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A novel probe Au(III) for chemiluminescent image detection of protein blots on nitrocellulose membranes

Jia Liu, Xia Liu, Willy Baeyens UGent, Joris Delanghe UGent and Jin Ouyang (2008) JOURNAL OF PROTEOME RESEARCH. 7(5). p.1884-1890
abstract
A new method, based on the chloroauric acid-enhanced luminol chemiluminescence, is established for the chemiluminescent imaging detection of protein blots on nitrocellulose membranes. After transferring to the nitrocellulose (NC) membranes, various proteins in human serum can be easily detected using this method. Simplicity and wide applicability are achieved, without the need of expensive antibodies or tedious immunoassay procedures. Furthermore, neither noxious materials nor radioactive pollution is produced. The successful detection of proteins is due to the binding of Au(III) to the protein blots and the chemiluminescent character of the enhanced luminol signal. As a novel chemiluminescent detection method, it offers significant biological analytical potentials in biochemistry and in molecular biology.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
chloroauric acid, POLYACRYLAMIDE-GEL ELECTROPHORESIS, chemiluminescent imaging, polyacrylamide gel electrophoresis, human serum proteins, Western blotting, COMPLEX, GOLD, PEPTIDES
journal title
JOURNAL OF PROTEOME RESEARCH
J. Proteome Res.
volume
7
issue
5
pages
1884 - 1890
Web of Science type
Article
Web of Science id
000255520200009
JCR category
BIOCHEMICAL RESEARCH METHODS
JCR impact factor
5.684 (2008)
JCR rank
8/65 (2008)
JCR quartile
1 (2008)
ISSN
1535-3893
DOI
10.1021/pr700616u
language
English
UGent publication?
yes
classification
A1
id
539237
handle
http://hdl.handle.net/1854/LU-539237
date created
2009-04-07 14:10:40
date last changed
2010-04-28 10:20:31
@article{539237,
  abstract     = {A new method, based on the chloroauric acid-enhanced luminol chemiluminescence, is established for the chemiluminescent imaging detection of protein blots on nitrocellulose membranes. After transferring to the nitrocellulose (NC) membranes, various proteins in human serum can be easily detected using this method. Simplicity and wide applicability are achieved, without the need of expensive antibodies or tedious immunoassay procedures. Furthermore, neither noxious materials nor radioactive pollution is produced. The successful detection of proteins is due to the binding of Au(III) to the protein blots and the chemiluminescent character of the enhanced luminol signal. As a novel chemiluminescent detection method, it offers significant biological analytical potentials in biochemistry and in molecular biology.},
  author       = {Liu, Jia and Liu, Xia and Baeyens, Willy and Delanghe, Joris and Ouyang, Jin},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  keyword      = {chloroauric acid,POLYACRYLAMIDE-GEL ELECTROPHORESIS,chemiluminescent imaging,polyacrylamide gel electrophoresis,human serum proteins,Western blotting,COMPLEX,GOLD,PEPTIDES},
  language     = {eng},
  number       = {5},
  pages        = {1884--1890},
  title        = {A novel probe Au(III) for chemiluminescent image detection of protein blots on nitrocellulose membranes},
  url          = {http://dx.doi.org/10.1021/pr700616u},
  volume       = {7},
  year         = {2008},
}

Chicago
Liu, Jia, Xia Liu, Willy Baeyens, Joris Delanghe, and Jin Ouyang. 2008. “A Novel Probe Au(III) for Chemiluminescent Image Detection of Protein Blots on Nitrocellulose Membranes.” Journal of Proteome Research 7 (5): 1884–1890.
APA
Liu, Jia, Liu, X., Baeyens, W., Delanghe, J., & Ouyang, J. (2008). A novel probe Au(III) for chemiluminescent image detection of protein blots on nitrocellulose membranes. JOURNAL OF PROTEOME RESEARCH, 7(5), 1884–1890.
Vancouver
1.
Liu J, Liu X, Baeyens W, Delanghe J, Ouyang J. A novel probe Au(III) for chemiluminescent image detection of protein blots on nitrocellulose membranes. JOURNAL OF PROTEOME RESEARCH. 2008;7(5):1884–90.
MLA
Liu, Jia, Xia Liu, Willy Baeyens, et al. “A Novel Probe Au(III) for Chemiluminescent Image Detection of Protein Blots on Nitrocellulose Membranes.” JOURNAL OF PROTEOME RESEARCH 7.5 (2008): 1884–1890. Print.