOBACTER 19.4 (2014): 260–271. Print.