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Vitrification of human blastocysts previously cryopreserved by slow controlled-rate freezing at the cleavage stage

Sylvie Lierman (UGent) , Etienne Van den Abbeel (UGent) and Petra De Sutter (UGent)
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Organization
Abstract
Purpose: The objective of this study was to evaluate the efficiency of vitrified blastocysts derived from frozenthawed cleavage stage embryos in terms of morphological survival and re-expansion status of the blastocoelic cavity. Results: After warming 162 blastocysts derived from fresh embryos (= control group) and 90 blastocysts from frozenthawed cleavage stage embryos (= study group) and after 2–3 h of in vitro culture the percentage of blastocysts with morphological survival was not different between the two groups. After 24 h of in vitro culture, the percentage of fully expanded, hatching or hatched blastocysts was not different between both groups. Conclusion(s): The results show that blastocysts derived from frozen-thawed cleavage stage embryos can be cryopreserved successfully a second time by vitrification method. Recryopreservation by vitrification still needs to be approached with some caution because little data on long term safety of multiple freezing is available.
Keywords
RE-VITRIFICATION, HUMAN EMBRYOS, REFROZEN, THAWED TWICE, IN-VITRO DEVELOPMENT, Morphological survival, Human blastocyst, Vitrification, Re-cryopreservation, BIRTH, METAANALYSIS, PRONUCLEAR, IMPACT, FROZEN

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MLA
Lierman, Sylvie, Etienne Van den Abbeel, and Petra De Sutter. “Vitrification of Human Blastocysts Previously Cryopreserved by Slow Controlled-rate Freezing at the Cleavage Stage.” JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 31.4 (2014): 447–451. Print.
APA
Lierman, Sylvie, Van den Abbeel, E., & De Sutter, P. (2014). Vitrification of human blastocysts previously cryopreserved by slow controlled-rate freezing at the cleavage stage. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 31(4), 447–451.
Chicago author-date
Lierman, Sylvie, Etienne Van den Abbeel, and Petra De Sutter. 2014. “Vitrification of Human Blastocysts Previously Cryopreserved by Slow Controlled-rate Freezing at the Cleavage Stage.” Journal of Assisted Reproduction and Genetics 31 (4): 447–451.
Chicago author-date (all authors)
Lierman, Sylvie, Etienne Van den Abbeel, and Petra De Sutter. 2014. “Vitrification of Human Blastocysts Previously Cryopreserved by Slow Controlled-rate Freezing at the Cleavage Stage.” Journal of Assisted Reproduction and Genetics 31 (4): 447–451.
Vancouver
1.
Lierman S, Van den Abbeel E, De Sutter P. Vitrification of human blastocysts previously cryopreserved by slow controlled-rate freezing at the cleavage stage. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS. 2014;31(4):447–51.
IEEE
[1]
S. Lierman, E. Van den Abbeel, and P. De Sutter, “Vitrification of human blastocysts previously cryopreserved by slow controlled-rate freezing at the cleavage stage,” JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol. 31, no. 4, pp. 447–451, 2014.
@article{5660657,
  abstract     = {Purpose: The objective of this study was to evaluate the efficiency of vitrified blastocysts derived from frozenthawed cleavage stage embryos in terms of morphological survival and re-expansion status of the blastocoelic cavity.
Results: After warming 162 blastocysts derived from fresh embryos (= control group) and 90 blastocysts from frozenthawed cleavage stage embryos (= study group) and after 2–3 h of in vitro culture the percentage of blastocysts with morphological survival was not different between the two groups. After 24 h of in vitro culture, the percentage of fully expanded, hatching or hatched blastocysts was not different between both groups.
Conclusion(s): The results show that blastocysts derived from frozen-thawed cleavage stage embryos can be cryopreserved successfully a second time by vitrification method. Recryopreservation by vitrification still needs to be approached with some caution because little data on long term safety of multiple freezing is available.},
  author       = {Lierman, Sylvie and Van den Abbeel, Etienne and De Sutter, Petra},
  issn         = {1058-0468},
  journal      = {JOURNAL OF ASSISTED REPRODUCTION AND GENETICS},
  keywords     = {RE-VITRIFICATION,HUMAN EMBRYOS,REFROZEN,THAWED TWICE,IN-VITRO DEVELOPMENT,Morphological survival,Human blastocyst,Vitrification,Re-cryopreservation,BIRTH,METAANALYSIS,PRONUCLEAR,IMPACT,FROZEN},
  language     = {eng},
  number       = {4},
  pages        = {447--451},
  title        = {Vitrification of human blastocysts previously cryopreserved by slow controlled-rate freezing at the cleavage stage},
  url          = {http://dx.doi.org/10.1007/s10815-013-0164-1},
  volume       = {31},
  year         = {2014},
}

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