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C1 metabolism and chlorophyll synthesis: the Mg-protoporphyrin IX methyltransferase activity is dependent on the folate status

Valérie Van Wilder, Veerle De Brouwer UGent, Karen Loizeau, Bernadette Gambonnet, Catherine Albrieux, Dominique Van Der Straeten UGent, Willy Lambert, Roland Douce, Maryse A. Block, Fabrice Rebeille, et al. (2009) NEW PHYTOLOGIST. 182(1). p.137-145
abstract
Tetrahydrofolate derivatives are central cofactors of C1 metabolism. Using methotrexate as a specific inhibitor of folate biosynthesis, we altered the folate status in 10-d-old etiolated pea (Pisum sativum) leaves and followed the rate of chlorophyll synthesis upon illumination. In our conditions, the folate concentration decreased only from 5.7 to 4.2 nmol g(-1) FW, but the amount of chlorophyll after 24 h of illumination was reduced 2.5 times. Folate status and rate of chlorophyll synthesis were apparently correlated through the methyl cycle. Indeed, we observed that methyl-tetrahydrofolate was the folate derivative most affected by the treatment; the decrease of methyl-tetrahydrofolate was associated with a sharp rise in homocysteine and S-adenosylhomocysteine concentrations, which are normally maintained at very low values, shifting the methylation index (S-adenosylmethionine/S-adenosylhomocysteine ratio) from 7 to 1; the decrease of the methylation index reduced by a factor of 3 the activity of the Mg-protoporphyrin IX methyltransferase (CHLM), an essential enzyme for chlorophyll synthesis. CHLM gene expression and protein concentration remained unchanged, suggesting that this inhibition relied essentially on metabolic regulation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARABIDOPSIS, EXPRESSION, PLANT DEVELOPMENT, GLYCINE DECARBOXYLASE, S-ADENOSYLMETHIONINE, ONE-CARBON METABOLISM, ADENOSYL-L-METHIONINE, ENHANCEMENT, CYSTATHIONINE GAMMA-SYNTHASE, GENE
journal title
NEW PHYTOLOGIST
New Phytol.
volume
182
issue
1
pages
137 - 145
Web of Science type
Article
Web of Science id
000263910700015
JCR category
PLANT SCIENCES
JCR impact factor
6.033 (2009)
JCR rank
8/172 (2009)
JCR quartile
1 (2009)
ISSN
0028-646X
DOI
10.1111/j.1469-8137.2008.02707.x
language
English
UGent publication?
yes
classification
A1
id
533109
handle
http://hdl.handle.net/1854/LU-533109
date created
2009-03-27 15:58:19
date last changed
2016-12-19 15:40:29
@article{533109,
  abstract     = {Tetrahydrofolate derivatives are central cofactors of C1 metabolism. Using methotrexate as a specific inhibitor of folate biosynthesis, we altered the folate status in 10-d-old etiolated pea (Pisum sativum) leaves and followed the rate of chlorophyll synthesis upon illumination. 
In our conditions, the folate concentration decreased only from 5.7 to 4.2 nmol g(-1) FW, but the amount of chlorophyll after 24 h of illumination was reduced 2.5 times. Folate status and rate of chlorophyll synthesis were apparently correlated through the methyl cycle.
Indeed, we observed that methyl-tetrahydrofolate was the folate derivative most affected by the treatment; the decrease of methyl-tetrahydrofolate was associated with a sharp rise in homocysteine and S-adenosylhomocysteine concentrations, which are normally maintained at very low values, shifting the methylation index (S-adenosylmethionine/S-adenosylhomocysteine ratio) from 7 to 1; the decrease of the methylation index reduced by a factor of 3 the activity of the Mg-protoporphyrin IX methyltransferase (CHLM), an essential enzyme for chlorophyll synthesis. CHLM gene expression and protein concentration remained unchanged, suggesting that this inhibition relied essentially on metabolic regulation.},
  author       = {Van Wilder, Val{\'e}rie and De Brouwer, Veerle and Loizeau, Karen and Gambonnet, Bernadette and Albrieux, Catherine and Van Der Straeten, Dominique and Lambert, Willy and Douce, Roland and Block, Maryse A. and Rebeille, Fabrice and Ravanel, Stephane},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  keyword      = {ARABIDOPSIS,EXPRESSION,PLANT DEVELOPMENT,GLYCINE DECARBOXYLASE,S-ADENOSYLMETHIONINE,ONE-CARBON METABOLISM,ADENOSYL-L-METHIONINE,ENHANCEMENT,CYSTATHIONINE GAMMA-SYNTHASE,GENE},
  language     = {eng},
  number       = {1},
  pages        = {137--145},
  title        = {C1 metabolism and chlorophyll synthesis: the Mg-protoporphyrin IX methyltransferase activity is dependent on the folate status},
  url          = {http://dx.doi.org/10.1111/j.1469-8137.2008.02707.x},
  volume       = {182},
  year         = {2009},
}

Chicago
Van Wilder, Valérie, Veerle De Brouwer, Karen Loizeau, Bernadette Gambonnet, Catherine Albrieux, Dominique Van Der Straeten, Willy Lambert, et al. 2009. “C1 Metabolism and Chlorophyll Synthesis: The Mg-protoporphyrin IX Methyltransferase Activity Is Dependent on the Folate Status.” New Phytologist 182 (1): 137–145.
APA
Van Wilder, V., De Brouwer, V., Loizeau, K., Gambonnet, B., Albrieux, C., Van Der Straeten, D., Lambert, W., et al. (2009). C1 metabolism and chlorophyll synthesis: the Mg-protoporphyrin IX methyltransferase activity is dependent on the folate status. NEW PHYTOLOGIST, 182(1), 137–145.
Vancouver
1.
Van Wilder V, De Brouwer V, Loizeau K, Gambonnet B, Albrieux C, Van Der Straeten D, et al. C1 metabolism and chlorophyll synthesis: the Mg-protoporphyrin IX methyltransferase activity is dependent on the folate status. NEW PHYTOLOGIST. 2009;182(1):137–45.
MLA
Van Wilder, Valérie, Veerle De Brouwer, Karen Loizeau, et al. “C1 Metabolism and Chlorophyll Synthesis: The Mg-protoporphyrin IX Methyltransferase Activity Is Dependent on the Folate Status.” NEW PHYTOLOGIST 182.1 (2009): 137–145. Print.