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Web-based scoring of the dicentric assay, a collaborative biodosimetric scoring strategy for population triage in large scale radiation accidents

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Abstract
In the case of a large scale radiation accident high throughput methods of biological dosimetry for population triage are needed to identify individuals requiring clinical treatment. The dicentric assay performed in web-based scoring mode may be a very suitable technique. Within the MULTIBIODOSE EU FP7 project a network is being established of 8 laboratories with expertise in dose estimations based on the dicentric assay. Here, the manual dicentric assay was tested in a web-based scoring mode. More than 23,000 high resolution images of metaphase spreads (only first mitosis) were captured by four laboratories and established as image galleries on the internet (cloud). The galleries included images of a complete dose effect curve (0-5.0 Gy) and three types of irradiation scenarios simulating acute whole body, partial body and protracted exposure. The blood samples had been irradiated in vitro with gamma rays at the University of Ghent, Belgium. Two laboratories provided image galleries from Fluorescence plus Giemsa stained slides (3 h colcemid) and the image galleries from the other two laboratories contained images from Giemsa stained preparations (24 h colcemid). Each of the 8 participating laboratories analysed 3 dose points of the dose effect curve (scoring 100 cells for each point) and 3 unknown dose points (50 cells) for each of the 3 simulated irradiation scenarios. At first all analyses were performed in a QuickScan Mode without scoring individual chromosomes, followed by conventional scoring (only complete cells, 46 centromeres). The calibration curves obtained using these two scoring methods were very similar, with no significant difference in the linear-quadratic curve coefficients. Analysis of variance showed a significant effect of dose on the yield of dicentrics, but no significant effect of the laboratories, different methods of slide preparation or different incubation times used for colcemid. The results obtained to date within the MULTIBIODOSE project by a network of 8 collaborating laboratories throughout Europe are very promising. The dicentric assay in the web based scoring mode as a high throughput scoring strategy is a useful application for biodosimetry in the case of a large scale radiation accident.
Keywords
LABORATORIES, CAPACITY, BIODOSENET, CASUALTIES, NETWORK, CHROMOSOME ANALYSIS, EMERGENCY, BIOLOGICAL DOSIMETRY, Emergency preparedness, Radiation dose assessment, Radiation accident, Web based scoring, Biological dosimetry, Dicentric assay

