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Histochemical and immunohistochemical evaluation of nerve regeneration using fibrin-agarose and adipose derived mesenchymal stem cells

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Abstract
The use of filled conduits improves the peripheral nerve regeneration. In this sense, the aim of this work is to determinate the usefulness of fibrin-agarose (FA) hydrogels and adipose derived mesenchymal stem cells (ADMSCs) in a rat model (10-mm). Male Wistar rats were used (n=36), and the gap of each animal was repaired using a NeuraGen® collagen conduit; collagen conduit/FA and collagen filled with FA/ADMSCs. After 12 weeks, histological analysis was performed. The intensity and area-fraction for the positive reaction of the collagen, myelin and the markers S100, laminin and neurofilament, were determined. Our results demonstrate that the use of FA/ADMSCs was significantly more efficient at the histological level.

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Chicago
Carriel Araya, Víctor Sebastián, Olga Roda, Ingrid Garzón, Maria Cornelissen, and Miguel Alaminos. 2014. “Histochemical and Immunohistochemical Evaluation of Nerve Regeneration Using Fibrin-agarose and Adipose Derived Mesenchymal Stem Cells.” In 2nd International Symposium on Peripheral Nerve Regeneration, in Join with the 3rd “Club Brunelli” Meeting, 9–9.
APA
Carriel Araya, V. S., Roda, O., Garzón, I., Cornelissen, M., & Alaminos, M. (2014). Histochemical and immunohistochemical evaluation of nerve regeneration using fibrin-agarose and adipose derived mesenchymal stem cells. 2nd International symposium on Peripheral Nerve Regeneration, in join with the 3rd “Club Brunelli” meeting (pp. 9–9). Presented at the 2nd International symposium on Peripheral Nerve Regeneration.
Vancouver
1.
Carriel Araya VS, Roda O, Garzón I, Cornelissen M, Alaminos M. Histochemical and immunohistochemical evaluation of nerve regeneration using fibrin-agarose and adipose derived mesenchymal stem cells. 2nd International symposium on Peripheral Nerve Regeneration, in join with the 3rd “Club Brunelli” meeting. 2014. p. 9–9.
MLA
Carriel Araya, Víctor Sebastián, Olga Roda, Ingrid Garzón, et al. “Histochemical and Immunohistochemical Evaluation of Nerve Regeneration Using Fibrin-agarose and Adipose Derived Mesenchymal Stem Cells.” 2nd International Symposium on Peripheral Nerve Regeneration, in Join with the 3rd “Club Brunelli” Meeting. 2014. 9–9. Print.
@inproceedings{5695143,
  abstract     = {The use of filled conduits improves the peripheral nerve regeneration. In this sense, the aim of this work is to determinate the usefulness of fibrin-agarose (FA) hydrogels and adipose derived mesenchymal stem cells (ADMSCs) in a rat model (10-mm). Male Wistar rats were used (n=36), and the gap of each animal was repaired using a NeuraGen{\textregistered} collagen conduit; collagen conduit/FA and collagen filled with FA/ADMSCs. After 12 weeks, histological analysis was performed. The intensity and area-fraction for the positive reaction of the collagen, myelin and the markers S100, laminin and neurofilament, were determined. Our results demonstrate that the use of FA/ADMSCs was significantly more efficient at the histological level.},
  author       = {Carriel Araya, V{\'i}ctor Sebasti{\'a}n and Roda, Olga and Garz{\'o}n, Ingrid and Cornelissen, Maria and Alaminos, Miguel},
  booktitle    = {2nd International symposium on Peripheral Nerve Regeneration, in join with the 3rd 'Club Brunelli' meeting},
  language     = {eng},
  location     = {Torino, Italy},
  pages        = {9--9},
  title        = {Histochemical and immunohistochemical evaluation of nerve regeneration using fibrin-agarose and adipose derived mesenchymal stem cells},
  year         = {2014},
}