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Suspension fluorescence in situ hybridization (S-FISH) combined with automatic detection and laser microdissection for STR profiling of male cells in male/female mixtures

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Abstract
Laser microdissection is a valuable tool for isolating specific cells from mixtures, such as male cells in a mixture with female cells, e. g., in cases of sexual assault. These cells can be stained with Y-chromosomespecific probes. We developed an automatic screening method to detect male cells after fluorescence in situ hybridization in suspension (S-FISH). To simulate forensic casework, the method was tested on female saliva after cataglottis (a kiss involving tongue-to-tongue contact) and on licking traces (swabs of dried male saliva on female skin) even after drying. After isolation of the detected cells, short tandem repeat profiling was performed. Full DNA profiles could consistently be obtained from as little as ten buccal cells. Isolation of five cells resulted in a mean of 98% (SD of 3.4%) of the alleles detected, showing that the developed S-FISH staining had no significant negative influence on DNA recovery and can be used in forensic casework.
Keywords
Hybridization, Male non-sperm cells, Automatic cell recognition, SEXUAL ASSAULT, CAPTURE MICRODISSECTION, GENDER IDENTIFICATION, DNA, SPERMATOZOA, SPERM, Forensic science, Laser capture microdissection

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Citation

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Chicago
Vandewoestyne, Mado, David Van Hoofstat, Filip Van Nieuwerburgh, and Dieter Deforce. 2009. “Suspension Fluorescence in Situ Hybridization (S-FISH) Combined with Automatic Detection and Laser Microdissection for STR Profiling of Male Cells in Male/female Mixtures.” International Journal of Legal Medicine 123 (5): 441–447.
APA
Vandewoestyne, M., Van Hoofstat, D., Van Nieuwerburgh, F., & Deforce, D. (2009). Suspension fluorescence in situ hybridization (S-FISH) combined with automatic detection and laser microdissection for STR profiling of male cells in male/female mixtures. INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 123(5), 441–447.
Vancouver
1.
Vandewoestyne M, Van Hoofstat D, Van Nieuwerburgh F, Deforce D. Suspension fluorescence in situ hybridization (S-FISH) combined with automatic detection and laser microdissection for STR profiling of male cells in male/female mixtures. INTERNATIONAL JOURNAL OF LEGAL MEDICINE. 2009;123(5):441–7.
MLA
Vandewoestyne, Mado et al. “Suspension Fluorescence in Situ Hybridization (S-FISH) Combined with Automatic Detection and Laser Microdissection for STR Profiling of Male Cells in Male/female Mixtures.” INTERNATIONAL JOURNAL OF LEGAL MEDICINE 123.5 (2009): 441–447. Print.
@article{535148,
  abstract     = {Laser microdissection is a valuable tool for isolating specific cells from mixtures, such as male cells in a mixture with female cells, e. g., in cases of sexual assault. These cells can be stained with Y-chromosomespecific probes. We developed an automatic screening method to detect male cells after fluorescence in situ hybridization in suspension (S-FISH). To simulate forensic casework, the method was tested on female saliva after cataglottis (a kiss involving tongue-to-tongue contact) and on licking traces (swabs of dried male saliva on female skin) even after drying. After isolation of the detected cells, short tandem repeat profiling was performed. Full DNA profiles could consistently be obtained from as little as ten buccal cells. Isolation of five cells resulted in a mean of 98\% (SD of 3.4\%) of the alleles detected, showing that the developed S-FISH staining had no significant negative influence on DNA recovery and can be used in forensic casework.},
  author       = {Vandewoestyne, Mado and Van Hoofstat, David and Van Nieuwerburgh, Filip and Deforce, Dieter},
  issn         = {0937-9827},
  journal      = {INTERNATIONAL JOURNAL OF LEGAL MEDICINE},
  language     = {eng},
  number       = {5},
  pages        = {441--447},
  title        = {Suspension fluorescence in situ hybridization (S-FISH) combined with automatic detection and laser microdissection for STR profiling of male cells in male/female mixtures},
  url          = {http://dx.doi.org/10.1007/s00414-009-0341-z},
  volume       = {123},
  year         = {2009},
}

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