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Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection

Belgacem Mihi, Frederik Van Meulder UGent, Manuela Rinaldi UGent, Stefanie Van Coppernolle UGent, Koen Chiers UGent, Wim Van Den Broeck UGent, Bruno Goddeeris, Jozef Vercruysse UGent, Edwin Claerebout UGent and Peter Geldhof UGent (2013) VETERINARY RESEARCH. 44.
abstract
Infections in cattle with the gastric nematode Ostertagia ostertagi are associated with decreased acid secretion and profound physio-morphological changes of the gastric mucosa. The purpose of the current study was to investigate the mechanisms triggering these pathophysiological changes. O. ostertagi infection resulted in a marked cellular hyperplasia, which can be explained by increased transcriptional levels of signaling molecules related to the homeostasis of gastric epithelial cells such as HES1, WNT5A, FGF10, HB-EGF, AREG, ADAM10 and ADAM17. Intriguingly, histological analysis indicated that the rapid rise in the gastric pH, observed following the emergence of adult worms, cannot be explained by a loss of parietal cells, as a decrease in the number of parietal cells was only observed following a long term infection of several weeks, but is likely to be caused by an inhibition of parietal cell activity. To investigate whether this inhibition is caused by a direct effect of the parasites, parietal cells were co-cultured with parasite Excretory/Secretory products (ESP) and subsequently analyzed for acid production. The results indicate that adult ESP inhibited acid secretion, whereas ESP from the L4 larval stages did not alter parietal cell function. In addition, our data show that the inhibition of parietal cell activity could be mediated by a marked upregulation of inflammatory factors, which are partly induced by adult ESP in abomasal epithelial cells. In conclusion, this study shows that the emergence of adult O. ostertagi worms is associated with marked cellular changes that can be partly triggered by the worm's Excretory/secretory antigens.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CIRCUMCINCTA, ACTIVATION, PROLIFERATION, ADULT, IN-VITRO, SHEEP, GASTRIC-CANCER, AMINOPYRINE ACCUMULATION, EXCRETORY/SECRETORY PRODUCTS, ACID-SECRETION
journal title
VETERINARY RESEARCH
Vet. Res.
volume
44
article number
121
pages
11 pages
Web of Science type
Article
Web of Science id
000328780800001
JCR category
VETERINARY SCIENCES
JCR impact factor
3.383 (2013)
JCR rank
1/132 (2013)
JCR quartile
1 (2013)
ISSN
0928-4249
DOI
10.1186/1297-9716-44-121
project
Concerted Research Actions of Ghent University (GOA)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
5639390
handle
http://hdl.handle.net/1854/LU-5639390
date created
2014-07-07 15:33:21
date last changed
2016-12-21 15:42:52
@article{5639390,
  abstract     = {Infections in cattle with the gastric nematode Ostertagia ostertagi are associated with decreased acid secretion and profound physio-morphological changes of the gastric mucosa. The purpose of the current study was to investigate the mechanisms triggering these pathophysiological changes. O. ostertagi infection resulted in a marked cellular hyperplasia, which can be explained by increased transcriptional levels of signaling molecules related to the homeostasis of gastric epithelial cells such as HES1, WNT5A, FGF10, HB-EGF, AREG, ADAM10 and ADAM17. Intriguingly, histological analysis indicated that the rapid rise in the gastric pH, observed following the emergence of adult worms, cannot be explained by a loss of parietal cells, as a decrease in the number of parietal cells was only observed following a long term infection of several weeks, but is likely to be caused by an inhibition of parietal cell activity. To investigate whether this inhibition is caused by a direct effect of the parasites, parietal cells were co-cultured with parasite Excretory/Secretory products (ESP) and subsequently analyzed for acid production. The results indicate that adult ESP inhibited acid secretion, whereas ESP from the L4 larval stages did not alter parietal cell function. In addition, our data show that the inhibition of parietal cell activity could be mediated by a marked upregulation of inflammatory factors, which are partly induced by adult ESP in abomasal epithelial cells. In conclusion, this study shows that the emergence of adult O. ostertagi worms is associated with marked cellular changes that can be partly triggered by the worm's Excretory/secretory antigens.},
  articleno    = {121},
  author       = {Mihi, Belgacem and Van Meulder, Frederik and Rinaldi, Manuela and Van Coppernolle, Stefanie and Chiers, Koen and Van Den Broeck, Wim and Goddeeris, Bruno and Vercruysse, Jozef and Claerebout, Edwin and Geldhof, Peter},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {CIRCUMCINCTA,ACTIVATION,PROLIFERATION,ADULT,IN-VITRO,SHEEP,GASTRIC-CANCER,AMINOPYRINE ACCUMULATION,EXCRETORY/SECRETORY PRODUCTS,ACID-SECRETION},
  language     = {eng},
  pages        = {11},
  title        = {Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection},
  url          = {http://dx.doi.org/10.1186/1297-9716-44-121},
  volume       = {44},
  year         = {2013},
}

Chicago
Mihi, Belgacem, Frederik Van Meulder, Manuela Rinaldi, Stefanie Van Coppernolle, Koen Chiers, Wim Van Den Broeck, Bruno Goddeeris, Jozef Vercruysse, Edwin Claerebout, and Peter Geldhof. 2013. “Analysis of Cell Hyperplasia and Parietal Cell Dysfunction Induced by Ostertagia Ostertagi Infection.” Veterinary Research 44.
APA
Mihi, B., Van Meulder, F., Rinaldi, M., Van Coppernolle, S., Chiers, K., Van Den Broeck, W., Goddeeris, B., et al. (2013). Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection. VETERINARY RESEARCH, 44.
Vancouver
1.
Mihi B, Van Meulder F, Rinaldi M, Van Coppernolle S, Chiers K, Van Den Broeck W, et al. Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection. VETERINARY RESEARCH. 2013;44.
MLA
Mihi, Belgacem, Frederik Van Meulder, Manuela Rinaldi, et al. “Analysis of Cell Hyperplasia and Parietal Cell Dysfunction Induced by Ostertagia Ostertagi Infection.” VETERINARY RESEARCH 44 (2013): n. pag. Print.