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Development of an oral mucosa model to study host-microbiome interactions during wound healing

Tine De Ryck, Charlotte Grootaert UGent, Laura Jaspaert, Frederiek-Maarten Kerckhof UGent, Mireille Van Gele UGent, Joachim De Schrijver UGent, Pieter Van den Abbeele, Simon Swift, Marc Bracke UGent, Tom Van de Wiele UGent, et al. (2014) APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 98(15). p.6831-6846
abstract
Crosstalk between the human host and its microbiota is reported to influence various diseases such as mucositis. Fundamental research in this area is however complicated by the time frame restrictions during which host-microbe interactions can be studied in vitro. The model proposed in this paper, consisting of an oral epithelium and biofilm, can be used to study microbe-host crosstalk in vitro in non-infectious conditions up to 72 h. Microbiota derived from oral swabs were cultured on an agar/mucin layer and challenged with monolayers of keratinocytes grown on plastic or collagen type I layers embedded with fibroblasts. The overall microbial biofilm composition in terms of diversity remained representative for the oral microbiome, whilst the epithelial cell morphology and viability were unaffected. Applying the model to investigate wound healing revealed a reduced healing of 30 % in the presence of microbiota, which was not caused by a reduction of the proliferation index (52.1-61.5) or a significantly increased number of apoptotic (1-1.13) or necrotic (32-30.5 %) cells. Since the model allows the separate study of the microbial and cellular exometabolome, the biofilm and epithelial characteristics after co-culturing, it is applicable for investigations within fundamental research and for the discovery and development of agents that promote wound healing.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Biofilm, Epithelium, Co-culture, In vitro model, Oral mucosa, Wound healing, HUMAN GINGIVAL FIBROBLASTS, CHAIN FATTY-ACIDS, IN-VITRO, ANTIMICROBIAL PEPTIDES, STAPHYLOCOCCUS-AUREUS, BACTERIA, INFECTION, BIOFILMS, CAVITY, CELLS
journal title
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Appl. Microbiol. Biotechnol.
volume
98
issue
15
pages
6831 - 6846
Web of Science type
Article
Web of Science id
000339880300027
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.337 (2014)
JCR rank
39/163 (2014)
JCR quartile
1 (2014)
ISSN
0175-7598
DOI
10.1007/s00253-014-5841-1
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5675948
handle
http://hdl.handle.net/1854/LU-5675948
date created
2014-08-22 11:03:00
date last changed
2016-12-19 15:39:16
@article{5675948,
  abstract     = {Crosstalk between the human host and its microbiota is reported to influence various diseases such as mucositis. Fundamental research in this area is however complicated by the time frame restrictions during which host-microbe interactions can be studied in vitro. The model proposed in this paper, consisting of an oral epithelium and biofilm, can be used to study microbe-host crosstalk in vitro in non-infectious conditions up to 72 h. Microbiota derived from oral swabs were cultured on an agar/mucin layer and challenged with monolayers of keratinocytes grown on plastic or collagen type I layers embedded with fibroblasts. The overall microbial biofilm composition in terms of diversity remained representative for the oral microbiome, whilst the epithelial cell morphology and viability were unaffected. Applying the model to investigate wound healing revealed a reduced healing of 30 \% in the presence of microbiota, which was not caused by a reduction of the proliferation index (52.1-61.5) or a significantly increased number of apoptotic (1-1.13) or necrotic (32-30.5 \%) cells. Since the model allows the separate study of the microbial and cellular exometabolome, the biofilm and epithelial characteristics after co-culturing, it is applicable for investigations within fundamental research and for the discovery and development of agents that promote wound healing.},
  author       = {De Ryck, Tine and Grootaert, Charlotte and Jaspaert, Laura and Kerckhof, Frederiek-Maarten and Van Gele, Mireille and De Schrijver, Joachim and Van den Abbeele, Pieter and Swift, Simon and Bracke, Marc and Van de Wiele, Tom and Vanhoecke, Barbara},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keyword      = {Biofilm,Epithelium,Co-culture,In vitro model,Oral mucosa,Wound healing,HUMAN GINGIVAL FIBROBLASTS,CHAIN FATTY-ACIDS,IN-VITRO,ANTIMICROBIAL PEPTIDES,STAPHYLOCOCCUS-AUREUS,BACTERIA,INFECTION,BIOFILMS,CAVITY,CELLS},
  language     = {eng},
  number       = {15},
  pages        = {6831--6846},
  title        = {Development of an oral mucosa model to study host-microbiome interactions during wound healing},
  url          = {http://dx.doi.org/10.1007/s00253-014-5841-1},
  volume       = {98},
  year         = {2014},
}

Chicago
De Ryck, Tine, Charlotte Grootaert, Laura Jaspaert, Frederiek-Maarten Kerckhof, Mireille Van Gele, Joachim De Schrijver, Pieter Van den Abbeele, et al. 2014. “Development of an Oral Mucosa Model to Study Host-microbiome Interactions During Wound Healing.” Applied Microbiology and Biotechnology 98 (15): 6831–6846.
APA
De Ryck, T., Grootaert, C., Jaspaert, L., Kerckhof, F.-M., Van Gele, M., De Schrijver, J., Van den Abbeele, P., et al. (2014). Development of an oral mucosa model to study host-microbiome interactions during wound healing. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(15), 6831–6846.
Vancouver
1.
De Ryck T, Grootaert C, Jaspaert L, Kerckhof F-M, Van Gele M, De Schrijver J, et al. Development of an oral mucosa model to study host-microbiome interactions during wound healing. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2014;98(15):6831–46.
MLA
De Ryck, Tine, Charlotte Grootaert, Laura Jaspaert, et al. “Development of an Oral Mucosa Model to Study Host-microbiome Interactions During Wound Healing.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.15 (2014): 6831–6846. Print.