Ghent University Academic Bibliography

Advanced

Fast nuclear staining of head hair roots as a screening method for successful STR analysis in forensics

Trees Lepez UGent, Mado Vandewoestyne UGent, David Van Hoofstat UGent and Dieter Deforce UGent (2014) FORENSIC SCIENCE INTERNATIONAL-GENETICS. 13. p.191-194
abstract
The success rate of STR profiling of hairs found at a crime scene is quite low and negative results of hair analysis are frequently reported. To increase the success rate of DNA analysis of hairs in forensics, nuclei in hair roots can be counted after staining the hair root with DAPI. Two staining methods were tested: a longer method with two 1 h incubations in respectively a DAPI-and a wash-solution, and a fast, direct staining of the hair root on microscope slides. The two staining methods were not significantly different. The results of the STR analysis for both procedures showed that 20 nuclei are necessary to obtain at least partial STR profiles. When more than 50 nuclei were counted, full STR profiles were always obtained. In 96% of the cases where no nuclei were detected, no STR profile could be obtained. However, 4% of the DAPI-negative hair roots resulted in at least partial STR profiles. Therefore, each forensic case has to be evaluated separately in function of the importance of the evidential value of the found hair. The fast staining method was applied in 36 forensic cases on 279 hairs in total. A fast screening method using DAPI can be used to increase the success rate of hair analysis in forensics.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Hair roots, DAPI, Fluorescence microscopy, Short Tandem Repeat (STR) analysis, Forensics, DNA, EXTRACTION
journal title
FORENSIC SCIENCE INTERNATIONAL-GENETICS
Forensic Sci. Int.-Genet.
volume
13
pages
191 - 194
Web of Science type
Article
Web of Science id
000342444400036
JCR category
MEDICINE, LEGAL
JCR impact factor
4.604 (2014)
JCR rank
1/15 (2014)
JCR quartile
1 (2014)
ISSN
1872-4973
DOI
10.1016/j.fsigen.2014.08.003
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
5704406
handle
http://hdl.handle.net/1854/LU-5704406
date created
2014-09-17 15:57:54
date last changed
2017-06-09 08:47:07
@article{5704406,
  abstract     = {The success rate of STR profiling of hairs found at a crime scene is quite low and negative results of hair analysis are frequently reported. To increase the success rate of DNA analysis of hairs in forensics, nuclei in hair roots can be counted after staining the hair root with DAPI. Two staining methods were tested: a longer method with two 1 h incubations in respectively a DAPI-and a wash-solution, and a fast, direct staining of the hair root on microscope slides. 
The two staining methods were not significantly different. The results of the STR analysis for both procedures showed that 20 nuclei are necessary to obtain at least partial STR profiles. When more than 50 nuclei were counted, full STR profiles were always obtained. In 96\% of the cases where no nuclei were detected, no STR profile could be obtained. However, 4\% of the DAPI-negative hair roots resulted in at least partial STR profiles. Therefore, each forensic case has to be evaluated separately in function of the importance of the evidential value of the found hair. The fast staining method was applied in 36 forensic cases on 279 hairs in total. 
A fast screening method using DAPI can be used to increase the success rate of hair analysis in forensics.},
  author       = {Lepez, Trees and Vandewoestyne, Mado and Van Hoofstat, David and Deforce, Dieter},
  issn         = {1872-4973},
  journal      = {FORENSIC SCIENCE INTERNATIONAL-GENETICS},
  keyword      = {Hair roots,DAPI,Fluorescence microscopy,Short Tandem Repeat (STR) analysis,Forensics,DNA,EXTRACTION},
  language     = {eng},
  pages        = {191--194},
  title        = {Fast nuclear staining of head hair roots as a screening method for successful STR analysis in forensics},
  url          = {http://dx.doi.org/10.1016/j.fsigen.2014.08.003},
  volume       = {13},
  year         = {2014},
}

Chicago
Lepez, Trees, Mado Vandewoestyne, David Van Hoofstat, and Dieter Deforce. 2014. “Fast Nuclear Staining of Head Hair Roots as a Screening Method for Successful STR Analysis in Forensics.” Forensic Science International-genetics 13: 191–194.
APA
Lepez, T., Vandewoestyne, M., Van Hoofstat, D., & Deforce, D. (2014). Fast nuclear staining of head hair roots as a screening method for successful STR analysis in forensics. FORENSIC SCIENCE INTERNATIONAL-GENETICS, 13, 191–194.
Vancouver
1.
Lepez T, Vandewoestyne M, Van Hoofstat D, Deforce D. Fast nuclear staining of head hair roots as a screening method for successful STR analysis in forensics. FORENSIC SCIENCE INTERNATIONAL-GENETICS. 2014;13:191–4.
MLA
Lepez, Trees, Mado Vandewoestyne, David Van Hoofstat, et al. “Fast Nuclear Staining of Head Hair Roots as a Screening Method for Successful STR Analysis in Forensics.” FORENSIC SCIENCE INTERNATIONAL-GENETICS 13 (2014): 191–194. Print.