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AtCDKA;1 silencing in Arabidopsis thaliana reduces reproduction of sedentary plant-parasitic nematodes

Elke Van de Cappelle, Eva Plovie, Tina Kyndt UGent, Wim Grunewald UGent, Bernard Cannoot UGent and Godelieve Gheysen UGent (2008) PLANT BIOTECHNOLOGY JOURNAL. 6(8). p.749-757
abstract
The activity of the Arabidopsis thaliana cyclin-dependent kinase AtCDKA;1 is important throughout G(1)/S and G(2)/M transitions and guarantees the progression of the cell cycle. Inhibitor studies have shown that activation of the cell cycle is important for the development of nematode feeding sites. The aim of this study was to silence the expression of the AtCDKA;1 gene in nematode feeding sites to interfere with their development. Therefore, sense and antisense constructs were made for the AtCDKA;1 gene and fused to a nematode-inducible promoter which was activated in nematode feeding sites at an earlier time point than AtCDKA;1. Two transgenic A. thaliana lines (S266 and S306) containing inverted repeats of the AtCDKA;1 gene and with reduced AtCDKA;1 expression in seedlings and galls were analysed in more detail, When the lines were infected with the root-knot nematode Meloidogyne incognita, significantly fewer galls and egg masses developed on the roots of the transgenic than wild-type plants. infection of the AtCDKA;1-silenced lines with Heterodera schachtii resulted in significantly fewer cysts compared with controls. The S266 and S306 lines showed no phenotypic aberrations in root morphology, and analysis at different time points after infection demonstrated that the number of penetrating nematodes was the same, but fewer nematodes developed to maturity in the silenced lines. In conclusion, our results demonstrate that silencing of CDKA;1 can be used as a strategy to produce transgenic plants less susceptible to plant-parasitic nematodes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
silencing, resistance, VIRUS 35S PROMOTER, GENE-EXPRESSION, MEDIATED TRANSFORMATION, FEEDING STRUCTURES, CELL-DIVISION, HOST, SEQUENCES, MUTANTS, KINASE, Meloidogyne incognita, Heterodera schachtii, feeding site, cell cycle, inverted repeat, ROOTS
journal title
PLANT BIOTECHNOLOGY JOURNAL
Plant Biotechnol. J.
volume
6
issue
8
pages
749 - 757
Web of Science type
Article
Web of Science id
000259641700001
JCR category
PLANT SCIENCES
JCR impact factor
4.419 (2008)
JCR rank
15/155 (2008)
JCR quartile
1 (2008)
ISSN
1467-7644
DOI
10.1111/j.1467-7652.2008.00355.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
538887
handle
http://hdl.handle.net/1854/LU-538887
date created
2009-04-06 18:16:33
date last changed
2012-12-06 16:54:31
@article{538887,
  abstract     = {The activity of the Arabidopsis thaliana cyclin-dependent kinase AtCDKA;1 is important throughout G(1)/S and G(2)/M transitions and guarantees the progression of the cell cycle. Inhibitor studies have shown that activation of the cell cycle is important for the development of nematode feeding sites. The aim of this study was to silence the expression of the AtCDKA;1 gene in nematode feeding sites to interfere with their development. Therefore, sense and antisense constructs were made for the AtCDKA;1 gene and fused to a nematode-inducible promoter which was activated in nematode feeding sites at an earlier time point than AtCDKA;1. Two transgenic A. thaliana lines (S266 and S306) containing inverted repeats of the AtCDKA;1 gene and with reduced AtCDKA;1 expression in seedlings and galls were analysed in more detail, When the lines were infected with the root-knot nematode Meloidogyne incognita, significantly fewer galls and egg masses developed on the roots of the transgenic than wild-type plants. infection of the AtCDKA;1-silenced lines with Heterodera schachtii resulted in significantly fewer cysts compared with controls. The S266 and S306 lines showed no phenotypic aberrations in root morphology, and analysis at different time points after infection demonstrated that the number of penetrating nematodes was the same, but fewer nematodes developed to maturity in the silenced lines. In conclusion, our results demonstrate that silencing of CDKA;1 can be used as a strategy to produce transgenic plants less susceptible to plant-parasitic nematodes.},
  author       = {Van de Cappelle, Elke and Plovie, Eva and Kyndt, Tina and Grunewald, Wim and Cannoot, Bernard and Gheysen, Godelieve},
  issn         = {1467-7644},
  journal      = {PLANT BIOTECHNOLOGY JOURNAL},
  keyword      = {silencing,resistance,VIRUS 35S PROMOTER,GENE-EXPRESSION,MEDIATED TRANSFORMATION,FEEDING STRUCTURES,CELL-DIVISION,HOST,SEQUENCES,MUTANTS,KINASE,Meloidogyne incognita,Heterodera schachtii,feeding site,cell cycle,inverted repeat,ROOTS},
  language     = {eng},
  number       = {8},
  pages        = {749--757},
  title        = {AtCDKA;1 silencing in Arabidopsis thaliana reduces reproduction of sedentary plant-parasitic nematodes},
  url          = {http://dx.doi.org/10.1111/j.1467-7652.2008.00355.x},
  volume       = {6},
  year         = {2008},
}

Chicago
Van de Cappelle, Elke, Eva Plovie, Tina Kyndt, Wim Grunewald, Bernard Cannoot, and Godelieve Gheysen. 2008. “AtCDKA;1 Silencing in Arabidopsis Thaliana Reduces Reproduction of Sedentary Plant-parasitic Nematodes.” Plant Biotechnology Journal 6 (8): 749–757.
APA
Van de Cappelle, E., Plovie, E., Kyndt, T., Grunewald, W., Cannoot, B., & Gheysen, G. (2008). AtCDKA;1 silencing in Arabidopsis thaliana reduces reproduction of sedentary plant-parasitic nematodes. PLANT BIOTECHNOLOGY JOURNAL, 6(8), 749–757.
Vancouver
1.
Van de Cappelle E, Plovie E, Kyndt T, Grunewald W, Cannoot B, Gheysen G. AtCDKA;1 silencing in Arabidopsis thaliana reduces reproduction of sedentary plant-parasitic nematodes. PLANT BIOTECHNOLOGY JOURNAL. 2008;6(8):749–57.
MLA
Van de Cappelle, Elke, Eva Plovie, Tina Kyndt, et al. “AtCDKA;1 Silencing in Arabidopsis Thaliana Reduces Reproduction of Sedentary Plant-parasitic Nematodes.” PLANT BIOTECHNOLOGY JOURNAL 6.8 (2008): 749–757. Print.