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Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003

(2014) PLOS ONE. 9(5).
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Abstract
In the present study we show that luxS of Bifidobacterium breve UCC2003 is involved in the production of the interspecies signaling molecule autoinducer-2 (AI-2), and that this gene is essential for gastrointestinal colonization of a murine host, while it is also involved in providing protection against Salmonella infection in Caenorhabditis elegans. We demonstrate that a B. breve luxS-insertion mutant is significantly more susceptible to iron chelators than the WT strain and that this sensitivity can be partially reverted in the presence of the AI-2 precursor DPD. Furthermore, we show that several genes of an iron starvation-induced gene cluster, which are downregulated in the luxS-insertion mutant and which encodes a presumed iron-uptake system, are transcriptionally upregulated under in vivo conditions. Mutation of two genes of this cluster in B. breve UCC2003 renders the derived mutant strains sensitive to iron chelators while deficient in their ability to confer gut pathogen protection to Salmonella-infected nematodes. Since a functional luxS gene is present in all tested members of the genus Bifidobacterium, we conclude that bifidobacteria operate a LuxS-mediated system for gut colonization and pathogen protection that is correlated with iron acquisition.
Keywords
SALMONELLA-TYPHIMURIUM, ESCHERICHIA-COLI, PSEUDOMONAS-AERUGINOSA, GENE-EXPRESSION, NECROTIZING ENTEROCOLITIS, CAENORHABDITIS-ELEGANS, QUORUM-SENSING ROLE, LACTOBACILLUS-RHAMNOSUS GG, INFLAMMATORY-BOWEL-DISEASE, DNA-MICROARRAY DATA

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Chicago
Christiaen, Steven, Mary O’Connell Motherway, Francesca Bottacini, Noreen Lanigan, Pat G Casey, Geert Huys, Hans Nelis, Douwe van Sinderen, and Tom Coenye. 2014. “Autoinducer-2 Plays a Crucial Role in Gut Colonization and Probiotic Functionality of Bifidobacterium Breve UCC2003.” Plos One 9 (5).
APA
Christiaen, S., Motherway, M. O., Bottacini, F., Lanigan, N., Casey, P. G., Huys, G., Nelis, H., et al. (2014). Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003. PLOS ONE, 9(5).
Vancouver
1.
Christiaen S, Motherway MO, Bottacini F, Lanigan N, Casey PG, Huys G, et al. Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003. PLOS ONE. 2014;9(5).
MLA
Christiaen, Steven, Mary O’Connell Motherway, Francesca Bottacini, et al. “Autoinducer-2 Plays a Crucial Role in Gut Colonization and Probiotic Functionality of Bifidobacterium Breve UCC2003.” PLOS ONE 9.5 (2014): n. pag. Print.
@article{5332515,
  abstract     = {In the present study we show that luxS of Bifidobacterium breve UCC2003 is involved in the production of the interspecies signaling molecule autoinducer-2 (AI-2), and that this gene is essential for gastrointestinal colonization of a murine host, while it is also involved in providing protection against Salmonella infection in Caenorhabditis elegans. We demonstrate that a B. breve luxS-insertion mutant is significantly more susceptible to iron chelators than the WT strain and that this sensitivity can be partially reverted in the presence of the AI-2 precursor DPD. Furthermore, we show that several genes of an iron starvation-induced gene cluster, which are downregulated in the luxS-insertion mutant and which encodes a presumed iron-uptake system, are transcriptionally upregulated under in vivo conditions. Mutation of two genes of this cluster in B. breve UCC2003 renders the derived mutant strains sensitive to iron chelators while deficient in their ability to confer gut pathogen protection to Salmonella-infected nematodes. Since a functional luxS gene is present in all tested members of the genus Bifidobacterium, we conclude that bifidobacteria operate a LuxS-mediated system for gut colonization and pathogen protection that is correlated with iron acquisition.},
  articleno    = {e98111},
  author       = {Christiaen, Steven and Motherway, Mary O'Connell and Bottacini, Francesca and Lanigan, Noreen and Casey, Pat G and Huys, Geert and Nelis, Hans and van Sinderen, Douwe and Coenye, Tom},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  language     = {eng},
  number       = {5},
  pages        = {13},
  title        = {Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003},
  url          = {http://dx.doi.org/10.1371/journal.pone.0098111},
  volume       = {9},
  year         = {2014},
}

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