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Characterization of 27 mycotoxin detoxifiers and the relation with in vitro zearalenone binding

Thomas De Mil (UGent) , Mathias Devreese (UGent) , Nathan Broekaert (UGent) , Sophie Fraeyman (UGent) , Eric Van Ranst (UGent) , Sarah De Saeger (UGent) , Mia Eeckhout (UGent) , Geert Haesaert (UGent) , Patrick De Backer (UGent) and Siska Croubels (UGent)
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Abstract
Addition of mycotoxin detoxifiers to feed is a common practice to counteract the deleterious effects of mycotoxins on animal health. Although a variety of this kind of feed additives is available on the market, little is known about their detailed composition and physico-chemical properties. This lack of knowledge might pose some difficulties for researchers and users to compare and evaluate the efficacy and safety of these additives. The present study describes the characterization of 27 commercially available mycotoxin detoxifiers collected from various sources in Flanders and The Netherlands. Characterization comprised XRD-profiling of the mineral content, determination of the cation exchange capacity and exchangeable cations, acidity, mineral fraction, humidity and swelling volume. The XRD- and mineral fraction data were analysed with principal component analysis and explorative cluster analysis which enabled the identification of three distinct groups: smectite based (n=19), non-smectite based (n=5) and organic based detoxifiers (n=4). In a second experiment, an in vitro zearalenone binding test was conducted using phosphate buffered saline at pH 2.5, 6.5 and 8.0. The zearalenone binding of the smectite based group was related to the physico-chemical properties using a multivariate linear regression model. The binding in the non-smectite based and organic based groups was analysed using a one-way ANOVA. A large variability in binding properties was present, especially in the smectite containing group. Additives with smectite-containing mixed-layered minerals and additives containing humic acids expressed the highest binding which amounted to over 90%. The retained multivariate linear model included exchangeable potassium (p≤0.05), moist content (p≤0.05) and mineral fraction (0.05≤p≤0.1), indicating a statistical relation of these parameters with the in vitro zearalenone binding.

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Chicago
De Mil, Thomas, Mathias Devreese, Nathan Broekaert, Sophie Fraeyman, Eric Van Ranst, Sarah De Saeger, Mia Eeckhout, Geert Haesaert, Patrick De Backer, and Siska Croubels. 2014. “Characterization of 27 Mycotoxin Detoxifiers and the Relation with in Vitro Zearalenone Binding.” In 36th Mycotoxin Workshop : Conference Abstracts. Gesellschaft für Mykotoxinforschung.
APA
De Mil, Thomas, Devreese, M., Broekaert, N., Fraeyman, S., Van Ranst, E., De Saeger, S., Eeckhout, M., et al. (2014). Characterization of 27 mycotoxin detoxifiers and the relation with in vitro zearalenone binding. 36th Mycotoxin workshop : conference abstracts. Presented at the 36th Mycotoxin workshop, Gesellschaft für Mykotoxinforschung.
Vancouver
1.
De Mil T, Devreese M, Broekaert N, Fraeyman S, Van Ranst E, De Saeger S, et al. Characterization of 27 mycotoxin detoxifiers and the relation with in vitro zearalenone binding. 36th Mycotoxin workshop : conference abstracts. Gesellschaft für Mykotoxinforschung; 2014.
MLA
De Mil, Thomas, Mathias Devreese, Nathan Broekaert, et al. “Characterization of 27 Mycotoxin Detoxifiers and the Relation with in Vitro Zearalenone Binding.” 36th Mycotoxin Workshop : Conference Abstracts. Gesellschaft für Mykotoxinforschung, 2014. Print.
@inproceedings{5671796,
  abstract     = {Addition of mycotoxin detoxifiers to feed is a common practice to counteract the deleterious effects of mycotoxins on animal health. Although a variety of this kind of feed additives is available on the market, little is known about their detailed composition and physico-chemical properties. This lack of knowledge might pose some difficulties for researchers and users to compare and evaluate the efficacy and safety of these additives.
The present study describes the characterization of 27 commercially available mycotoxin detoxifiers collected from various sources in Flanders and The Netherlands. Characterization comprised XRD-profiling of the mineral content, determination of the cation exchange capacity and exchangeable cations, acidity, mineral fraction, humidity and swelling volume. The XRD- and mineral fraction data were analysed with principal component analysis and explorative cluster analysis which enabled the identification of three distinct groups: smectite based (n=19), non-smectite based (n=5) and organic based detoxifiers (n=4). In a second experiment, an in vitro zearalenone binding test was conducted using phosphate buffered saline at pH 2.5, 6.5 and 8.0. The zearalenone binding of the smectite based group was related to the physico-chemical properties using a multivariate linear regression model. The binding in the non-smectite based and organic based groups was analysed using a one-way ANOVA.
A large variability in binding properties was present, especially in the smectite containing group. Additives with smectite-containing mixed-layered minerals and additives containing humic acids expressed the highest binding which amounted to over 90\%. The retained multivariate linear model included exchangeable potassium (p\ensuremath{\leq}0.05), moist content (p\ensuremath{\leq}0.05) and mineral fraction (0.05\ensuremath{\leq}p\ensuremath{\leq}0.1), indicating a statistical relation of these parameters with the in vitro zearalenone binding.},
  author       = {De Mil, Thomas and Devreese, Mathias and Broekaert, Nathan and Fraeyman, Sophie and Van Ranst, Eric and De Saeger, Sarah and Eeckhout, Mia and Haesaert, Geert and De Backer, Patrick and Croubels, Siska},
  booktitle    = {36th Mycotoxin workshop : conference abstracts},
  language     = {eng},
  location     = {G{\"o}ttingen, Germany},
  publisher    = {Gesellschaft f{\"u}r Mykotoxinforschung},
  title        = {Characterization of 27 mycotoxin detoxifiers and the relation with in vitro zearalenone binding},
  year         = {2014},
}