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C1 metabolism and chlorophyll synthesis: the Mg-protoporphyrin IX methyltransferase activity is dependent on the folate status

(2009) NEW PHYTOLOGIST. 182(1). p.137-145
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Organization
Abstract
Tetrahydrofolate derivatives are central cofactors of C1 metabolism. Using methotrexate as a specific inhibitor of folate biosynthesis, we altered the folate status in 10-d-old etiolated pea (Pisum sativum) leaves and followed the rate of chlorophyll synthesis upon illumination. In our conditions, the folate concentration decreased only from 5.7 to 4.2 nmol g(-1) FW, but the amount of chlorophyll after 24 h of illumination was reduced 2.5 times. Folate status and rate of chlorophyll synthesis were apparently correlated through the methyl cycle. Indeed, we observed that methyl-tetrahydrofolate was the folate derivative most affected by the treatment; the decrease of methyl-tetrahydrofolate was associated with a sharp rise in homocysteine and S-adenosylhomocysteine concentrations, which are normally maintained at very low values, shifting the methylation index (S-adenosylmethionine/S-adenosylhomocysteine ratio) from 7 to 1; the decrease of the methylation index reduced by a factor of 3 the activity of the Mg-protoporphyrin IX methyltransferase (CHLM), an essential enzyme for chlorophyll synthesis. CHLM gene expression and protein concentration remained unchanged, suggesting that this inhibition relied essentially on metabolic regulation.
Keywords
ARABIDOPSIS, EXPRESSION, PLANT DEVELOPMENT, GLYCINE DECARBOXYLASE, S-ADENOSYLMETHIONINE, ONE-CARBON METABOLISM, ADENOSYL-L-METHIONINE, ENHANCEMENT, CYSTATHIONINE GAMMA-SYNTHASE, GENE

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Citation

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MLA
Van Wilder, Valérie, Veerle De Brouwer, Karen Loizeau, et al. “C1 Metabolism and Chlorophyll Synthesis: The Mg-protoporphyrin IX Methyltransferase Activity Is Dependent on the Folate Status.” NEW PHYTOLOGIST 182.1 (2009): 137–145. Print.
APA
Van Wilder, V., De Brouwer, V., Loizeau, K., Gambonnet, B., Albrieux, C., Van Der Straeten, D., Lambert, W., et al. (2009). C1 metabolism and chlorophyll synthesis: the Mg-protoporphyrin IX methyltransferase activity is dependent on the folate status. NEW PHYTOLOGIST, 182(1), 137–145.
Chicago author-date
Van Wilder, Valérie, Veerle De Brouwer, Karen Loizeau, Bernadette Gambonnet, Catherine Albrieux, Dominique Van Der Straeten, Willy Lambert, et al. 2009. “C1 Metabolism and Chlorophyll Synthesis: The Mg-protoporphyrin IX Methyltransferase Activity Is Dependent on the Folate Status.” New Phytologist 182 (1): 137–145.
Chicago author-date (all authors)
Van Wilder, Valérie, Veerle De Brouwer, Karen Loizeau, Bernadette Gambonnet, Catherine Albrieux, Dominique Van Der Straeten, Willy Lambert, Roland Douce, Maryse A. Block, Fabrice Rebeille, and Stephane Ravanel. 2009. “C1 Metabolism and Chlorophyll Synthesis: The Mg-protoporphyrin IX Methyltransferase Activity Is Dependent on the Folate Status.” New Phytologist 182 (1): 137–145.
Vancouver
1.
Van Wilder V, De Brouwer V, Loizeau K, Gambonnet B, Albrieux C, Van Der Straeten D, et al. C1 metabolism and chlorophyll synthesis: the Mg-protoporphyrin IX methyltransferase activity is dependent on the folate status. NEW PHYTOLOGIST. 2009;182(1):137–45.
IEEE
[1]
V. Van Wilder et al., “C1 metabolism and chlorophyll synthesis: the Mg-protoporphyrin IX methyltransferase activity is dependent on the folate status,” NEW PHYTOLOGIST, vol. 182, no. 1, pp. 137–145, 2009.
@article{533109,
  abstract     = {{Tetrahydrofolate derivatives are central cofactors of C1 metabolism. Using methotrexate as a specific inhibitor of folate biosynthesis, we altered the folate status in 10-d-old etiolated pea (Pisum sativum) leaves and followed the rate of chlorophyll synthesis upon illumination. 
In our conditions, the folate concentration decreased only from 5.7 to 4.2 nmol g(-1) FW, but the amount of chlorophyll after 24 h of illumination was reduced 2.5 times. Folate status and rate of chlorophyll synthesis were apparently correlated through the methyl cycle.
Indeed, we observed that methyl-tetrahydrofolate was the folate derivative most affected by the treatment; the decrease of methyl-tetrahydrofolate was associated with a sharp rise in homocysteine and S-adenosylhomocysteine concentrations, which are normally maintained at very low values, shifting the methylation index (S-adenosylmethionine/S-adenosylhomocysteine ratio) from 7 to 1; the decrease of the methylation index reduced by a factor of 3 the activity of the Mg-protoporphyrin IX methyltransferase (CHLM), an essential enzyme for chlorophyll synthesis. CHLM gene expression and protein concentration remained unchanged, suggesting that this inhibition relied essentially on metabolic regulation.}},
  author       = {{Van Wilder, Valérie and De Brouwer, Veerle and Loizeau, Karen and Gambonnet, Bernadette and Albrieux, Catherine and Van Der Straeten, Dominique and Lambert, Willy and Douce, Roland and Block, Maryse A. and Rebeille, Fabrice and Ravanel, Stephane}},
  issn         = {{0028-646X}},
  journal      = {{NEW PHYTOLOGIST}},
  keywords     = {{ARABIDOPSIS,EXPRESSION,PLANT DEVELOPMENT,GLYCINE DECARBOXYLASE,S-ADENOSYLMETHIONINE,ONE-CARBON METABOLISM,ADENOSYL-L-METHIONINE,ENHANCEMENT,CYSTATHIONINE GAMMA-SYNTHASE,GENE}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{137--145}},
  title        = {{C1 metabolism and chlorophyll synthesis: the Mg-protoporphyrin IX methyltransferase activity is dependent on the folate status}},
  url          = {{http://dx.doi.org/10.1111/j.1469-8137.2008.02707.x}},
  volume       = {{182}},
  year         = {{2009}},
}

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