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The actin-capping protein CapG localizes to microtubule-dependent organelles during the cell cycle

Thomas Hubert (UGent) , Katrien Van Impe (UGent) , Joël Vandekerckhove (UGent) and Jan Gettemans (UGent)
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Organization
Abstract
Extensive cross-talk between the actin and the microtubule cytoskeletons has been reported. Especially In mitosis, processes dependent on actin- and microtubule-based structures alternate and regulate each other in a complex cascade leading to division into two daughter cells. Here, we have Studied the subcelluar localization of the filamentous actin-capping protein CapG. Fluorescence microscopy of endogenous CaPG and EGFP-tagged CapG revealed CapG localization at the mother centriole in interphase, the mitotic spindle in mitosis and the midbody ring in abscission. Surprisingly, nucleoporin Nup62, an interaction partner of CapG, also localized to the midbody ring at the end of abscission and colocalized with CapG. We propose a role for the actin-binding protein CapG as a mediator of cross-talk between the actin cytoskeleton and microtubule-based organelles that regulate cell division.
Keywords
Mitotic spindle, CapG, Cell cycle, Nup62, Midbody ring, Centrosome

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Citation

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Chicago
Hubert, Thomas, Katrien Van Impe, Joël Vandekerckhove, and Jan Gettemans. 2009. “The Actin-capping Protein CapG Localizes to Microtubule-dependent Organelles During the Cell Cycle.” Biochemical and Biophysical Research Communications 380 (1): 166–170.
APA
Hubert, T., Van Impe, K., Vandekerckhove, J., & Gettemans, J. (2009). The actin-capping protein CapG localizes to microtubule-dependent organelles during the cell cycle. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 380(1), 166–170.
Vancouver
1.
Hubert T, Van Impe K, Vandekerckhove J, Gettemans J. The actin-capping protein CapG localizes to microtubule-dependent organelles during the cell cycle. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. SAN DIEGO, CA 92101-4495 USA: ACADEMIC PRESS INC ELSEVIER SCIENCE; 2009;380(1):166–70.
MLA
Hubert, Thomas, Katrien Van Impe, Joël Vandekerckhove, et al. “The Actin-capping Protein CapG Localizes to Microtubule-dependent Organelles During the Cell Cycle.” BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 380.1 (2009): 166–170. Print.
@article{537371,
  abstract     = {Extensive cross-talk between the actin and the microtubule cytoskeletons has been reported. Especially In mitosis, processes dependent on actin- and microtubule-based structures alternate and regulate each other in a complex cascade leading to division into two daughter cells. Here, we have Studied the subcelluar localization of the filamentous actin-capping protein CapG. Fluorescence microscopy of endogenous CaPG and EGFP-tagged CapG revealed CapG localization at the mother centriole in interphase, the mitotic spindle in mitosis and the midbody ring in abscission. Surprisingly, nucleoporin Nup62, an interaction partner of CapG, also localized to the midbody ring at the end of abscission and colocalized with CapG. We propose a role for the actin-binding protein CapG as a mediator of cross-talk between the actin cytoskeleton and microtubule-based organelles that regulate cell division.},
  author       = {Hubert, Thomas and Van Impe, Katrien and Vandekerckhove, Jo{\"e}l and Gettemans, Jan},
  issn         = {0006-291X},
  journal      = {BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS},
  language     = {eng},
  number       = {1},
  pages        = {166--170},
  publisher    = {ACADEMIC PRESS INC ELSEVIER SCIENCE},
  title        = {The actin-capping protein CapG localizes to microtubule-dependent organelles during the cell cycle},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2009.01.064},
  volume       = {380},
  year         = {2009},
}

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