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Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids

Dajo Smet UGent, Petra Zadnikova UGent, Filip Vandenbussche UGent, Eva Benkova and Dominique Van Der Straeten UGent (2014) NEW PHYTOLOGIST. 202(4). p.1398-1411
abstract
Germination of Arabidopsis seeds in darkness induces apical hook development, based on a tightly regulated differential growth coordinated by a multiple hormone cross-talk. Here, we endeavoured to clarify the function of brassinosteroids (BRs) and cross-talk with ethylene in hook development. An automated infrared imaging system was developed to study the kinetics of hook development in etiolated Arabidopsis seedlings. To ascertain the photomorphogenic control of hook opening, the system was equipped with an automatic light dimmer. We demonstrate that ethylene and BRs are indispensable for hook formation and maintenance. Ethylene regulation of hook formation functions partly through BRs, with BR feedback inhibition of ethylene action. Conversely, BR-mediated extension of hook maintenance functions partly through ethylene. Furthermore, we revealed that a short light pulse is sufficient to induce rapid hook opening. Our dynamic infrared imaging system allows high-resolution, kinetic imaging of up to 112 seedlings in a single experimental run. At this high throughput, it is ideally suited to rapidly gain insight in pathway networks. We demonstrate that BRs and ethylene cooperatively regulate apical hook development in a phase-dependent manner. Furthermore, we show that light is a predominant regulator of hook opening, inhibiting ethylene- and BR-mediated postponement of hook opening.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
cross-talk, kinematics, ethylene, infrared imaging, MEDIATED DEGRADATION, REPRESS PHOTOMORPHOGENESIS, RED-LIGHT, PLANT-GROWTH, CELL ELONGATION, KINETIC-ANALYSIS, HYPOCOTYL GROWTH, THALIANA SEEDLINGS, PROMOTING STEROIDAL LACTONE, ETHYLENE SIGNAL-TRANSDUCTION, light, apical hook, brassinosteroids (BRs), Arabidopsis thaliana
journal title
NEW PHYTOLOGIST
New Phytol.
volume
202
issue
4
pages
1398 - 1411
Web of Science type
Article
Web of Science id
000335470200029
JCR category
PLANT SCIENCES
JCR impact factor
7.672 (2014)
JCR rank
6/204 (2014)
JCR quartile
1 (2014)
ISSN
0028-646X
DOI
10.1111/nph.12751
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5644509
handle
http://hdl.handle.net/1854/LU-5644509
date created
2014-07-09 09:31:03
date last changed
2016-12-19 15:47:25
@article{5644509,
  abstract     = {Germination of Arabidopsis seeds in darkness induces apical hook development, based on a tightly regulated differential growth coordinated by a multiple hormone cross-talk. Here, we endeavoured to clarify the function of brassinosteroids (BRs) and cross-talk with ethylene in hook development. An automated infrared imaging system was developed to study the kinetics of hook development in etiolated Arabidopsis seedlings. To ascertain the photomorphogenic control of hook opening, the system was equipped with an automatic light dimmer. We demonstrate that ethylene and BRs are indispensable for hook formation and maintenance. Ethylene regulation of hook formation functions partly through BRs, with BR feedback inhibition of ethylene action. Conversely, BR-mediated extension of hook maintenance functions partly through ethylene. Furthermore, we revealed that a short light pulse is sufficient to induce rapid hook opening. Our dynamic infrared imaging system allows high-resolution, kinetic imaging of up to 112 seedlings in a single experimental run. At this high throughput, it is ideally suited to rapidly gain insight in pathway networks. We demonstrate that BRs and ethylene cooperatively regulate apical hook development in a phase-dependent manner. Furthermore, we show that light is a predominant regulator of hook opening, inhibiting ethylene- and BR-mediated postponement of hook opening.},
  author       = {Smet, Dajo and Zadnikova, Petra and Vandenbussche, Filip and Benkova, Eva and Van Der Straeten, Dominique},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  keyword      = {cross-talk,kinematics,ethylene,infrared imaging,MEDIATED DEGRADATION,REPRESS PHOTOMORPHOGENESIS,RED-LIGHT,PLANT-GROWTH,CELL ELONGATION,KINETIC-ANALYSIS,HYPOCOTYL GROWTH,THALIANA SEEDLINGS,PROMOTING STEROIDAL LACTONE,ETHYLENE SIGNAL-TRANSDUCTION,light,apical hook,brassinosteroids (BRs),Arabidopsis thaliana},
  language     = {eng},
  number       = {4},
  pages        = {1398--1411},
  title        = {Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids},
  url          = {http://dx.doi.org/10.1111/nph.12751},
  volume       = {202},
  year         = {2014},
}

Chicago
Smet, Dajo, Petra Zadnikova, Filip Vandenbussche, Eva Benkova, and Dominique Van Der Straeten. 2014. “Dynamic Infrared Imaging Analysis of Apical Hook Development in Arabidopsis: The Case of Brassinosteroids.” New Phytologist 202 (4): 1398–1411.
APA
Smet, Dajo, Zadnikova, P., Vandenbussche, F., Benkova, E., & Van Der Straeten, D. (2014). Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids. NEW PHYTOLOGIST, 202(4), 1398–1411.
Vancouver
1.
Smet D, Zadnikova P, Vandenbussche F, Benkova E, Van Der Straeten D. Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids. NEW PHYTOLOGIST. 2014;202(4):1398–411.
MLA
Smet, Dajo, Petra Zadnikova, Filip Vandenbussche, et al. “Dynamic Infrared Imaging Analysis of Apical Hook Development in Arabidopsis: The Case of Brassinosteroids.” NEW PHYTOLOGIST 202.4 (2014): 1398–1411. Print.