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MLA
Romm, H, E Ainsbury, A Bajinskis, et al. “Web-based Scoring of the Dicentric Assay, a Collaborative Biodosimetric Scoring Strategy for Population Triage in Large Scale Radiation Accidents.” RADIATION AND ENVIRONMENTAL BIOPHYSICS 53.2 (2014): 241–254. Print.
APA
Romm, H., Ainsbury, E., Bajinskis, A., Barnard, S., Barquinero, J., Barrios, L., Beinke, C., et al. (2014). Web-based scoring of the dicentric assay, a collaborative biodosimetric scoring strategy for population triage in large scale radiation accidents. RADIATION AND ENVIRONMENTAL BIOPHYSICS, 53(2), 241–254.
Chicago author-date
Romm, H, E Ainsbury, A Bajinskis, S Barnard, JF Barquinero, L Barrios, C Beinke, et al. 2014. “Web-based Scoring of the Dicentric Assay, a Collaborative Biodosimetric Scoring Strategy for Population Triage in Large Scale Radiation Accidents.” Radiation and Environmental Biophysics 53 (2): 241–254.
Chicago author-date (all authors)
Romm, H, E Ainsbury, A Bajinskis, S Barnard, JF Barquinero, L Barrios, C Beinke, R Puig-Casanovas, M Deperas-Kaminska, E Gregoire, U Oestreicher, C Lindholm, J Moquet, K Rothkamm, S Sommer, Hubert Thierens, Anne Vral, Veerle Vandersickel, and A Wojcik. 2014. “Web-based Scoring of the Dicentric Assay, a Collaborative Biodosimetric Scoring Strategy for Population Triage in Large Scale Radiation Accidents.” Radiation and Environmental Biophysics 53 (2): 241–254.
Vancouver
1.
Romm H, Ainsbury E, Bajinskis A, Barnard S, Barquinero J, Barrios L, et al. Web-based scoring of the dicentric assay, a collaborative biodosimetric scoring strategy for population triage in large scale radiation accidents. RADIATION AND ENVIRONMENTAL BIOPHYSICS. 2014;53(2):241–54.
IEEE
[1]
H. Romm et al., “Web-based scoring of the dicentric assay, a collaborative biodosimetric scoring strategy for population triage in large scale radiation accidents,” RADIATION AND ENVIRONMENTAL BIOPHYSICS, vol. 53, no. 2, pp. 241–254, 2014.
@article{5332398,
  abstract     = {In the case of a large scale radiation accident high throughput methods of biological dosimetry for population triage are needed to identify individuals requiring clinical treatment. The dicentric assay performed in web-based scoring mode may be a very suitable technique. Within the MULTIBIODOSE EU FP7 project a network is being established of 8 laboratories with expertise in dose estimations based on the dicentric assay. Here, the manual dicentric assay was tested in a web-based scoring mode. More than 23,000 high resolution images of metaphase spreads (only first mitosis) were captured by four laboratories and established as image galleries on the internet (cloud). The galleries included images of a complete dose effect curve (0-5.0 Gy) and three types of irradiation scenarios simulating acute whole body, partial body and protracted exposure. The blood samples had been irradiated in vitro with gamma rays at the University of Ghent, Belgium. Two laboratories provided image galleries from Fluorescence plus Giemsa stained slides (3 h colcemid) and the image galleries from the other two laboratories contained images from Giemsa stained preparations (24 h colcemid). Each of the 8 participating laboratories analysed 3 dose points of the dose effect curve (scoring 100 cells for each point) and 3 unknown dose points (50 cells) for each of the 3 simulated irradiation scenarios. At first all analyses were performed in a QuickScan Mode without scoring individual chromosomes, followed by conventional scoring (only complete cells, 46 centromeres). The calibration curves obtained using these two scoring methods were very similar, with no significant difference in the linear-quadratic curve coefficients. Analysis of variance showed a significant effect of dose on the yield of dicentrics, but no significant effect of the laboratories, different methods of slide preparation or different incubation times used for colcemid. The results obtained to date within the MULTIBIODOSE project by a network of 8 collaborating laboratories throughout Europe are very promising. The dicentric assay in the web based scoring mode as a high throughput scoring strategy is a useful application for biodosimetry in the case of a large scale radiation accident.},
  author       = {Romm, H and Ainsbury, E and Bajinskis, A and Barnard, S and Barquinero, JF and Barrios, L and Beinke, C and Puig-Casanovas, R and Deperas-Kaminska, M and Gregoire, E and Oestreicher, U and Lindholm, C and Moquet, J and Rothkamm, K and Sommer, S and Thierens, Hubert and Vral, Anne and Vandersickel, Veerle and Wojcik, A},
  issn         = {0301-634X},
  journal      = {RADIATION AND ENVIRONMENTAL BIOPHYSICS},
  keywords     = {LABORATORIES,CAPACITY,BIODOSENET,CASUALTIES,NETWORK,CHROMOSOME ANALYSIS,EMERGENCY,BIOLOGICAL DOSIMETRY,Emergency preparedness,Radiation dose assessment,Radiation accident,Web based scoring,Biological dosimetry,Dicentric assay},
  language     = {eng},
  number       = {2},
  pages        = {241--254},
  title        = {Web-based scoring of the dicentric assay, a collaborative biodosimetric scoring strategy for population triage in large scale radiation accidents},
  url          = {http://dx.doi.org/10.1007/s00411-014-0519-8},
  volume       = {53},
  year         = {2014},
}

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