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MRI-guided 3D conformal arc micro-irradiation of a F98 glioblastoma rat model using the Small Animal Radiation Research Platform (SARRP)

Julie Bolcaen, Benedicte Descamps UGent, Karel Deblaere UGent, Tom Boterberg UGent, GIORGIO HALLAERT, Caroline Van den Broecke UGent, Elke Decrock UGent, Anne Vral UGent, Luc Leybaert UGent, Christian Vanhove UGent, et al. (2014) JOURNAL OF NEURO-ONCOLOGY. 120(2). p.257-266
abstract
Current glioblastoma (GB) small animal models for cranial radiation therapy (RT) use simple single beam technologies, which differ from the advanced conformal image-guided radiation techniques used in clinical practice. This technological disparity presents a major disadvantage for the development of new therapeutic approaches. Hence, we established a F98 GB rat model using magnetic resonance imaging (MRI)-guided three-dimensional (3D)-conformal arc RT with the Small Animal Radiation Research Platform (SARRP). Ten Fischer rats were inoculated with F98 tumor cells. When the tumor reached a volume of approximately 27 mm(3) on T2-weighted MR images, the animals were randomized into a treatment group (n = 5) receiving RT and concomitant temozolomide, and a sham group (n = 5) receiving control injections. For the treated animals, contrast-enhanced T1-weighted MR images were acquired followed by a cone-beam computed tomography (CBCT) on the SARRP system. Both scans were co-registered; MRI was used to define the target whereas CBCT was used for calculating a dose plan (20 Gy, three non-coplanar arc beams, 3 x 3 mm collimator). Tumor volumes were evaluated on follow-up contrast-enhanced T1-weighted MR images. Verification of treatment accuracy with gamma H2AX immunohistochemical staining was performed. Tumors in the control animals showed rapid proliferation during follow-up, encompassing almost the entire right cerebral hemisphere at day 12-15. Treated animals showed no significant tumor growth from 2 to 9 days post RT. gamma H2AX results confirmed the accuracy of dose delivery. This model, which is quite similar to the approach in the clinic, is valid for combined RT and chemotherapy of GB in rats.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Conformal radiation therapy, F98 glioblastoma, MRI-guidance, TEMOZOLOMIDE, MULTIFORME, RECURRENCE, GLIOMA, BRAIN
journal title
JOURNAL OF NEURO-ONCOLOGY
J. Neuro-Oncol.
volume
120
issue
2
pages
257 - 266
Web of Science type
Article
Web of Science id
000344172600004
JCR category
CLINICAL NEUROLOGY
JCR impact factor
3.07 (2014)
JCR rank
59/192 (2014)
JCR quartile
2 (2014)
ISSN
0167-594X
DOI
10.1007/s11060-014-1552-9
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5666565
handle
http://hdl.handle.net/1854/LU-5666565
date created
2014-08-04 11:36:57
date last changed
2016-12-19 15:47:26
@article{5666565,
  abstract     = {Current glioblastoma (GB) small animal models for cranial radiation therapy (RT) use simple single beam technologies, which differ from the advanced conformal image-guided radiation techniques used in clinical practice. This technological disparity presents a major disadvantage for the development of new therapeutic approaches. Hence, we established a F98 GB rat model using magnetic resonance imaging (MRI)-guided three-dimensional (3D)-conformal arc RT with the Small Animal Radiation Research Platform (SARRP). Ten Fischer rats were inoculated with F98 tumor cells. When the tumor reached a volume of approximately 27 mm(3) on T2-weighted MR images, the animals were randomized into a treatment group (n = 5) receiving RT and concomitant temozolomide, and a sham group (n = 5) receiving control injections. For the treated animals, contrast-enhanced T1-weighted MR images were acquired followed by a cone-beam computed tomography (CBCT) on the SARRP system. Both scans were co-registered; MRI was used to define the target whereas CBCT was used for calculating a dose plan (20 Gy, three non-coplanar arc beams, 3 x 3 mm collimator). Tumor volumes were evaluated on follow-up contrast-enhanced T1-weighted MR images. Verification of treatment accuracy with gamma H2AX immunohistochemical staining was performed. Tumors in the control animals showed rapid proliferation during follow-up, encompassing almost the entire right cerebral hemisphere at day 12-15. Treated animals showed no significant tumor growth from 2 to 9 days post RT. gamma H2AX results confirmed the accuracy of dose delivery. This model, which is quite similar to the approach in the clinic, is valid for combined RT and chemotherapy of GB in rats.},
  author       = {Bolcaen, Julie and Descamps, Benedicte and Deblaere, Karel and Boterberg, Tom and HALLAERT, GIORGIO and Van den Broecke, Caroline and Decrock, Elke and Vral, Anne and Leybaert, Luc and Vanhove, Christian and Goethals, Ingeborg},
  issn         = {0167-594X},
  journal      = {JOURNAL OF NEURO-ONCOLOGY},
  keyword      = {Conformal radiation therapy,F98 glioblastoma,MRI-guidance,TEMOZOLOMIDE,MULTIFORME,RECURRENCE,GLIOMA,BRAIN},
  language     = {eng},
  number       = {2},
  pages        = {257--266},
  title        = {MRI-guided 3D conformal arc micro-irradiation of a F98 glioblastoma rat model using the Small Animal Radiation Research Platform (SARRP)},
  url          = {http://dx.doi.org/10.1007/s11060-014-1552-9},
  volume       = {120},
  year         = {2014},
}

Chicago
Bolcaen, Julie, Benedicte Descamps, Karel Deblaere, Tom Boterberg, GIORGIO HALLAERT, Caroline Van den Broecke, Elke Decrock, et al. 2014. “MRI-guided 3D Conformal Arc Micro-irradiation of a F98 Glioblastoma Rat Model Using the Small Animal Radiation Research Platform (SARRP).” Journal of Neuro-oncology 120 (2): 257–266.
APA
Bolcaen, J., Descamps, B., Deblaere, K., Boterberg, T., HALLAERT, G., Van den Broecke, C., Decrock, E., et al. (2014). MRI-guided 3D conformal arc micro-irradiation of a F98 glioblastoma rat model using the Small Animal Radiation Research Platform (SARRP). JOURNAL OF NEURO-ONCOLOGY, 120(2), 257–266.
Vancouver
1.
Bolcaen J, Descamps B, Deblaere K, Boterberg T, HALLAERT G, Van den Broecke C, et al. MRI-guided 3D conformal arc micro-irradiation of a F98 glioblastoma rat model using the Small Animal Radiation Research Platform (SARRP). JOURNAL OF NEURO-ONCOLOGY. 2014;120(2):257–66.
MLA
Bolcaen, Julie, Benedicte Descamps, Karel Deblaere, et al. “MRI-guided 3D Conformal Arc Micro-irradiation of a F98 Glioblastoma Rat Model Using the Small Animal Radiation Research Platform (SARRP).” JOURNAL OF NEURO-ONCOLOGY 120.2 (2014): 257–266. Print.