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Analysis of the mucosal immune responses induced by single and trickle infections with the bovine abomasal nematode Ostertagia ostertagi

Belgacem Mihi, Frederik Van Meulder UGent, S Vancoppernolle, Manuela Rinaldi UGent, Koen Chiers UGent, Wim Van Den Broeck UGent, Bruno Goddeeris, Jozef Vercruysse UGent, Edwin Claerebout UGent and Peter Geldhof UGent (2014) PARASITE IMMUNOLOGY. 36(4). p.150-156
abstract
The purpose of this study was to provide more information on the kinetics of the immunological changes occurring in the abomasal mucosa after single and trickle infections with the bovine parasite Ostertagia ostertagi. The time course analysis of gene expression revealed that the major changes coincided with the emergence of adult worms from the gastric glands. These changes consisted of a simultaneous upregulation of Th1- and Th2-type cytokines. In addition, a single O.ostertagi infection elicited an upregulation of the epithelial-derived cytokine IL33, while TSLP expression levels were not impacted. Apart from the massive increase in inflammatory cytokines IL6, IL17 and IL21, O.ostertagi infection also elicited an upregulation of the immunosuppressors TGFB, IL10 and ARG1, as well as NK and -T cell markers. Furthermore, the cytotoxic factors granulysin, perforin and granzyme B were upregulated following an O.ostertagi infection. Analysis of cytokine transcript levels in animals receiving trickle infections for 60days showed a similar trend as observed following a single infection except for IL33, IL6, GATA-3, TBX21 and NCR1, which were no longer upregulated after trickle infections. Finally, the long trickle infections were associated with mucosal eosinophilia and mastocytosis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
parasite, ruminant, Ostertagia spp, immunopathology, host species, disease, GASTROINTESTINAL NEMATODES, GENE-EXPRESSION, CATTLE, CYTOKINES, CELLS, CALVES, QUANTIFICATION, LYMPHOCYTES, INTERLEUKIN-9, RESISTANCE
journal title
PARASITE IMMUNOLOGY
Parasite Immunol.
volume
36
issue
4
pages
150 - 156
Web of Science type
Article
Web of Science id
000332324700002
JCR category
PARASITOLOGY
JCR impact factor
2.143 (2014)
JCR rank
14/36 (2014)
JCR quartile
2 (2014)
ISSN
0141-9838
DOI
10.1111/pim.12094
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5705787
handle
http://hdl.handle.net/1854/LU-5705787
date created
2014-09-19 10:20:54
date last changed
2016-12-19 15:42:18
@article{5705787,
  abstract     = {The purpose of this study was to provide more information on the kinetics of the immunological changes occurring in the abomasal mucosa after single and trickle infections with the bovine parasite Ostertagia ostertagi. The time course analysis of gene expression revealed that the major changes coincided with the emergence of adult worms from the gastric glands. These changes consisted of a simultaneous upregulation of Th1- and Th2-type cytokines. In addition, a single O.ostertagi infection elicited an upregulation of the epithelial-derived cytokine IL33, while TSLP expression levels were not impacted. Apart from the massive increase in inflammatory cytokines IL6, IL17 and IL21, O.ostertagi infection also elicited an upregulation of the immunosuppressors TGFB, IL10 and ARG1, as well as NK and -T cell markers. Furthermore, the cytotoxic factors granulysin, perforin and granzyme B were upregulated following an O.ostertagi infection. Analysis of cytokine transcript levels in animals receiving trickle infections for 60days showed a similar trend as observed following a single infection except for IL33, IL6, GATA-3, TBX21 and NCR1, which were no longer upregulated after trickle infections. Finally, the long trickle infections were associated with mucosal eosinophilia and mastocytosis.},
  author       = {Mihi, Belgacem and Van Meulder, Frederik and Vancoppernolle, S and Rinaldi, Manuela and Chiers, Koen and Van Den Broeck, Wim and Goddeeris, Bruno and Vercruysse, Jozef and Claerebout, Edwin and Geldhof, Peter},
  issn         = {0141-9838},
  journal      = {PARASITE IMMUNOLOGY},
  keyword      = {parasite,ruminant,Ostertagia spp,immunopathology,host species,disease,GASTROINTESTINAL NEMATODES,GENE-EXPRESSION,CATTLE,CYTOKINES,CELLS,CALVES,QUANTIFICATION,LYMPHOCYTES,INTERLEUKIN-9,RESISTANCE},
  language     = {eng},
  number       = {4},
  pages        = {150--156},
  title        = {Analysis of the mucosal immune responses induced by single and trickle infections with the bovine abomasal nematode Ostertagia ostertagi},
  url          = {http://dx.doi.org/10.1111/pim.12094},
  volume       = {36},
  year         = {2014},
}

Chicago
Mihi, Belgacem, Frederik Van Meulder, S Vancoppernolle, Manuela Rinaldi, Koen Chiers, Wim Van Den Broeck, Bruno Goddeeris, Jozef Vercruysse, Edwin Claerebout, and Peter Geldhof. 2014. “Analysis of the Mucosal Immune Responses Induced by Single and Trickle Infections with the Bovine Abomasal Nematode Ostertagia Ostertagi.” Parasite Immunology 36 (4): 150–156.
APA
Mihi, B., Van Meulder, F., Vancoppernolle, S., Rinaldi, M., Chiers, K., Van Den Broeck, W., Goddeeris, B., et al. (2014). Analysis of the mucosal immune responses induced by single and trickle infections with the bovine abomasal nematode Ostertagia ostertagi. PARASITE IMMUNOLOGY, 36(4), 150–156.
Vancouver
1.
Mihi B, Van Meulder F, Vancoppernolle S, Rinaldi M, Chiers K, Van Den Broeck W, et al. Analysis of the mucosal immune responses induced by single and trickle infections with the bovine abomasal nematode Ostertagia ostertagi. PARASITE IMMUNOLOGY. 2014;36(4):150–6.
MLA
Mihi, Belgacem, Frederik Van Meulder, S Vancoppernolle, et al. “Analysis of the Mucosal Immune Responses Induced by Single and Trickle Infections with the Bovine Abomasal Nematode Ostertagia Ostertagi.” PARASITE IMMUNOLOGY 36.4 (2014): 150–156. Print.