Advanced search

Simultaneous separation of eight beta-adrenergic drugs using titanium dioxide nanoparticles as additive in capillary electrophoresis

(2008) ELECTROPHORESIS. 29(11). p.2321-2329
Author
Organization
Abstract
The analysis is described for separating seven beta-adrenergic blocking agents (atenolol, celiprolol, clorprenaline, fenoterol, metoprolol, propranolol, terbutaline) and clenbuterol (sympathomimetic beta-2 receptor stimulating agonist, decongestant and bronchodilator, illicit anabolic used in athletics) by CE with UV detection. In order to simultaneously separate all analytes, Tris-H3PO4 solution was applied containing titanium dioxide nanoparticles (TiO2 NPs) as BGEs. The effects of important factors, such as concentration of TiO2. NPs, optimum pH, run buffer concentration, and separation voltage, were investigated so as to achieve best CE separation. The eight analytes could be well separated applying a separation voltage of 15 kV in 75 mM Tris-H3PO4 buffer at a pH of 2.40, containing 6.0 x 10(-6) g/mL TiO2 NPs. Under these optimal conditions, the RSDs for peak areas and for migration times were less than 2.7 and 2.3%, respectively. The detection limits were 0.1 mu g/mL for celiprolol, 0.1 mu g/mL for propranolol, 0.2 mu g/mL for fenoterol, 1.0 mu g/mL for atenolol, 1.0 mu g/mL, for clenbuterol, 1.0 mu g/mL for clorprenaline, 1.0 mu g/mL for metoprolol, and 1.0 mu g/mL for terbutaline. The proposed method was successfully applied for the rapid CE determination of the frequently applied antihypertensive P-blocking compounds atenolol, metoprolol, terbutaline, and propranolol in pharmaceutical tablets.
Keywords
SOLUTION INTERFACE, BLOCKERS, STATIONARY-PHASE, ZONE-ELECTROPHORESIS, LIQUID-CHROMATOGRAPHY, beta-adrenergic antagonists, beta-adrenergic agonist, capillary electrophoresis, titanium dioxide nanoparticles, IONIZATION-MASS-SPECTROMETRY, PART I, CARBON NANOTUBES, ELECTROKINETIC CHROMATOGRAPHY, ELECTROCHROMATOGRAPHIC SEPARATION

Citation

Please use this url to cite or link to this publication:

Chicago
Zhou, S, Y Wang, Thomas De Beer, Willy Baeyens, GT Fei, M Dilinuer, and J Ouyang. 2008. “Simultaneous Separation of Eight Beta-adrenergic Drugs Using Titanium Dioxide Nanoparticles as Additive in Capillary Electrophoresis.” Electrophoresis 29 (11): 2321–2329.
APA
Zhou, S., Wang, Y., De Beer, T., Baeyens, W., Fei, G., Dilinuer, M., & Ouyang, J. (2008). Simultaneous separation of eight beta-adrenergic drugs using titanium dioxide nanoparticles as additive in capillary electrophoresis. ELECTROPHORESIS, 29(11), 2321–2329.
Vancouver
1.
Zhou S, Wang Y, De Beer T, Baeyens W, Fei G, Dilinuer M, et al. Simultaneous separation of eight beta-adrenergic drugs using titanium dioxide nanoparticles as additive in capillary electrophoresis. ELECTROPHORESIS. PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY: WILEY-V C H VERLAG GMBH; 2008;29(11):2321–9.
MLA
Zhou, S, Y Wang, Thomas De Beer, et al. “Simultaneous Separation of Eight Beta-adrenergic Drugs Using Titanium Dioxide Nanoparticles as Additive in Capillary Electrophoresis.” ELECTROPHORESIS 29.11 (2008): 2321–2329. Print.
@article{539317,
  abstract     = {The analysis is described for separating seven beta-adrenergic blocking agents (atenolol, celiprolol, clorprenaline, fenoterol, metoprolol, propranolol, terbutaline) and clenbuterol (sympathomimetic beta-2 receptor stimulating agonist, decongestant and bronchodilator, illicit anabolic used in athletics) by CE with UV detection. In order to simultaneously separate all analytes, Tris-H3PO4 solution was applied containing titanium dioxide nanoparticles (TiO2 NPs) as BGEs. The effects of important factors, such as concentration of TiO2. NPs, optimum pH, run buffer concentration, and separation voltage, were investigated so as to achieve best CE separation. The eight analytes could be well separated applying a separation voltage of 15 kV in 75 mM Tris-H3PO4 buffer at a pH of 2.40, containing 6.0 x 10(-6) g/mL TiO2 NPs. Under these optimal conditions, the RSDs for peak areas and for migration times were less than 2.7 and 2.3\%, respectively. The detection limits were 0.1 mu g/mL for celiprolol, 0.1 mu g/mL for propranolol, 0.2 mu g/mL for fenoterol, 1.0 mu g/mL for atenolol, 1.0 mu g/mL, for clenbuterol, 1.0 mu g/mL for clorprenaline, 1.0 mu g/mL for metoprolol, and 1.0 mu g/mL for terbutaline. The proposed method was successfully applied for the rapid CE determination of the frequently applied antihypertensive P-blocking compounds atenolol, metoprolol, terbutaline, and propranolol in pharmaceutical tablets.},
  author       = {Zhou, S and Wang, Y and De Beer, Thomas and Baeyens, Willy and Fei, GT and Dilinuer, M and Ouyang, J},
  issn         = {0173-0835},
  journal      = {ELECTROPHORESIS},
  keyword      = {SOLUTION INTERFACE,BLOCKERS,STATIONARY-PHASE,ZONE-ELECTROPHORESIS,LIQUID-CHROMATOGRAPHY,beta-adrenergic antagonists,beta-adrenergic agonist,capillary electrophoresis,titanium dioxide nanoparticles,IONIZATION-MASS-SPECTROMETRY,PART I,CARBON NANOTUBES,ELECTROKINETIC CHROMATOGRAPHY,ELECTROCHROMATOGRAPHIC SEPARATION},
  language     = {eng},
  number       = {11},
  pages        = {2321--2329},
  publisher    = {WILEY-V C H VERLAG GMBH},
  title        = {Simultaneous separation of eight beta-adrenergic drugs using titanium dioxide nanoparticles as additive in capillary electrophoresis},
  url          = {http://dx.doi.org/10.1002/elps.200700589},
  volume       = {29},
  year         = {2008},
}

Altmetric
View in Altmetric
Web of Science
Times cited: