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Bacteria that inhibit quorum sensing decrease biofilm formation and virulence in Pseudomonas aeruginosa PAO1

Steven Christiaen, Nele Matthijs, Xiao-Hua Zhang, Hans Nelis UGent, Peter Bossier UGent and Tom Coenye UGent (2014) PATHOGENS AND DISEASE. 70(3). p.271-279
abstract
In this study, we investigated the biotherapeutic potential of previously isolated quorum quenching (QQ) bacteria. Some of them produce and secrete small compounds that inhibit quorum sensing (QS), others quench QS by enzymatic degradation of N-acylhomoserine lactones (AHLs). The supernatant of cultures of these isolates was tested for inhibitory properties against P.aeruginosa PAO1 biofilms. Most isolates had a moderate effect on biofilm formation, as shown by viability staining and/or staining of the biofilm biomass. A substantial part of the isolates reduced P.aeruginosa elastase production in a concentration-dependent manner. Using Caenorhabditis elegans as an in vivo model system for virulence testing, we found that some of the isolates were able to increase survival of P.aeruginosa PAO1 and Burkholderia cenocepacia LGM16656-infected nematodes when co-administered with the pathogen. Altogether, these data indicate that some QQ bacteria, or the active compounds they produce, could be useful to attenuate virulence of P.aeruginosa PAO1 and possibly also other Gram-negative pathogens that use AHLs to regulate the production of virulence factors.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
quorum quenching, QUENCHING BACTERIA, biofilm, GENE-EXPRESSION, IN-VITRO, CAENORHABDITIS-ELEGANS, Pseudomonas aeruginosa, ACYL-HOMOSERINE LACTONE, INFECTIONS, PROBIOTICS, SUSCEPTIBILITY, IDENTIFICATION, RHIZOSPHERE, virulence, elastase, Caenorhabditis elegans
journal title
PATHOGENS AND DISEASE
Pathog. Dis.
volume
70
issue
3
pages
271 - 279
Web of Science type
Article
Web of Science id
000334124300009
JCR category
MICROBIOLOGY
JCR impact factor
2.403 (2014)
JCR rank
67/119 (2014)
JCR quartile
3 (2014)
ISSN
2049-632X
DOI
10.1111/2049-632X.12124
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5332551
handle
http://hdl.handle.net/1854/LU-5332551
date created
2014-07-04 10:15:28
date last changed
2016-12-19 15:39:08
@article{5332551,
  abstract     = {In this study, we investigated the biotherapeutic potential of previously isolated quorum quenching (QQ) bacteria. Some of them produce and secrete small compounds that inhibit quorum sensing (QS), others quench QS by enzymatic degradation of N-acylhomoserine lactones (AHLs). The supernatant of cultures of these isolates was tested for inhibitory properties against P.aeruginosa PAO1 biofilms. Most isolates had a moderate effect on biofilm formation, as shown by viability staining and/or staining of the biofilm biomass. A substantial part of the isolates reduced P.aeruginosa elastase production in a concentration-dependent manner. Using Caenorhabditis elegans as an in vivo model system for virulence testing, we found that some of the isolates were able to increase survival of P.aeruginosa PAO1 and Burkholderia cenocepacia LGM16656-infected nematodes when co-administered with the pathogen. Altogether, these data indicate that some QQ bacteria, or the active compounds they produce, could be useful to attenuate virulence of P.aeruginosa PAO1 and possibly also other Gram-negative pathogens that use AHLs to regulate the production of virulence factors.},
  author       = {Christiaen, Steven and Matthijs, Nele and Zhang, Xiao-Hua and Nelis, Hans and Bossier, Peter and Coenye, Tom},
  issn         = {2049-632X},
  journal      = {PATHOGENS AND DISEASE},
  keyword      = {quorum quenching,QUENCHING BACTERIA,biofilm,GENE-EXPRESSION,IN-VITRO,CAENORHABDITIS-ELEGANS,Pseudomonas aeruginosa,ACYL-HOMOSERINE LACTONE,INFECTIONS,PROBIOTICS,SUSCEPTIBILITY,IDENTIFICATION,RHIZOSPHERE,virulence,elastase,Caenorhabditis elegans},
  language     = {eng},
  number       = {3},
  pages        = {271--279},
  title        = {Bacteria that inhibit quorum sensing decrease biofilm formation and virulence in Pseudomonas aeruginosa PAO1},
  url          = {http://dx.doi.org/10.1111/2049-632X.12124},
  volume       = {70},
  year         = {2014},
}

Chicago
Christiaen, Steven, Nele Matthijs, Xiao-Hua Zhang, Hans Nelis, Peter Bossier, and Tom Coenye. 2014. “Bacteria That Inhibit Quorum Sensing Decrease Biofilm Formation and Virulence in Pseudomonas Aeruginosa PAO1.” Pathogens and Disease 70 (3): 271–279.
APA
Christiaen, S., Matthijs, N., Zhang, X.-H., Nelis, H., Bossier, P., & Coenye, T. (2014). Bacteria that inhibit quorum sensing decrease biofilm formation and virulence in Pseudomonas aeruginosa PAO1. PATHOGENS AND DISEASE, 70(3), 271–279.
Vancouver
1.
Christiaen S, Matthijs N, Zhang X-H, Nelis H, Bossier P, Coenye T. Bacteria that inhibit quorum sensing decrease biofilm formation and virulence in Pseudomonas aeruginosa PAO1. PATHOGENS AND DISEASE. 2014;70(3):271–9.
MLA
Christiaen, Steven, Nele Matthijs, Xiao-Hua Zhang, et al. “Bacteria That Inhibit Quorum Sensing Decrease Biofilm Formation and Virulence in Pseudomonas Aeruginosa PAO1.” PATHOGENS AND DISEASE 70.3 (2014): 271–279. Print.