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Evaluation of an injectable, photopolymerizable, and three-dimensional scaffold based on methacrylate-endcapped poly(D,L-lactide-co-ε-caprolactone) combined with autologous mesenchymal stem cells in a goat tibial unicortical defect model

Geert Vertenten (UGent) , Evi Lippens (UGent) , Jordi Gironès Molera (UGent) , Tomasz Gorski, Heidi Declercq (UGent) , Jimmy Saunders (UGent) , Wim Van Den Broeck (UGent) , Koen Chiers (UGent) , Luc Duchateau (UGent) , Etienne Schacht (UGent) , et al.
(2009) TISSUE ENGINEERING PART A. 15(7). p.1501-1511
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Abstract
An in situ crosslinkable, biodegradable, methacrylate-endcapped poly(D,L-lactide-co-epsilon-caprolactone) in which crosslinkage is achieved by photoinitiators was developed for bone tissue regeneration. Different combinations of the polymer with bone marrow-derived mesenchymal stem cells (BMSCs) and alpha-tricalcium phosphate (alpha-TCP) were tested in a unicortical tibial defect model in eight goats. The polymers were randomly applied in one of three defects (6.0 mm diameter) using a fourth unfilled defect as control. Biocompatibility and bone-healing characteristics were evaluated by serial radiographies, histology, histomorphometry, and immunohistochemistry. The results demonstrated cell survival and proliferation in the polymer-substituted bone defects. The addition of alpha-TCP was associated with less expansion and growth of the BMSCs than other polymer composites.
Keywords
NETWORKS, MICROCARRIERS, CHONDROGENESIS, REPAIR, HYDROGEL, TRICALCIUM PHOSPHATE, SEGMENTAL DEFECT, TISSUE-ENGINEERED BONE, EPSILON-CAPROLACTONE, MARROW STROMAL CELLS

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Chicago
Vertenten, Geert, Evi Lippens, Jordi Gironès Molera, Tomasz Gorski, Heidi Declercq, Jimmy Saunders, Wim Van Den Broeck, et al. 2009. “Evaluation of an Injectable, Photopolymerizable, and Three-dimensional Scaffold Based on Methacrylate-endcapped poly(D,L-lactide-co-ε-caprolactone) Combined with Autologous Mesenchymal Stem Cells in a Goat Tibial Unicortical Defect Model.” Tissue Engineering Part A 15 (7): 1501–1511.
APA
Vertenten, G., Lippens, E., Gironès Molera, J., Gorski, T., Declercq, H., Saunders, J., Van Den Broeck, W., et al. (2009). Evaluation of an injectable, photopolymerizable, and three-dimensional scaffold based on methacrylate-endcapped poly(D,L-lactide-co-ε-caprolactone) combined with autologous mesenchymal stem cells in a goat tibial unicortical defect model. TISSUE ENGINEERING PART A, 15(7), 1501–1511.
Vancouver
1.
Vertenten G, Lippens E, Gironès Molera J, Gorski T, Declercq H, Saunders J, et al. Evaluation of an injectable, photopolymerizable, and three-dimensional scaffold based on methacrylate-endcapped poly(D,L-lactide-co-ε-caprolactone) combined with autologous mesenchymal stem cells in a goat tibial unicortical defect model. TISSUE ENGINEERING PART A. 2009;15(7):1501–11.
MLA
Vertenten, Geert, Evi Lippens, Jordi Gironès Molera, et al. “Evaluation of an Injectable, Photopolymerizable, and Three-dimensional Scaffold Based on Methacrylate-endcapped poly(D,L-lactide-co-ε-caprolactone) Combined with Autologous Mesenchymal Stem Cells in a Goat Tibial Unicortical Defect Model.” TISSUE ENGINEERING PART A 15.7 (2009): 1501–1511. Print.
@article{534488,
  abstract     = {An in situ crosslinkable, biodegradable, methacrylate-endcapped poly(D,L-lactide-co-epsilon-caprolactone) in which crosslinkage is achieved by photoinitiators was developed for bone tissue regeneration. Different combinations of the polymer with bone marrow-derived mesenchymal stem cells (BMSCs) and alpha-tricalcium phosphate (alpha-TCP) were tested in a unicortical tibial defect model in eight goats. The polymers were randomly applied in one of three defects (6.0 mm diameter) using a fourth unfilled defect as control. Biocompatibility and bone-healing characteristics were evaluated by serial radiographies, histology, histomorphometry, and immunohistochemistry. The results demonstrated cell survival and proliferation in the polymer-substituted bone defects. The addition of alpha-TCP was associated with less expansion and growth of the BMSCs than other polymer composites.},
  author       = {Vertenten, Geert and Lippens, Evi and Giron{\`e}s Molera, Jordi and Gorski, Tomasz and Declercq, Heidi and Saunders, Jimmy and Van Den Broeck, Wim and Chiers, Koen and Duchateau, Luc and Schacht, Etienne and Cornelissen, Maria and Gasthuys, Frank and Vlaminck, Lieven},
  issn         = {1937-3341},
  journal      = {TISSUE ENGINEERING PART A},
  keyword      = {NETWORKS,MICROCARRIERS,CHONDROGENESIS,REPAIR,HYDROGEL,TRICALCIUM PHOSPHATE,SEGMENTAL DEFECT,TISSUE-ENGINEERED BONE,EPSILON-CAPROLACTONE,MARROW STROMAL CELLS},
  language     = {eng},
  number       = {7},
  pages        = {1501--1511},
  title        = {Evaluation of an injectable, photopolymerizable, and three-dimensional scaffold based on methacrylate-endcapped poly(D,L-lactide-co-\ensuremath{\epsilon}-caprolactone) combined with autologous mesenchymal stem cells in a goat tibial unicortical defect model},
  url          = {http://dx.doi.org/10.1089/ten.tea.2008.0367},
  volume       = {15},
  year         = {2009},
}

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