Ghent University Academic Bibliography

Advanced

Giardia duodenalis stimulates partial maturation of bovine dendritic cells associated with altered cytokine secretion and induction of T-cell proliferation

Grietje Grit, Bert Devriendt UGent, Stefanie Van Coppernolle UGent, Thomas Geurden UGent, J Hope, Jozef Vercruysse UGent, Eric Cox UGent, Peter Geldhof UGent and Edwin Claerebout UGent (2014) PARASITE IMMUNOLOGY. 36(4). p.157-169
abstract
Giardia duodenalis is an important intestinal parasite in animals and humans. The role of dendritic cells (DC) in the initiation of the immune response against G.duodenalis is poorly documented. The aim of this study was to test the hypothesis that G.duodenalis interferes with bovine DC function. Therefore, the effect of trophozoites and excretion/secretion products on bovine monocyte-derived dendritic cells (MoDC) was investigated. We assessed MoDC maturation and cytokine production of G.duodenalis-stimulated MoDC and the ability of these MoDC to take up antigen and induce lymphocyte proliferation. Little or no upregulation of maturation markers CD40 and CD80 was measured, but MHCII expression was increased after stimulation with low parasite concentrations. A dose-dependent decrease in ovalbumin uptake was observed in G.duodenalis-stimulated MoDC. In addition, stimulated MoDC induced proliferation of CD3(-), -T-cells and TCR+ CD4(+) and CD8(+) T-cells. Increased transcription of TGF- was shown in CD4(+) T cells, and increased TNF-, TGF-, IL-10 and IL-4 were seen in -T-cells. We found no evidence that G.duodenalis has a regulatory or inhibitory effect on bovine MoDC. MoDC stimulated with G.duodenalis are functionally active and able to induce proliferation of T cells that produce both pro- and anti-inflammatory cytokines.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
maturation, T-cell proliferation, Giardia duodenalis, cattle, dendritic cells, EXPRESSION, CALVES, CRYPTOSPORIDIUM, LYMPHOCYTES, INFECTIONS, TOLERANCE, MONOCYTE, RESPIRATORY SYNCYTIAL VIRUS, IMMUNE-RESPONSE, LAMBLIA
journal title
PARASITE IMMUNOLOGY
Parasite Immunol.
volume
36
issue
4
pages
157 - 169
Web of Science type
Article
Web of Science id
000332324700003
JCR category
PARASITOLOGY
JCR impact factor
2.143 (2014)
JCR rank
14/36 (2014)
JCR quartile
2 (2014)
ISSN
0141-9838
DOI
10.1111/pim.12095
project
IWT
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5644621
handle
http://hdl.handle.net/1854/LU-5644621
date created
2014-07-09 09:48:10
date last changed
2016-12-19 15:39:17
@article{5644621,
  abstract     = {Giardia duodenalis is an important intestinal parasite in animals and humans. The role of dendritic cells (DC) in the initiation of the immune response against G.duodenalis is poorly documented. The aim of this study was to test the hypothesis that G.duodenalis interferes with bovine DC function. Therefore, the effect of trophozoites and excretion/secretion products on bovine monocyte-derived dendritic cells (MoDC) was investigated. We assessed MoDC maturation and cytokine production of G.duodenalis-stimulated MoDC and the ability of these MoDC to take up antigen and induce lymphocyte proliferation. Little or no upregulation of maturation markers CD40 and CD80 was measured, but MHCII expression was increased after stimulation with low parasite concentrations. A dose-dependent decrease in ovalbumin uptake was observed in G.duodenalis-stimulated MoDC. In addition, stimulated MoDC induced proliferation of CD3(-), -T-cells and TCR+ CD4(+) and CD8(+) T-cells. Increased transcription of TGF- was shown in CD4(+) T cells, and increased TNF-, TGF-, IL-10 and IL-4 were seen in -T-cells. We found no evidence that G.duodenalis has a regulatory or inhibitory effect on bovine MoDC. MoDC stimulated with G.duodenalis are functionally active and able to induce proliferation of T cells that produce both pro- and anti-inflammatory cytokines.},
  author       = {Grit, Grietje and Devriendt, Bert and Van Coppernolle, Stefanie and Geurden, Thomas and Hope, J and Vercruysse, Jozef and Cox, Eric and Geldhof, Peter and Claerebout, Edwin},
  issn         = {0141-9838},
  journal      = {PARASITE IMMUNOLOGY},
  keyword      = {maturation,T-cell proliferation,Giardia duodenalis,cattle,dendritic cells,EXPRESSION,CALVES,CRYPTOSPORIDIUM,LYMPHOCYTES,INFECTIONS,TOLERANCE,MONOCYTE,RESPIRATORY SYNCYTIAL VIRUS,IMMUNE-RESPONSE,LAMBLIA},
  language     = {eng},
  number       = {4},
  pages        = {157--169},
  title        = {Giardia duodenalis stimulates partial maturation of bovine dendritic cells associated with altered cytokine secretion and induction of T-cell proliferation},
  url          = {http://dx.doi.org/10.1111/pim.12095},
  volume       = {36},
  year         = {2014},
}

Chicago
Grit, Grietje, Bert Devriendt, Stefanie Van Coppernolle, Thomas Geurden, J Hope, Jozef Vercruysse, Eric Cox, Peter Geldhof, and Edwin Claerebout. 2014. “Giardia Duodenalis Stimulates Partial Maturation of Bovine Dendritic Cells Associated with Altered Cytokine Secretion and Induction of T-cell Proliferation.” Parasite Immunology 36 (4): 157–169.
APA
Grit, G., Devriendt, B., Van Coppernolle, S., Geurden, T., Hope, J., Vercruysse, J., Cox, E., et al. (2014). Giardia duodenalis stimulates partial maturation of bovine dendritic cells associated with altered cytokine secretion and induction of T-cell proliferation. PARASITE IMMUNOLOGY, 36(4), 157–169.
Vancouver
1.
Grit G, Devriendt B, Van Coppernolle S, Geurden T, Hope J, Vercruysse J, et al. Giardia duodenalis stimulates partial maturation of bovine dendritic cells associated with altered cytokine secretion and induction of T-cell proliferation. PARASITE IMMUNOLOGY. 2014;36(4):157–69.
MLA
Grit, Grietje, Bert Devriendt, Stefanie Van Coppernolle, et al. “Giardia Duodenalis Stimulates Partial Maturation of Bovine Dendritic Cells Associated with Altered Cytokine Secretion and Induction of T-cell Proliferation.” PARASITE IMMUNOLOGY 36.4 (2014): 157–169. Print.