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Shear stress and VEGF enhance endothelial differentiation of human adipose-derived stem cells

(2014) GROWTH FACTORS. 32(5). p.139-149
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Abstract
Herein we combine chemical and mechanical stimulation to investigate the effects of vascular endothelial growth factor (VEGF) and physiological shear stress in promoting the differentiation human adipose derived stem cells (ADSCs) into endothelial cells. ADSCs were isolated and characterized; endothelial differentiation was promoted by culturing confluent cells in 50ng/ml VEGF under physiological shear stress for up to 14 days. Afterwards, endothelial cells were seeded onto collagen or acellular aortic valve matrices and exposed to 4 culture conditions: shear stress+VEGF; shear stress-VEGF; static+VEGF and static-VEGF. After 7 days, phenotype was investigated. ADSCs subjected to shear stress and VEGF express a comprehensive range of specific endothelial markers(vWF, eNOS and FLT-1 after 7 days and CD31, FLk-1 and VE-cadherinafter 14 days)and maintainthe phenotype when seeded onto scaffolds. Our protocol proved to bean efficient source of endothelial-like cells for tissue engineering based on autologous adipose-derived stem cell.
Keywords
shear stress, PROCESSED LIPOASPIRATE CELLS, vascular endothelial growth factor, collagen scaffold, differentiation, adipose-derived stem cells, STROMAL CELLS, IN-VITRO, HEART, INDUCTION, MURINE, VIVO

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Chicago
Colazzo, Francesca, Fahad Alrashed, Padmini Saratchandra, Ivan Carubelli, Adrian H Chester, Magdi H Yacoub, Patricia M Taylor, and Pamela Somers. 2014. “Shear Stress and VEGF Enhance Endothelial Differentiation of Human Adipose-derived Stem Cells.” Growth Factors 32 (5): 139–149.
APA
Colazzo, F., Alrashed, F., Saratchandra, P., Carubelli, I., Chester, A. H., Yacoub, M. H., Taylor, P. M., et al. (2014). Shear stress and VEGF enhance endothelial differentiation of human adipose-derived stem cells. GROWTH FACTORS, 32(5), 139–149.
Vancouver
1.
Colazzo F, Alrashed F, Saratchandra P, Carubelli I, Chester AH, Yacoub MH, et al. Shear stress and VEGF enhance endothelial differentiation of human adipose-derived stem cells. GROWTH FACTORS. 2014;32(5):139–49.
MLA
Colazzo, Francesca, Fahad Alrashed, Padmini Saratchandra, et al. “Shear Stress and VEGF Enhance Endothelial Differentiation of Human Adipose-derived Stem Cells.” GROWTH FACTORS 32.5 (2014): 139–149. Print.
@article{5673735,
  abstract     = {Herein we combine chemical and mechanical stimulation to investigate the effects of vascular endothelial growth factor (VEGF) and physiological shear stress in promoting the differentiation human adipose derived stem cells (ADSCs) into endothelial cells. 
ADSCs were isolated and characterized; endothelial differentiation was promoted by culturing confluent cells in 50ng/ml VEGF under physiological shear stress for up to 14 days. Afterwards, endothelial cells were seeded onto collagen or acellular aortic valve matrices and exposed to 4 culture conditions: shear stress+VEGF; shear stress-VEGF; static+VEGF and static-VEGF. After 7 days, phenotype was investigated.
ADSCs subjected to shear stress and VEGF express a comprehensive range of specific endothelial markers(vWF, eNOS and FLT-1 after 7 days and CD31, FLk-1 and VE-cadherinafter 14 days)and maintainthe phenotype when seeded onto scaffolds. Our protocol proved to bean efficient source of endothelial-like cells for tissue engineering based on autologous adipose-derived stem cell.},
  author       = {Colazzo, Francesca and Alrashed, Fahad and Saratchandra, Padmini and Carubelli, Ivan and Chester, Adrian H and Yacoub, Magdi H and Taylor, Patricia M and Somers, Pamela},
  issn         = {0897-7194},
  journal      = {GROWTH FACTORS},
  keyword      = {shear stress,PROCESSED LIPOASPIRATE CELLS,vascular endothelial growth factor,collagen scaffold,differentiation,adipose-derived stem cells,STROMAL CELLS,IN-VITRO,HEART,INDUCTION,MURINE,VIVO},
  language     = {eng},
  number       = {5},
  pages        = {139--149},
  title        = {Shear stress and VEGF enhance endothelial differentiation of human adipose-derived stem cells},
  url          = {http://dx.doi.org/10.3109/08977194.2014.945642},
  volume       = {32},
  year         = {2014},
}

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