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A novel probe Au(III) for chemiluminescent image detection of protein blots on nitrocellulose membranes

(2008) JOURNAL OF PROTEOME RESEARCH. 7(5). p.1884-1890
Author
Organization
Abstract
A new method, based on the chloroauric acid-enhanced luminol chemiluminescence, is established for the chemiluminescent imaging detection of protein blots on nitrocellulose membranes. After transferring to the nitrocellulose (NC) membranes, various proteins in human serum can be easily detected using this method. Simplicity and wide applicability are achieved, without the need of expensive antibodies or tedious immunoassay procedures. Furthermore, neither noxious materials nor radioactive pollution is produced. The successful detection of proteins is due to the binding of Au(III) to the protein blots and the chemiluminescent character of the enhanced luminol signal. As a novel chemiluminescent detection method, it offers significant biological analytical potentials in biochemistry and in molecular biology.
Keywords
chloroauric acid, POLYACRYLAMIDE-GEL ELECTROPHORESIS, chemiluminescent imaging, polyacrylamide gel electrophoresis, human serum proteins, Western blotting, COMPLEX, GOLD, PEPTIDES

Citation

Please use this url to cite or link to this publication:

MLA
Liu, Jia, Xia Liu, Willy Baeyens, et al. “A Novel Probe Au(III) for Chemiluminescent Image Detection of Protein Blots on Nitrocellulose Membranes.” JOURNAL OF PROTEOME RESEARCH 7.5 (2008): 1884–1890. Print.
APA
Liu, Jia, Liu, X., Baeyens, W., Delanghe, J., & Ouyang, J. (2008). A novel probe Au(III) for chemiluminescent image detection of protein blots on nitrocellulose membranes. JOURNAL OF PROTEOME RESEARCH, 7(5), 1884–1890.
Chicago author-date
Liu, Jia, Xia Liu, Willy Baeyens, Joris Delanghe, and Jin Ouyang. 2008. “A Novel Probe Au(III) for Chemiluminescent Image Detection of Protein Blots on Nitrocellulose Membranes.” Journal of Proteome Research 7 (5): 1884–1890.
Chicago author-date (all authors)
Liu, Jia, Xia Liu, Willy Baeyens, Joris Delanghe, and Jin Ouyang. 2008. “A Novel Probe Au(III) for Chemiluminescent Image Detection of Protein Blots on Nitrocellulose Membranes.” Journal of Proteome Research 7 (5): 1884–1890.
Vancouver
1.
Liu J, Liu X, Baeyens W, Delanghe J, Ouyang J. A novel probe Au(III) for chemiluminescent image detection of protein blots on nitrocellulose membranes. JOURNAL OF PROTEOME RESEARCH. 2008;7(5):1884–90.
IEEE
[1]
J. Liu, X. Liu, W. Baeyens, J. Delanghe, and J. Ouyang, “A novel probe Au(III) for chemiluminescent image detection of protein blots on nitrocellulose membranes,” JOURNAL OF PROTEOME RESEARCH, vol. 7, no. 5, pp. 1884–1890, 2008.
@article{539237,
  abstract     = {A new method, based on the chloroauric acid-enhanced luminol chemiluminescence, is established for the chemiluminescent imaging detection of protein blots on nitrocellulose membranes. After transferring to the nitrocellulose (NC) membranes, various proteins in human serum can be easily detected using this method. Simplicity and wide applicability are achieved, without the need of expensive antibodies or tedious immunoassay procedures. Furthermore, neither noxious materials nor radioactive pollution is produced. The successful detection of proteins is due to the binding of Au(III) to the protein blots and the chemiluminescent character of the enhanced luminol signal. As a novel chemiluminescent detection method, it offers significant biological analytical potentials in biochemistry and in molecular biology.},
  author       = {Liu, Jia and Liu, Xia and Baeyens, Willy and Delanghe, Joris and Ouyang, Jin},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  keywords     = {chloroauric acid,POLYACRYLAMIDE-GEL ELECTROPHORESIS,chemiluminescent imaging,polyacrylamide gel electrophoresis,human serum proteins,Western blotting,COMPLEX,GOLD,PEPTIDES},
  language     = {eng},
  number       = {5},
  pages        = {1884--1890},
  title        = {A novel probe Au(III) for chemiluminescent image detection of protein blots on nitrocellulose membranes},
  url          = {http://dx.doi.org/10.1021/pr700616u},
  volume       = {7},
  year         = {2008},
}

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