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Shear stress and VEGF enhance endothelial differentiation of human adipose-derived stem cells

Francesca Colazzo, Fahad Alrashed, Padmini Saratchandra, Ivan Carubelli, Adrian H Chester, Magdi H Yacoub, Patricia M Taylor and Pamela Somers (2014) GROWTH FACTORS. 32(5). p.139-149
abstract
Herein we combine chemical and mechanical stimulation to investigate the effects of vascular endothelial growth factor (VEGF) and physiological shear stress in promoting the differentiation human adipose derived stem cells (ADSCs) into endothelial cells. ADSCs were isolated and characterized; endothelial differentiation was promoted by culturing confluent cells in 50ng/ml VEGF under physiological shear stress for up to 14 days. Afterwards, endothelial cells were seeded onto collagen or acellular aortic valve matrices and exposed to 4 culture conditions: shear stress+VEGF; shear stress-VEGF; static+VEGF and static-VEGF. After 7 days, phenotype was investigated. ADSCs subjected to shear stress and VEGF express a comprehensive range of specific endothelial markers(vWF, eNOS and FLT-1 after 7 days and CD31, FLk-1 and VE-cadherinafter 14 days)and maintainthe phenotype when seeded onto scaffolds. Our protocol proved to bean efficient source of endothelial-like cells for tissue engineering based on autologous adipose-derived stem cell.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
shear stress, PROCESSED LIPOASPIRATE CELLS, vascular endothelial growth factor, collagen scaffold, differentiation, adipose-derived stem cells, STROMAL CELLS, IN-VITRO, HEART, INDUCTION, MURINE, VIVO
journal title
GROWTH FACTORS
Growth Factors
volume
32
issue
5
pages
139 - 149
Web of Science type
Article
Web of Science id
000343001700001
JCR category
ENDOCRINOLOGY & METABOLISM
JCR impact factor
3.386 (2014)
JCR rank
50/128 (2014)
JCR quartile
2 (2014)
ISSN
0897-7194
DOI
10.3109/08977194.2014.945642
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5673735
handle
http://hdl.handle.net/1854/LU-5673735
date created
2014-08-18 09:18:17
date last changed
2016-12-19 15:47:30
@article{5673735,
  abstract     = {Herein we combine chemical and mechanical stimulation to investigate the effects of vascular endothelial growth factor (VEGF) and physiological shear stress in promoting the differentiation human adipose derived stem cells (ADSCs) into endothelial cells. 
ADSCs were isolated and characterized; endothelial differentiation was promoted by culturing confluent cells in 50ng/ml VEGF under physiological shear stress for up to 14 days. Afterwards, endothelial cells were seeded onto collagen or acellular aortic valve matrices and exposed to 4 culture conditions: shear stress+VEGF; shear stress-VEGF; static+VEGF and static-VEGF. After 7 days, phenotype was investigated.
ADSCs subjected to shear stress and VEGF express a comprehensive range of specific endothelial markers(vWF, eNOS and FLT-1 after 7 days and CD31, FLk-1 and VE-cadherinafter 14 days)and maintainthe phenotype when seeded onto scaffolds. Our protocol proved to bean efficient source of endothelial-like cells for tissue engineering based on autologous adipose-derived stem cell.},
  author       = {Colazzo, Francesca and Alrashed, Fahad and Saratchandra, Padmini and Carubelli, Ivan and Chester, Adrian H and Yacoub, Magdi H and Taylor, Patricia M and Somers, Pamela},
  issn         = {0897-7194},
  journal      = {GROWTH FACTORS},
  keyword      = {shear stress,PROCESSED LIPOASPIRATE CELLS,vascular endothelial growth factor,collagen scaffold,differentiation,adipose-derived stem cells,STROMAL CELLS,IN-VITRO,HEART,INDUCTION,MURINE,VIVO},
  language     = {eng},
  number       = {5},
  pages        = {139--149},
  title        = {Shear stress and VEGF enhance endothelial differentiation of human adipose-derived stem cells},
  url          = {http://dx.doi.org/10.3109/08977194.2014.945642},
  volume       = {32},
  year         = {2014},
}

Chicago
Colazzo, Francesca, Fahad Alrashed, Padmini Saratchandra, Ivan Carubelli, Adrian H Chester, Magdi H Yacoub, Patricia M Taylor, and Pamela Somers. 2014. “Shear Stress and VEGF Enhance Endothelial Differentiation of Human Adipose-derived Stem Cells.” Growth Factors 32 (5): 139–149.
APA
Colazzo, F., Alrashed, F., Saratchandra, P., Carubelli, I., Chester, A. H., Yacoub, M. H., Taylor, P. M., et al. (2014). Shear stress and VEGF enhance endothelial differentiation of human adipose-derived stem cells. GROWTH FACTORS, 32(5), 139–149.
Vancouver
1.
Colazzo F, Alrashed F, Saratchandra P, Carubelli I, Chester AH, Yacoub MH, et al. Shear stress and VEGF enhance endothelial differentiation of human adipose-derived stem cells. GROWTH FACTORS. 2014;32(5):139–49.
MLA
Colazzo, Francesca, Fahad Alrashed, Padmini Saratchandra, et al. “Shear Stress and VEGF Enhance Endothelial Differentiation of Human Adipose-derived Stem Cells.” GROWTH FACTORS 32.5 (2014): 139–149. Print.