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Separation of purine and pyrimidine bases by ion chromatography with direct conductivity detection

L Liu, J Ouyang and Willy Baeyens (2008) JOURNAL OF CHROMATOGRAPHY A. 1193(1-2). p.104-108
abstract
A simple, rapid and accurate method for the simultaneous determination of four purine and pyrimidine bases (cytosine, 5-methylcytosine, adenine and N6-methyladenine) has been developed. The quantitative determination of these bases was accomplished by ion chromatography (IC) with direct conductivity detection (CD) based on their ionization in acidic medium without chemical suppression. The recovery of cytosine, 5-methylcytosine, and adenine in calf thymus DNA was more than 98% (n = 3) and the relative standard deviation (RSD, n = 5) less than 2.4%. In a single chromatographic run, the four bases could be separated and determined in less than 10 min. The detection limits were found to be 0.05 mu g/mL for cytosine, 0.08 mu g/mL for 5-methylcytosine, 0.07 mu g/mL for adenine, and 0.07 mu g/mL for N6-methyladenine. Linear ranges were 0.2-95.1 mu g/mL for cytosine (r(2) = 0.9996),0.3-196.6 mu g/mL for 5-methylcytosine (r(2) = 0.9994), 0.3-105.5 mu g/mL for adenine (r(2) = 0.9998), and 0.3-159.1 mu g/mL for N6-methyladenine (r(2) = 0.9999). With the proposed method, purine and pyrimidine bases could be successfully detected in calf thymus DNA. We also determined these bases in calf thymus DNA using RP-HPLC. Compared to RP-HPLC, the IC method offers advantages such as high selectivity and simple mobile phase.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
5-methylcytosine, cytosine, adenine, N6-methyladenine, ion chromatography, conductivity detection, PERFORMANCE LIQUID-CHROMATOGRAPHY, DNA METHYLATION, CANCER, NUCLEOSIDES, CORDYCEPS, SILICA
journal title
JOURNAL OF CHROMATOGRAPHY A
J. Chromatogr. A
volume
1193
issue
1-2
pages
104 - 108
publisher
Elsevier Science
place of publication
PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
Web of Science type
Article
Web of Science id
000256558600014
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.756 (2008)
JCR rank
6/70 (2008)
JCR quartile
1 (2008)
ISSN
0021-9673
DOI
10.1016/j.chroma.2008.04.011
language
English
UGent publication?
yes
classification
A1
copyright statement
I don't know the status of the copyright for this publication
id
539305
handle
http://hdl.handle.net/1854/LU-539305
date created
2009-04-07 15:22:32
date last changed
2017-01-02 09:56:00
@article{539305,
  abstract     = {A simple, rapid and accurate method for the simultaneous determination of four purine and pyrimidine bases (cytosine, 5-methylcytosine, adenine and N6-methyladenine) has been developed. The quantitative determination of these bases was accomplished by ion chromatography (IC) with direct conductivity detection (CD) based on their ionization in acidic medium without chemical suppression. The recovery of cytosine, 5-methylcytosine, and adenine in calf thymus DNA was more than 98\% (n = 3) and the relative standard deviation (RSD, n = 5) less than 2.4\%. In a single chromatographic run, the four bases could be separated and determined in less than 10 min. The detection limits were found to be 0.05 mu g/mL for cytosine, 0.08 mu g/mL for 5-methylcytosine, 0.07 mu g/mL for adenine, and 0.07 mu g/mL for N6-methyladenine. Linear ranges were 0.2-95.1 mu g/mL for cytosine (r(2) = 0.9996),0.3-196.6 mu g/mL for 5-methylcytosine (r(2) = 0.9994), 0.3-105.5 mu g/mL for adenine (r(2) = 0.9998), and 0.3-159.1 mu g/mL for N6-methyladenine (r(2) = 0.9999). With the proposed method, purine and pyrimidine bases could be successfully detected in calf thymus DNA. We also determined these bases in calf thymus DNA using RP-HPLC. Compared to RP-HPLC, the IC method offers advantages such as high selectivity and simple mobile phase.},
  author       = {Liu, L and Ouyang, J and Baeyens, Willy},
  issn         = {0021-9673},
  journal      = {JOURNAL OF CHROMATOGRAPHY A},
  keyword      = {5-methylcytosine,cytosine,adenine,N6-methyladenine,ion chromatography,conductivity detection,PERFORMANCE LIQUID-CHROMATOGRAPHY,DNA METHYLATION,CANCER,NUCLEOSIDES,CORDYCEPS,SILICA},
  language     = {eng},
  number       = {1-2},
  pages        = {104--108},
  publisher    = {Elsevier Science},
  title        = {Separation of purine and pyrimidine bases by ion chromatography with direct conductivity detection},
  url          = {http://dx.doi.org/10.1016/j.chroma.2008.04.011},
  volume       = {1193},
  year         = {2008},
}

Chicago
Liu, L, J Ouyang, and Willy Baeyens. 2008. “Separation of Purine and Pyrimidine Bases by Ion Chromatography with Direct Conductivity Detection.” Journal of Chromatography A 1193 (1-2): 104–108.
APA
Liu, L, Ouyang, J., & Baeyens, W. (2008). Separation of purine and pyrimidine bases by ion chromatography with direct conductivity detection. JOURNAL OF CHROMATOGRAPHY A, 1193(1-2), 104–108.
Vancouver
1.
Liu L, Ouyang J, Baeyens W. Separation of purine and pyrimidine bases by ion chromatography with direct conductivity detection. JOURNAL OF CHROMATOGRAPHY A. PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS: Elsevier Science; 2008;1193(1-2):104–8.
MLA
Liu, L, J Ouyang, and Willy Baeyens. “Separation of Purine and Pyrimidine Bases by Ion Chromatography with Direct Conductivity Detection.” JOURNAL OF CHROMATOGRAPHY A 1193.1-2 (2008): 104–108. Print.