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Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens

(2014) JOURNAL OF APPLIED MICROBIOLOGY. 116(5). p.1084-1093
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Abstract
Aims: To explore antibacterial activities of coagulase-negative staphylococci (CoNS) from teat apices of dairy cows towards mastitis-causing pathogens. Methods and Results: Of 254 CoNS, 38 displayed bacteriocin-like activity after a first screening. Seven of these strains displayed activity against at least one mastitis-related pathogen (Streptococcus uberis, Streptococcus dysgalactiae and Staphylococcus aureus). Staphylococcus chromogenes L217 displayed the strongest inhibitory effect, being active against all tested mastitis-related pathogens and most tested CoNS. Based on cation exchange and reversed-phase chromatography, in addition to N-terminal Edman degradation and PCR, the antibacterial peptide was identified as a nukacin-type bacteriocin and named nukacin L217. Although staphylococcal bacteriocins are generally found in the cell-free supernatants of liquid cultures, Staph.chromogenes L217 only led to detectable activity when grown on agar medium. Conclusions: Bacteriocin-like activities are not uncommon among CoNS from teat apices and may inhibit mastitis-causing pathogens, as found for nukacin L217 production by Staph.chromogenes L217. Significance and Impact of the Study Nukacin L217 is the first identified bacteriocin of the species Staph.chromogenes and displays unusual production kinetics, that is, requiring surface growth of its producer. The fact that nukacins are produced by different CoNS species suggests a role in the teat skin ecosystem.
Keywords
AUREUS, MILK, HEIFERS, ANTIMICROBIAL PEPTIDES, EARLY LACTATION, 1ST LACTATION, NUKACIN ISK-1, DAIRY-COWS, INTRAMAMMARY INFECTIONS, mastitis, bacteriocin, Staphylococcus, udder health, LACTIC-ACID BACTERIA

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Chicago
Braem, G, B Stijlemans, W Van Haken, Sarne De Vliegher, L De Vuyst, and F Leroy. 2014. “Antibacterial Activities of Coagulase-negative Staphylococci from Bovine Teat Apex Skin and Their Inhibitory Effect on Mastitis-related Pathogens.” Journal of Applied Microbiology 116 (5): 1084–1093.
APA
Braem, G, Stijlemans, B., Van Haken, W., De Vliegher, S., De Vuyst, L., & Leroy, F. (2014). Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens. JOURNAL OF APPLIED MICROBIOLOGY, 116(5), 1084–1093.
Vancouver
1.
Braem G, Stijlemans B, Van Haken W, De Vliegher S, De Vuyst L, Leroy F. Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens. JOURNAL OF APPLIED MICROBIOLOGY. 2014;116(5):1084–93.
MLA
Braem, G, B Stijlemans, W Van Haken, et al. “Antibacterial Activities of Coagulase-negative Staphylococci from Bovine Teat Apex Skin and Their Inhibitory Effect on Mastitis-related Pathogens.” JOURNAL OF APPLIED MICROBIOLOGY 116.5 (2014): 1084–1093. Print.
@article{5640193,
  abstract     = {Aims: To explore antibacterial activities of coagulase-negative staphylococci (CoNS) from teat apices of dairy cows towards mastitis-causing pathogens.
Methods and Results: Of 254 CoNS, 38 displayed bacteriocin-like activity after a first screening. Seven of these strains displayed activity against at least one mastitis-related pathogen (Streptococcus uberis, Streptococcus dysgalactiae and Staphylococcus aureus). Staphylococcus chromogenes L217 displayed the strongest inhibitory effect, being active against all tested mastitis-related pathogens and most tested CoNS. Based on cation exchange and reversed-phase chromatography, in addition to N-terminal Edman degradation and PCR, the antibacterial peptide was identified as a nukacin-type bacteriocin and named nukacin L217. Although staphylococcal bacteriocins are generally found in the cell-free supernatants of liquid cultures, Staph.chromogenes L217 only led to detectable activity when grown on agar medium.
Conclusions: Bacteriocin-like activities are not uncommon among CoNS from teat apices and may inhibit mastitis-causing pathogens, as found for nukacin L217 production by Staph.chromogenes L217. Significance and Impact of the Study Nukacin L217 is the first identified bacteriocin of the species Staph.chromogenes and displays unusual production kinetics, that is, requiring surface growth of its producer. The fact that nukacins are produced by different CoNS species suggests a role in the teat skin ecosystem.},
  author       = {Braem, G and Stijlemans, B and Van Haken, W and De Vliegher, Sarne and De Vuyst, L and Leroy, F},
  issn         = {1364-5072},
  journal      = {JOURNAL OF APPLIED MICROBIOLOGY},
  language     = {eng},
  number       = {5},
  pages        = {1084--1093},
  title        = {Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens},
  url          = {http://dx.doi.org/10.1111/jam.12447},
  volume       = {116},
  year         = {2014},
}

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