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Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection

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Concerted Research Actions of Ghent University (GOA)
Abstract
Infections in cattle with the gastric nematode Ostertagia ostertagi are associated with decreased acid secretion and profound physio-morphological changes of the gastric mucosa. The purpose of the current study was to investigate the mechanisms triggering these pathophysiological changes. O. ostertagi infection resulted in a marked cellular hyperplasia, which can be explained by increased transcriptional levels of signaling molecules related to the homeostasis of gastric epithelial cells such as HES1, WNT5A, FGF10, HB-EGF, AREG, ADAM10 and ADAM17. Intriguingly, histological analysis indicated that the rapid rise in the gastric pH, observed following the emergence of adult worms, cannot be explained by a loss of parietal cells, as a decrease in the number of parietal cells was only observed following a long term infection of several weeks, but is likely to be caused by an inhibition of parietal cell activity. To investigate whether this inhibition is caused by a direct effect of the parasites, parietal cells were co-cultured with parasite Excretory/Secretory products (ESP) and subsequently analyzed for acid production. The results indicate that adult ESP inhibited acid secretion, whereas ESP from the L4 larval stages did not alter parietal cell function. In addition, our data show that the inhibition of parietal cell activity could be mediated by a marked upregulation of inflammatory factors, which are partly induced by adult ESP in abomasal epithelial cells. In conclusion, this study shows that the emergence of adult O. ostertagi worms is associated with marked cellular changes that can be partly triggered by the worm's Excretory/secretory antigens.
Keywords
CIRCUMCINCTA, ACTIVATION, PROLIFERATION, ADULT, IN-VITRO, SHEEP, GASTRIC-CANCER, AMINOPYRINE ACCUMULATION, EXCRETORY/SECRETORY PRODUCTS, ACID-SECRETION

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Chicago
Mihi, Belgacem, Frederik Van Meulder, Manuela Rinaldi, Stefanie Van Coppernolle, Koen Chiers, Wim Van Den Broeck, Bruno Goddeeris, Jozef Vercruysse, Edwin Claerebout, and Peter Geldhof. 2013. “Analysis of Cell Hyperplasia and Parietal Cell Dysfunction Induced by Ostertagia Ostertagi Infection.” Veterinary Research 44.
APA
Mihi, B., Van Meulder, F., Rinaldi, M., Van Coppernolle, S., Chiers, K., Van Den Broeck, W., Goddeeris, B., et al. (2013). Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection. VETERINARY RESEARCH, 44.
Vancouver
1.
Mihi B, Van Meulder F, Rinaldi M, Van Coppernolle S, Chiers K, Van Den Broeck W, et al. Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection. VETERINARY RESEARCH. 2013;44.
MLA
Mihi, Belgacem, Frederik Van Meulder, Manuela Rinaldi, et al. “Analysis of Cell Hyperplasia and Parietal Cell Dysfunction Induced by Ostertagia Ostertagi Infection.” VETERINARY RESEARCH 44 (2013): n. pag. Print.
@article{5639390,
  abstract     = {Infections in cattle with the gastric nematode Ostertagia ostertagi are associated with decreased acid secretion and profound physio-morphological changes of the gastric mucosa. The purpose of the current study was to investigate the mechanisms triggering these pathophysiological changes. O. ostertagi infection resulted in a marked cellular hyperplasia, which can be explained by increased transcriptional levels of signaling molecules related to the homeostasis of gastric epithelial cells such as HES1, WNT5A, FGF10, HB-EGF, AREG, ADAM10 and ADAM17. Intriguingly, histological analysis indicated that the rapid rise in the gastric pH, observed following the emergence of adult worms, cannot be explained by a loss of parietal cells, as a decrease in the number of parietal cells was only observed following a long term infection of several weeks, but is likely to be caused by an inhibition of parietal cell activity. To investigate whether this inhibition is caused by a direct effect of the parasites, parietal cells were co-cultured with parasite Excretory/Secretory products (ESP) and subsequently analyzed for acid production. The results indicate that adult ESP inhibited acid secretion, whereas ESP from the L4 larval stages did not alter parietal cell function. In addition, our data show that the inhibition of parietal cell activity could be mediated by a marked upregulation of inflammatory factors, which are partly induced by adult ESP in abomasal epithelial cells. In conclusion, this study shows that the emergence of adult O. ostertagi worms is associated with marked cellular changes that can be partly triggered by the worm's Excretory/secretory antigens.},
  articleno    = {121},
  author       = {Mihi, Belgacem and Van Meulder, Frederik and Rinaldi, Manuela and Van Coppernolle, Stefanie and Chiers, Koen and Van Den Broeck, Wim and Goddeeris, Bruno and Vercruysse, Jozef and Claerebout, Edwin and Geldhof, Peter},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {CIRCUMCINCTA,ACTIVATION,PROLIFERATION,ADULT,IN-VITRO,SHEEP,GASTRIC-CANCER,AMINOPYRINE ACCUMULATION,EXCRETORY/SECRETORY PRODUCTS,ACID-SECRETION},
  language     = {eng},
  pages        = {11},
  title        = {Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection},
  url          = {http://dx.doi.org/10.1186/1297-9716-44-121},
  volume       = {44},
  year         = {2013},
}

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