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Mapping of the ms8 male sterility gene in sweet pepper (Capsicum annuum L.) on the chromosome P4 using PCR-based markers useful for breeding programmes

(2012) EUPHYTICA. 186(2). p.453-461
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Abstract
The nuclear male sterility gene ms8 is expected to facilitate the production of sweet pepper (Capsicum annuum L.) hybrids as it provides means for hybridization without the labor-intensive hand emasculation of female inbred lines. The development of molecular markers linked to ms8 locus will help the breeding practice for the selection of hybrid parental lines. In this study, F-2 population resulting from a cross between the sweet pepper male sterile line 320 and the male fertile variety Elf was used to identify DNA markers linked to the ms8 locus. With the use of RAPD-BSA technique, seven markers linked to the ms8 locus were found. Four of them were converted into SCAR markers. In addition, two COSII/CAPS markers linked to the ms8 locus were identified. Comparative mapping with reference pepper maps indicated that the ms8 locus is located on the lower arm of the pepper chromosome P4. Identified markers are useful for molecular breeding, however, at present markers tightly linked to ms8 locus are still lacking. Identification of molecular markers linked to the ms8 locus and determination of its chromosomal localization are useful for fine mapping and also provide the perspective for ms8 gene cloning.
Keywords
Pepper, Genic male sterility, PCR-based markers, Genetic mapping, ms8, Chromosome P4, POLYGENIC RESISTANCE, QTL ANALYSIS, IDENTIFICATION, SOLANACEAE, TOMATO, LOCI, MAP

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Citation

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MLA
Bartoszewski, Grzegorz, Cezary Waszczak, Piotr Gawroński, et al. “Mapping of the Ms8 Male Sterility Gene in Sweet Pepper (Capsicum Annuum L.) on the Chromosome P4 Using PCR-based Markers Useful for Breeding Programmes.” EUPHYTICA 186.2 (2012): 453–461. Print.
APA
Bartoszewski, G., Waszczak, C., Gawroński, P., Stępień, I., Bolibok-Brągoszewska, H., Palloix, A., Lefebvre, V., et al. (2012). Mapping of the ms8 male sterility gene in sweet pepper (Capsicum annuum L.) on the chromosome P4 using PCR-based markers useful for breeding programmes. EUPHYTICA, 186(2), 453–461.
Chicago author-date
Bartoszewski, Grzegorz, Cezary Waszczak, Piotr Gawroński, Iwona Stępień, Hanna Bolibok-Brągoszewska, Alain Palloix, Véronique Lefebvre, Aleksandra Korzeniewska, and Katarzyna Niemirowicz-Szczytt. 2012. “Mapping of the Ms8 Male Sterility Gene in Sweet Pepper (Capsicum Annuum L.) on the Chromosome P4 Using PCR-based Markers Useful for Breeding Programmes.” Euphytica 186 (2): 453–461.
Chicago author-date (all authors)
Bartoszewski, Grzegorz, Cezary Waszczak, Piotr Gawroński, Iwona Stępień, Hanna Bolibok-Brągoszewska, Alain Palloix, Véronique Lefebvre, Aleksandra Korzeniewska, and Katarzyna Niemirowicz-Szczytt. 2012. “Mapping of the Ms8 Male Sterility Gene in Sweet Pepper (Capsicum Annuum L.) on the Chromosome P4 Using PCR-based Markers Useful for Breeding Programmes.” Euphytica 186 (2): 453–461.
Vancouver
1.
Bartoszewski G, Waszczak C, Gawroński P, Stępień I, Bolibok-Brągoszewska H, Palloix A, et al. Mapping of the ms8 male sterility gene in sweet pepper (Capsicum annuum L.) on the chromosome P4 using PCR-based markers useful for breeding programmes. EUPHYTICA. 2012;186(2):453–61.
IEEE
[1]
G. Bartoszewski et al., “Mapping of the ms8 male sterility gene in sweet pepper (Capsicum annuum L.) on the chromosome P4 using PCR-based markers useful for breeding programmes,” EUPHYTICA, vol. 186, no. 2, pp. 453–461, 2012.
@article{5652488,
  abstract     = {The nuclear male sterility gene ms8 is expected to facilitate the production of sweet pepper (Capsicum annuum L.) hybrids as it provides means for hybridization without the labor-intensive hand emasculation of female inbred lines. The development of molecular markers linked to ms8 locus will help the breeding practice for the selection of hybrid parental lines. In this study, F-2 population resulting from a cross between the sweet pepper male sterile line 320 and the male fertile variety Elf was used to identify DNA markers linked to the ms8 locus. With the use of RAPD-BSA technique, seven markers linked to the ms8 locus were found. Four of them were converted into SCAR markers. In addition, two COSII/CAPS markers linked to the ms8 locus were identified. Comparative mapping with reference pepper maps indicated that the ms8 locus is located on the lower arm of the pepper chromosome P4. Identified markers are useful for molecular breeding, however, at present markers tightly linked to ms8 locus are still lacking. Identification of molecular markers linked to the ms8 locus and determination of its chromosomal localization are useful for fine mapping and also provide the perspective for ms8 gene cloning.},
  author       = {Bartoszewski, Grzegorz and Waszczak, Cezary and Gawroński, Piotr and Stępień, Iwona and Bolibok-Brągoszewska, Hanna and Palloix, Alain and Lefebvre, Véronique and Korzeniewska, Aleksandra and Niemirowicz-Szczytt, Katarzyna},
  issn         = {0014-2336},
  journal      = {EUPHYTICA},
  keywords     = {Pepper,Genic male sterility,PCR-based markers,Genetic mapping,ms8,Chromosome P4,POLYGENIC RESISTANCE,QTL ANALYSIS,IDENTIFICATION,SOLANACEAE,TOMATO,LOCI,MAP},
  language     = {eng},
  number       = {2},
  pages        = {453--461},
  title        = {Mapping of the ms8 male sterility gene in sweet pepper (Capsicum annuum L.) on the chromosome P4 using PCR-based markers useful for breeding programmes},
  url          = {http://dx.doi.org/10.1007/s10681-012-0637-9},
  volume       = {186},
  year         = {2012},
}

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