Enhanced pulmonary leptin expression in patients with severe COPD and asymptomatic smokers
- Author
- JHJ Vernooy, NEA Drummen, RJ Van Suylen, RHE Cloots, GM Moller, Ken Bracke (UGent) , S Zuyderduyn, MA Dentener, Guy Brusselle (UGent) , PS Hiemstra and EFM Wouters
- Organization
- Abstract
- Background: Chronic obstructive pulmonary disease (COPD) is characterised by an abnormal inflammatory reaction of the lungs involving activation of epithelial cells. Leptin is a pleiotropic cytokine important in the regulation of immune responses via its functional receptor Ob-Rb. This study was undertaken to test the hypothesis that severe COPD is associated with increased leptin expression in epithelial cells. Methods: Immunohistochemistry for leptin was performed on peripheral lung specimens from 20 patients with COPD (GOLD stage 4), 14 asymptomatic ex-smokers and 13 never smokers. Leptin and Ob-Rb mRNA expression were determined by rtPCR in cultured primary bronchial epithelial cells and primary type II pneumocytes. NCI-H292 and A549 cell lines were used to study functional activation of leptin signalling. Results: Leptin immunoreactivity in lung tissue was observed in bronchial epithelial cells, type II pneumocytes, macrophages (tissue/alveolar) and interstitial lymphocytic infiltrates. rtPCR analysis confirmed pulmonary leptin and Ob-Rb mRNA expression in primary bronchial epithelial cells and pneumocytes. Leptin- expressing bronchial epithelial cells and alveolar macrophages were markedly higher in patients with severe COPD and ex-smokers than in never smokers (p<0.02). Exposure of cultured primary bronchial epithelial cells to smoke resulted in increased expression of both leptin and Ob-Rb (p<0.05). Leptin induced phosphorylation of STAT3 in both NCI-H292 and A549 cells. Conclusions: Leptin expression is increased in bronchial epithelial cells and alveolar macrophages of ex-smokers with or without severe COPD compared with never smokers. A functional leptin signalling pathway is present in lung epithelial cells.
- Keywords
- starvation, pneumonia, activation, proliferation, lung, receptor, disease, host-defense, Epithelial cells, T-lymphocytes
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Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-537346
- MLA
- Vernooy, JHJ, et al. “Enhanced Pulmonary Leptin Expression in Patients with Severe COPD and Asymptomatic Smokers.” Thorax, vol. 64, no. 1, BMJ Publishing Group, 2009, pp. 26–32, doi:10.1136/thx.2007.085423.
- APA
- Vernooy, J., Drummen, N., Van Suylen, R., Cloots, R., Moller, G., Bracke, K., … Wouters, E. (2009). Enhanced pulmonary leptin expression in patients with severe COPD and asymptomatic smokers. Thorax, 64(1), 26–32. https://doi.org/10.1136/thx.2007.085423
- Chicago author-date
- Vernooy, JHJ, NEA Drummen, RJ Van Suylen, RHE Cloots, GM Moller, Ken Bracke, S Zuyderduyn, et al. 2009. “Enhanced Pulmonary Leptin Expression in Patients with Severe COPD and Asymptomatic Smokers.” Thorax 64 (1): 26–32. https://doi.org/10.1136/thx.2007.085423.
- Chicago author-date (all authors)
- Vernooy, JHJ, NEA Drummen, RJ Van Suylen, RHE Cloots, GM Moller, Ken Bracke, S Zuyderduyn, MA Dentener, Guy Brusselle, PS Hiemstra, and EFM Wouters. 2009. “Enhanced Pulmonary Leptin Expression in Patients with Severe COPD and Asymptomatic Smokers.” Thorax 64 (1): 26–32. doi:10.1136/thx.2007.085423.
- Vancouver
- 1.Vernooy J, Drummen N, Van Suylen R, Cloots R, Moller G, Bracke K, et al. Enhanced pulmonary leptin expression in patients with severe COPD and asymptomatic smokers. Thorax. 2009;64(1):26–32.
- IEEE
- [1]J. Vernooy et al., “Enhanced pulmonary leptin expression in patients with severe COPD and asymptomatic smokers,” Thorax, vol. 64, no. 1, pp. 26–32, 2009.
@article{537346, abstract = {{Background: Chronic obstructive pulmonary disease (COPD) is characterised by an abnormal inflammatory reaction of the lungs involving activation of epithelial cells. Leptin is a pleiotropic cytokine important in the regulation of immune responses via its functional receptor Ob-Rb. This study was undertaken to test the hypothesis that severe COPD is associated with increased leptin expression in epithelial cells. Methods: Immunohistochemistry for leptin was performed on peripheral lung specimens from 20 patients with COPD (GOLD stage 4), 14 asymptomatic ex-smokers and 13 never smokers. Leptin and Ob-Rb mRNA expression were determined by rtPCR in cultured primary bronchial epithelial cells and primary type II pneumocytes. NCI-H292 and A549 cell lines were used to study functional activation of leptin signalling. Results: Leptin immunoreactivity in lung tissue was observed in bronchial epithelial cells, type II pneumocytes, macrophages (tissue/alveolar) and interstitial lymphocytic infiltrates. rtPCR analysis confirmed pulmonary leptin and Ob-Rb mRNA expression in primary bronchial epithelial cells and pneumocytes. Leptin- expressing bronchial epithelial cells and alveolar macrophages were markedly higher in patients with severe COPD and ex-smokers than in never smokers (p<0.02). Exposure of cultured primary bronchial epithelial cells to smoke resulted in increased expression of both leptin and Ob-Rb (p<0.05). Leptin induced phosphorylation of STAT3 in both NCI-H292 and A549 cells. Conclusions: Leptin expression is increased in bronchial epithelial cells and alveolar macrophages of ex-smokers with or without severe COPD compared with never smokers. A functional leptin signalling pathway is present in lung epithelial cells.}}, author = {{Vernooy, JHJ and Drummen, NEA and Van Suylen, RJ and Cloots, RHE and Moller, GM and Bracke, Ken and Zuyderduyn, S and Dentener, MA and Brusselle, Guy and Hiemstra, PS and Wouters, EFM}}, issn = {{0040-6376}}, journal = {{Thorax}}, keywords = {{starvation,pneumonia,activation,proliferation,lung,receptor,disease,host-defense,Epithelial cells,T-lymphocytes}}, language = {{eng}}, number = {{1}}, pages = {{26--32}}, publisher = {{BMJ Publishing Group}}, title = {{Enhanced pulmonary leptin expression in patients with severe COPD and asymptomatic smokers}}, url = {{http://doi.org/10.1136/thx.2007.085423}}, volume = {{64}}, year = {{2009}}, }
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