Advanced search
1 file | 1.01 MB

A cryptic cytoplasmic male sterility unveils a possible gynodioecious past for Arabidopsis thaliana

(2013) PLOS ONE. 8(4).
Author
Organization
Abstract
Gynodioecy, the coexistence of hermaphrodites and females (i.e. male-sterile plants) in natural plant populations, most often results from polymorphism at genetic loci involved in a particular interaction between the nuclear and cytoplasmic genetic compartments (cytonuclear epistasis): cytoplasmic male sterility (CMS). Although CMS clearly contributes to the coevolution of involved nuclear loci and cytoplasmic genomes in gynodioecious species, the occurrence of CMS genetic factors in the absence of sexual polymorphism (cryptic CMS) is not easily detected and rarely taken in consideration. We found cryptic CMS in the model plant Arabidopsis thaliana after crossing distantly related accessions, Sha and Mr-0. Male sterility resulted from an interaction between the Sha cytoplasm and two Mr-0 genomic regions located on chromosome 1 and chromosome 3. Additional accessions with either nuclear sterility maintainers or sterilizing cytoplasms were identified from crosses with either Sha or Mr-0. By comparing two very closely related cytoplasms with different male-sterility inducing abilities, we identified a novel mitochondrial ORF, named orf117Sha, that is most likely the sterilizing factor of the Sha cytoplasm. The presence of orf117Sha was investigated in worldwide natural accessions. It was found mainly associated with a single chlorotype in accessions belonging to a clade predominantly originating from Central Asia. More than one-third of accessions from this clade carried orf117Sha, indicating that the sterilizing-inducing cytoplasm had spread in this lineage. We also report the coexistence of the sterilizing cytoplasm with a non-sterilizing cytoplasm at a small, local scale in a natural population; in addition a correlation between cytotype and nuclear haplotype was detected in this population. Our results suggest that this CMS system induced sexual polymorphism in A. thaliana populations, at the time when the species was mainly outcrossing.
Keywords
MITOCHONDRIAL GENOMES, RAPHANUS-RAPHANISTRUM, SELF-COMPATIBILITY, NUCLEAR, EVOLUTION, FERTILITY, DNA, INCOMPATIBILITY, POPULATIONS, EXPRESSION

Downloads

  • Gobron et al. 2013 PLoS ONE 8 e62450.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.01 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Gobron, Nicolas, Cezary Waszczak, Matthieu Simon, Sophie Hiard, Stéphane Boivin, Delphine Charif, Aloïse Ducamp, Estelle Wenes, and Françoise Budar. 2013. “A Cryptic Cytoplasmic Male Sterility Unveils a Possible Gynodioecious Past for Arabidopsis Thaliana.” Plos One 8 (4).
APA
Gobron, N., Waszczak, C., Simon, M., Hiard, S., Boivin, S., Charif, D., Ducamp, A., et al. (2013). A cryptic cytoplasmic male sterility unveils a possible gynodioecious past for Arabidopsis thaliana. PLOS ONE, 8(4).
Vancouver
1.
Gobron N, Waszczak C, Simon M, Hiard S, Boivin S, Charif D, et al. A cryptic cytoplasmic male sterility unveils a possible gynodioecious past for Arabidopsis thaliana. PLOS ONE. 2013;8(4).
MLA
Gobron, Nicolas, Cezary Waszczak, Matthieu Simon, et al. “A Cryptic Cytoplasmic Male Sterility Unveils a Possible Gynodioecious Past for Arabidopsis Thaliana.” PLOS ONE 8.4 (2013): n. pag. Print.
@article{5652509,
  abstract     = {Gynodioecy, the coexistence of hermaphrodites and females (i.e. male-sterile plants) in natural plant populations, most often results from polymorphism at genetic loci involved in a particular interaction between the nuclear and cytoplasmic genetic compartments (cytonuclear epistasis): cytoplasmic male sterility (CMS). Although CMS clearly contributes to the coevolution of involved nuclear loci and cytoplasmic genomes in gynodioecious species, the occurrence of CMS genetic factors in the absence of sexual polymorphism (cryptic CMS) is not easily detected and rarely taken in consideration. We found cryptic CMS in the model plant Arabidopsis thaliana after crossing distantly related accessions, Sha and Mr-0. Male sterility resulted from an interaction between the Sha cytoplasm and two Mr-0 genomic regions located on chromosome 1 and chromosome 3. Additional accessions with either nuclear sterility maintainers or sterilizing cytoplasms were identified from crosses with either Sha or Mr-0. By comparing two very closely related cytoplasms with different male-sterility inducing abilities, we identified a novel mitochondrial ORF, named orf117Sha, that is most likely the sterilizing factor of the Sha cytoplasm. The presence of orf117Sha was investigated in worldwide natural accessions. It was found mainly associated with a single chlorotype in accessions belonging to a clade predominantly originating from Central Asia. More than one-third of accessions from this clade carried orf117Sha, indicating that the sterilizing-inducing cytoplasm had spread in this lineage. We also report the coexistence of the sterilizing cytoplasm with a non-sterilizing cytoplasm at a small, local scale in a natural population; in addition a correlation between cytotype and nuclear haplotype was detected in this population. Our results suggest that this CMS system induced sexual polymorphism in A. thaliana populations, at the time when the species was mainly outcrossing.},
  articleno    = {e62450},
  author       = {Gobron, Nicolas and Waszczak, Cezary and Simon, Matthieu and Hiard, Sophie and Boivin, St{\'e}phane and Charif, Delphine and Ducamp, Alo{\"i}se and Wenes, Estelle and Budar, Fran\c{c}oise},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  language     = {eng},
  number       = {4},
  pages        = {13},
  title        = {A cryptic cytoplasmic male sterility unveils a possible gynodioecious past for Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1371/journal.pone.0062450},
  volume       = {8},
  year         = {2013},
}

Altmetric
View in Altmetric
Web of Science
Times cited: