Advanced search
1 file | 2.24 MB Add to list

The use of the Ti plasmid as a vector for the introduction of foreign DNA into plants

Author
Organization
Abstract
Agrobacterium tumefaciens is a Gram-negative bacterium with the unique capacity to induce neoplasmic transformations in dicotyledonous plants. Recently, both the mechanism and the biological significance of this transformation have been elucidated. Agrobacterium tumefaciens strains contain a large extrachromosomal DNA plasmid (the Ti-plasmid). This Ti-plasmid is responsible for the oncogenic properties of Agrobacterium strains. A particular segment of the Ti-plasmid, containing information determining the tumorous growth pattern and the synthesis of so-called "opines", e.g. "octopine" (N²-(D-1-carboxyethyl)-L-arginine) and "nopaline" (N²-(1,3-dicarboxypropyl)-L-arginine), is transferred and stably maintained and expressed in the transformed plant cells by bacterial plasmid DNA so that the transformed plant cell will produce and secrete into the medium amino acid derivatives (the opines) that Ti-plasmid carrying agrobacteria can selectively use as carbon and nitrogen sources. By in vivo genetic manipulations, we have recently been able to introduce a bacterial antibiotic resistance transposon, Tn7, in the Ti-DNA segment that is transferable to plant cells. In this way we hope to demonstrate that the Ti-plasmid can be used as a general vector for introducing "foreign" DNA into plants.

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 2.24 MB

Citation

Please use this url to cite or link to this publication:

MLA
De Beuckeleer, Marc, Marc De Block, Henri De Greve, et al. “The Use of the Ti Plasmid as a Vector for the Introduction of Foreign DNA into Plants.” Proceedings of the 4th International Conference on Plant Pathogenic Bacteria, Vol. 1. Ed. M Ridé. Angers, France: Institut National de la Recherche Agronomique (INRA), 1978. 115–126. Print.
APA
De Beuckeleer, M., De Block, M., De Greve, H., Depicker, A., De Vos, G., De Vos, R., De Wilde, M., et al. (1978). The use of the Ti plasmid as a vector for the introduction of foreign DNA into plants. In M. Ridé (Ed.), Proceedings of the 4th international conference on plant pathogenic bacteria, vol. 1 (pp. 115–126). Presented at the 4th International conference on Plant Pathogenic Bacteria, Angers, France: Institut National de la Recherche Agronomique (INRA).
Chicago author-date
De Beuckeleer, Marc, Marc De Block, Henri De Greve, Anna Depicker, Guido De Vos, Roger De Vos, Michel De Wilde, et al. 1978. “The Use of the Ti Plasmid as a Vector for the Introduction of Foreign DNA into Plants.” In Proceedings of the 4th International Conference on Plant Pathogenic Bacteria, Vol. 1, ed. M Ridé, 115–126. Angers, France: Institut National de la Recherche Agronomique (INRA).
Chicago author-date (all authors)
De Beuckeleer, Marc, Marc De Block, Henri De Greve, Anna Depicker, Guido De Vos, Roger De Vos, Michel De Wilde, Patrick Dhaese, Marie-Reine Dobbelaere, Gilbert Engler, Christiane Genetello, Jean-Pierre Hernalsteens, Marcella Holsters, Anni Jacobs, Eric Messens, Jeff Schell, Jef Seurinck, Ana Berta Silva, Eddy Van Haute, Marc Van Montagu, Françoise Van Vliet, Raimundo Villarroel-Mandiola, and Ivo Zaenen. 1978. “The Use of the Ti Plasmid as a Vector for the Introduction of Foreign DNA into Plants.” In Proceedings of the 4th International Conference on Plant Pathogenic Bacteria, Vol. 1, ed. M Ridé, 115–126. Angers, France: Institut National de la Recherche Agronomique (INRA).
Vancouver
1.
De Beuckeleer M, De Block M, De Greve H, Depicker A, De Vos G, De Vos R, et al. The use of the Ti plasmid as a vector for the introduction of foreign DNA into plants. In: Ridé M, editor. Proceedings of the 4th international conference on plant pathogenic bacteria, vol. 1. Angers, France: Institut National de la Recherche Agronomique (INRA); 1978. p. 115–26.
IEEE
[1]
M. De Beuckeleer et al., “The use of the Ti plasmid as a vector for the introduction of foreign DNA into plants,” in Proceedings of the 4th international conference on plant pathogenic bacteria, vol. 1, Angers, France, 1978, pp. 115–126.
@inproceedings{5671469,
  abstract     = {Agrobacterium tumefaciens is a Gram-negative bacterium with the unique capacity to induce neoplasmic transformations in dicotyledonous plants. Recently, both the mechanism and the biological significance of this transformation have been elucidated. Agrobacterium tumefaciens strains contain a large extrachromosomal DNA plasmid (the Ti-plasmid). This Ti-plasmid is responsible for the oncogenic properties of Agrobacterium strains. A particular segment of the Ti-plasmid, containing information determining the tumorous growth pattern and the synthesis of so-called "opines", e.g. "octopine" (N²-(D-1-carboxyethyl)-L-arginine) and "nopaline" (N²-(1,3-dicarboxypropyl)-L-arginine), is transferred and stably maintained and expressed in the transformed plant cells by bacterial plasmid DNA so that the transformed plant cell will produce and secrete into the medium amino acid derivatives (the opines) that Ti-plasmid carrying agrobacteria can selectively use as carbon and nitrogen sources. By in vivo genetic manipulations, we have recently been able to introduce a bacterial antibiotic resistance transposon, Tn7, in the Ti-DNA segment that is transferable to plant cells. In this way we hope to demonstrate that the Ti-plasmid can be used as a general vector for introducing "foreign" DNA into plants.},
  author       = {De Beuckeleer, Marc and De Block, Marc and De Greve, Henri and Depicker, Anna and De Vos, Guido and De Vos, Roger and De Wilde, Michel and Dhaese, Patrick and Dobbelaere, Marie-Reine and Engler, Gilbert and Genetello, Christiane and Hernalsteens, Jean-Pierre and Holsters, Marcella and Jacobs, Anni and Messens, Eric and Schell, Jeff and Seurinck, Jef and Silva, Ana Berta and Van Haute, Eddy and Van Montagu, Marc and Van Vliet, Françoise and Villarroel-Mandiola, Raimundo and Zaenen, Ivo},
  booktitle    = {Proceedings of the 4th international conference on plant pathogenic bacteria, vol. 1},
  editor       = {Ridé, M},
  language     = {eng},
  location     = {Angers, France},
  pages        = {115--126},
  publisher    = {Institut National de la Recherche Agronomique (INRA)},
  title        = {The use of the Ti plasmid as a vector for the introduction of foreign DNA into plants},
  year         = {1978},
}