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Replacing serum in culture medium with albumin and insulin, transferrin and selenium is the key to successful bovine embryo development in individual culture

Eline Wydooghe (UGent) , Sonia Heras Garcia (UGent) , Jeroen Dewulf (UGent) , Sofie Piepers (UGent) , Etienne Van den Abbeel (UGent) , Petra De Sutter (UGent) , Leen Vandaele (UGent) and Ann Van Soom (UGent)
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Abstract
Individual culture of bovine embryos is usually associated with low blastocyst development. However, during preliminary experiments in our laboratory we observed high blastocyst development after individual embryo culture in a serum-free culture system. We therefore hypothesised that serum has a negative effect on embryos cultured individually whereas embryos in groups can counteract this. First, we determined whether the timing of removal of serum (during maturation or culture) had an influence on individual embryo development. The results clearly showed that removal of serum during embryo culture was the main contributing factor since high blastocyst development was observed after individual culture in synthetic oviductal fluid supplemented with bovine serum albumin (BSA) and insulin, transferrin and selenium (ITS), independent of the maturation medium. Second, we investigated whether an individual factor of the ITS supplement was essential for individual embryo development. We demonstrated that repeatable high blastocyst percentages were due to the synergistic effect of ITS. Finally, we investigated if a group-culture effect can still be observed under serum-free conditions. Group culture generated blastocysts with higher total cell numbers and less apoptosis. These data show that individual culture in serum-free conditions leads to high blastocyst development, but group culture still improves blastocyst quality.
Keywords
PLASMINOGEN, INVITRO, COMPETENCE, OOCYTES, VITRO-PRODUCED EMBRYOS, DEVELOPMENT IN-VITRO, PREIMPLANTATION EMBRYO, GROWTH-FACTORS, BLASTOCYSTS, semi-defined medium, in vitro embryo production, ITS, epidermal growth factor, autocrine factors, CUMULUS CELL COCULTURE, blastocyst quality

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Chicago
Wydooghe, Eline, Sonia Heras Garcia, Jeroen Dewulf, Sofie Piepers, Etienne Van den Abbeel, Petra De Sutter, Leen Vandaele, and Ann Van Soom. 2014. “Replacing Serum in Culture Medium with Albumin and Insulin, Transferrin and Selenium Is the Key to Successful Bovine Embryo Development in Individual Culture.” Reproduction Fertility and Development 26 (5): 717–724.
APA
Wydooghe, E., Heras Garcia, S., Dewulf, J., Piepers, S., Van den Abbeel, E., De Sutter, P., Vandaele, L., et al. (2014). Replacing serum in culture medium with albumin and insulin, transferrin and selenium is the key to successful bovine embryo development in individual culture. REPRODUCTION FERTILITY AND DEVELOPMENT, 26(5), 717–724.
Vancouver
1.
Wydooghe E, Heras Garcia S, Dewulf J, Piepers S, Van den Abbeel E, De Sutter P, et al. Replacing serum in culture medium with albumin and insulin, transferrin and selenium is the key to successful bovine embryo development in individual culture. REPRODUCTION FERTILITY AND DEVELOPMENT. 2014;26(5):717–24.
MLA
Wydooghe, Eline, Sonia Heras Garcia, Jeroen Dewulf, et al. “Replacing Serum in Culture Medium with Albumin and Insulin, Transferrin and Selenium Is the Key to Successful Bovine Embryo Development in Individual Culture.” REPRODUCTION FERTILITY AND DEVELOPMENT 26.5 (2014): 717–724. Print.
@article{5660678,
  abstract     = {Individual culture of bovine embryos is usually associated with low blastocyst development. However, during preliminary experiments in our laboratory we observed high blastocyst development after individual embryo culture in a serum-free culture system. We therefore hypothesised that serum has a negative effect on embryos cultured individually whereas embryos in groups can counteract this. First, we determined whether the timing of removal of serum (during maturation or culture) had an influence on individual embryo development. The results clearly showed that removal of serum during embryo culture was the main contributing factor since high blastocyst development was observed after individual culture in synthetic oviductal fluid supplemented with bovine serum albumin (BSA) and insulin, transferrin and selenium (ITS), independent of the maturation medium. Second, we investigated whether an individual factor of the ITS supplement was essential for individual embryo development. We demonstrated that repeatable high blastocyst percentages were due to the synergistic effect of ITS. Finally, we investigated if a group-culture effect can still be observed under serum-free conditions. Group culture generated blastocysts with higher total cell numbers and less apoptosis. These data show that individual culture in serum-free conditions leads to high blastocyst development, but group culture still improves blastocyst quality.},
  author       = {Wydooghe, Eline and Heras Garcia, Sonia and Dewulf, Jeroen and Piepers, Sofie and Van den Abbeel, Etienne and De Sutter, Petra and Vandaele, Leen and Van Soom, Ann},
  issn         = {1031-3613},
  journal      = {REPRODUCTION FERTILITY AND DEVELOPMENT},
  language     = {eng},
  number       = {5},
  pages        = {717--724},
  title        = {Replacing serum in culture medium with albumin and insulin, transferrin and selenium is the key to successful bovine embryo development in individual culture},
  url          = {http://dx.doi.org/10.1071/RD13043},
  volume       = {26},
  year         = {2014},
}

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