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Analysis of proteins in Chlamydia trachomatis L2 outer membrane complex, COMC

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Abstract
The protein composition and N-terminal sequences of proteins in the outer membrane of Chlamydia trachomatis L2 were analysed following isolation of N-terminal peptides using combined fractional diagonal chromatography and identification by liquid chromatography tandem MS. Acetylation of primary amino groups of in vivo generated proteolytic cleavage sites facilitated identification of such sites in known outer membrane proteins (MOMPs). Our results further support a proposed prediction of the topology of the MOMPs. Furthermore, a previously unknown MOMP, CTL0626 (Ct372), was assigned as an MOMP with a carbohydrate-selective porin (OprB) family motif, and the presence of CTL0626 was confirmed using antibodies raised against the protein.
Keywords
Chlamydia outer membrane complex, combined fractional diagonal chromatography, carbohydrate-selective porin, PSEUDOMONAS-AERUGINOSA, SEQUENCE-ANALYSIS, SEROVAR L2, IDENTIFICATION, DATABASES, PORIN, EPITOPE, PEPTIDES, OPRB, SURFACE, Chlamydia trachomatis L2

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MLA
Birkelund, Svend, Marie Morgan-Fisher, Evy Timmerman, et al. “Analysis of Proteins in Chlamydia Trachomatis L2 Outer Membrane Complex, COMC.” FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY 55.2 (2009): 187–195. Print.
APA
Birkelund, S., Morgan-Fisher, M., Timmerman, E., Gevaert, K., Shaw, A. C., & Christiansen, G. (2009). Analysis of proteins in Chlamydia trachomatis L2 outer membrane complex, COMC. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 55(2), 187–195.
Chicago author-date
Birkelund, Svend, Marie Morgan-Fisher, Evy Timmerman, Kris Gevaert, Allan C. Shaw, and Gunna Christiansen. 2009. “Analysis of Proteins in Chlamydia Trachomatis L2 Outer Membrane Complex, COMC.” Fems Immunology and Medical Microbiology 55 (2): 187–195.
Chicago author-date (all authors)
Birkelund, Svend, Marie Morgan-Fisher, Evy Timmerman, Kris Gevaert, Allan C. Shaw, and Gunna Christiansen. 2009. “Analysis of Proteins in Chlamydia Trachomatis L2 Outer Membrane Complex, COMC.” Fems Immunology and Medical Microbiology 55 (2): 187–195.
Vancouver
1.
Birkelund S, Morgan-Fisher M, Timmerman E, Gevaert K, Shaw AC, Christiansen G. Analysis of proteins in Chlamydia trachomatis L2 outer membrane complex, COMC. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY. MALDEN 02148, MA USA: WILEY-BLACKWELL PUBLISHING, INC; 2009;55(2):187–95.
IEEE
[1]
S. Birkelund, M. Morgan-Fisher, E. Timmerman, K. Gevaert, A. C. Shaw, and G. Christiansen, “Analysis of proteins in Chlamydia trachomatis L2 outer membrane complex, COMC,” FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, vol. 55, no. 2, pp. 187–195, 2009.
@article{537618,
  abstract     = {The protein composition and N-terminal sequences of proteins in the outer membrane of Chlamydia trachomatis L2 were analysed following isolation of N-terminal peptides using combined fractional diagonal chromatography and identification by liquid chromatography tandem MS. Acetylation of primary amino groups of in vivo generated proteolytic cleavage sites facilitated identification of such sites in known outer membrane proteins (MOMPs). Our results further support a proposed prediction of the topology of the MOMPs. Furthermore, a previously unknown MOMP, CTL0626 (Ct372), was assigned as an MOMP with a carbohydrate-selective porin (OprB) family motif, and the presence of CTL0626 was confirmed using antibodies raised against the protein.},
  author       = {Birkelund, Svend and Morgan-Fisher, Marie and Timmerman, Evy and Gevaert, Kris and Shaw, Allan C. and Christiansen, Gunna},
  issn         = {0928-8244},
  journal      = {FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY},
  keywords     = {Chlamydia outer membrane complex,combined fractional diagonal chromatography,carbohydrate-selective porin,PSEUDOMONAS-AERUGINOSA,SEQUENCE-ANALYSIS,SEROVAR L2,IDENTIFICATION,DATABASES,PORIN,EPITOPE,PEPTIDES,OPRB,SURFACE,Chlamydia trachomatis L2},
  language     = {eng},
  number       = {2},
  pages        = {187--195},
  publisher    = {WILEY-BLACKWELL PUBLISHING, INC},
  title        = {Analysis of proteins in Chlamydia trachomatis L2 outer membrane complex, COMC},
  url          = {http://dx.doi.org/10.1111/j.1574-695X.2009.00522.x},
  volume       = {55},
  year         = {2009},
}

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