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Analysis of proteins in Chlamydia trachomatis L2 outer membrane complex, COMC

Svend Birkelund, Marie Morgan-Fisher, Evy Timmerman UGent, Kris Gevaert UGent, Allan C. Shaw and Gunna Christiansen (2009) FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY. 55(2). p.187-195
abstract
The protein composition and N-terminal sequences of proteins in the outer membrane of Chlamydia trachomatis L2 were analysed following isolation of N-terminal peptides using combined fractional diagonal chromatography and identification by liquid chromatography tandem MS. Acetylation of primary amino groups of in vivo generated proteolytic cleavage sites facilitated identification of such sites in known outer membrane proteins (MOMPs). Our results further support a proposed prediction of the topology of the MOMPs. Furthermore, a previously unknown MOMP, CTL0626 (Ct372), was assigned as an MOMP with a carbohydrate-selective porin (OprB) family motif, and the presence of CTL0626 was confirmed using antibodies raised against the protein.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Chlamydia outer membrane complex, combined fractional diagonal chromatography, carbohydrate-selective porin, PSEUDOMONAS-AERUGINOSA, SEQUENCE-ANALYSIS, SEROVAR L2, IDENTIFICATION, DATABASES, PORIN, EPITOPE, PEPTIDES, OPRB, SURFACE, Chlamydia trachomatis L2
journal title
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
FEMS Immunol. Med. Microbiol.
volume
55
issue
2
pages
187 - 195
publisher
WILEY-BLACKWELL PUBLISHING, INC
place of publication
MALDEN 02148, MA USA
Web of Science type
Article
Web of Science id
000263133600010
JCR category
MICROBIOLOGY
JCR impact factor
2.335 (2009)
JCR rank
50/94 (2009)
JCR quartile
3 (2009)
ISSN
0928-8244
DOI
10.1111/j.1574-695X.2009.00522.x
language
English
UGent publication?
yes
classification
A1
id
537618
handle
http://hdl.handle.net/1854/LU-537618
date created
2009-04-03 11:30:39
date last changed
2013-01-30 09:41:40
@article{537618,
  abstract     = {The protein composition and N-terminal sequences of proteins in the outer membrane of Chlamydia trachomatis L2 were analysed following isolation of N-terminal peptides using combined fractional diagonal chromatography and identification by liquid chromatography tandem MS. Acetylation of primary amino groups of in vivo generated proteolytic cleavage sites facilitated identification of such sites in known outer membrane proteins (MOMPs). Our results further support a proposed prediction of the topology of the MOMPs. Furthermore, a previously unknown MOMP, CTL0626 (Ct372), was assigned as an MOMP with a carbohydrate-selective porin (OprB) family motif, and the presence of CTL0626 was confirmed using antibodies raised against the protein.},
  author       = {Birkelund, Svend and Morgan-Fisher, Marie and Timmerman, Evy and Gevaert, Kris and Shaw, Allan C. and Christiansen, Gunna},
  issn         = {0928-8244},
  journal      = {FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY},
  keyword      = {Chlamydia outer membrane complex,combined fractional diagonal chromatography,carbohydrate-selective porin,PSEUDOMONAS-AERUGINOSA,SEQUENCE-ANALYSIS,SEROVAR L2,IDENTIFICATION,DATABASES,PORIN,EPITOPE,PEPTIDES,OPRB,SURFACE,Chlamydia trachomatis L2},
  language     = {eng},
  number       = {2},
  pages        = {187--195},
  publisher    = {WILEY-BLACKWELL PUBLISHING, INC},
  title        = {Analysis of proteins in Chlamydia trachomatis L2 outer membrane complex, COMC},
  url          = {http://dx.doi.org/10.1111/j.1574-695X.2009.00522.x},
  volume       = {55},
  year         = {2009},
}

Chicago
Birkelund, Svend, Marie Morgan-Fisher, Evy Timmerman, Kris Gevaert, Allan C. Shaw, and Gunna Christiansen. 2009. “Analysis of Proteins in Chlamydia Trachomatis L2 Outer Membrane Complex, COMC.” Fems Immunology and Medical Microbiology 55 (2): 187–195.
APA
Birkelund, S., Morgan-Fisher, M., Timmerman, E., Gevaert, K., Shaw, A. C., & Christiansen, G. (2009). Analysis of proteins in Chlamydia trachomatis L2 outer membrane complex, COMC. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 55(2), 187–195.
Vancouver
1.
Birkelund S, Morgan-Fisher M, Timmerman E, Gevaert K, Shaw AC, Christiansen G. Analysis of proteins in Chlamydia trachomatis L2 outer membrane complex, COMC. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY. MALDEN 02148, MA USA: WILEY-BLACKWELL PUBLISHING, INC; 2009;55(2):187–95.
MLA
Birkelund, Svend, Marie Morgan-Fisher, Evy Timmerman, et al. “Analysis of Proteins in Chlamydia Trachomatis L2 Outer Membrane Complex, COMC.” FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY 55.2 (2009): 187–195. Print.